I2D I2D lu-2012b-1 Microsomal prostaglandin E synthase-1 promotes hepatocarcinogenesis through activation of a novel EGR1/β-catenin signaling axis. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu D., Han C., Wu T. Cancer - Interactions investigated in the context of cancer Nuclear extracts of Huh-7 cells were obtained and subjected to immunoprecipitation followed by western blotting analysis. Author confirms that all constructs are of human origin. twu@tulane.edu Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database lu-2012b-2 Microsomal prostaglandin E synthase-1 promotes hepatocarcinogenesis through activation of a novel EGR1/β-catenin signaling axis. human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu D., Han C., Wu T. Cancer - Interactions investigated in the context of cancer Nuclear extracts of Huh-7 cells were obtained and subjected to immunoprecipitation followed by western blotting analysis. Author confirms that all constructs are of human origin. twu@tulane.edu Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database lu-2012b-3 Microsomal prostaglandin E synthase-1 promotes hepatocarcinogenesis through activation of a novel EGR1/β-catenin signaling axis. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu D., Han C., Wu T. Cancer - Interactions investigated in the context of cancer Nuclear extracts of Huh-7 cells were obtained and subjected to immunoprecipitation followed by western blotting analysis. Author confirms that all constructs are of human origin. twu@tulane.edu Oncogene (0950-9232) 2012 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL gsk3b_human Glycogen synthase kinase-3 beta GSK3B Serine/threonine-protein kinase GSK3B protein protein human Homo sapiens Human MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST lef1_human Lymphoid enhancer-binding factor 1 LEF1 T cell-specific transcription factor 1-alpha protein protein human Homo sapiens Human MPQLSGGGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESASGTGPRMTAAYI egr1_human Early growth response protein 1 EGR1 KROX24 ZNF225 AT225 Nerve growth factor-induced protein A Transcription factor ETR103 Transcription factor Zif268 Zinc finger protein 225 Zinc finger protein Krox-24 protein protein human Homo sapiens Human MAAAKAEMQLMSPLQISDPFGSFPHSPTMDNYPKLEEMMLLSNGAPQFLGAAGAPEGSGSNSSSSSSGGGGGGGGGSNSSSSSSTFNPQADTGEQPYEHLTAESFPDISLNNEKVLVETSYPSQTTRLPPITYTGRFSLEPAPNSGNTLWPEPLFSLVSGLVSMTNPPASSSSAPSPAASSASASQSPPLSCAVPSNDSSPIYSAAPTFPTPNTDIFPEPQSQAFPGSAGTALQYPPPAYPAAKGGFQVPMIPDYLFPQQQGDLGLGTPDQKPFQGLESRTQQPSLTPLSTIKAFATQSGSQDLKALNTSYQSQLIKPSRMRKYPNRPSKTPPHERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDICGRKFARSDERKRHTKIHLRQKDKKADKSVVASSATSSLSSYPSPVATSYPSPVTTSYPSPATTSYPSPVPTSFSSPGSSTYPSPVHSGFPSPSVATTYSSVPPAFPAQVSSFPSSAVTNSFSASTGLSDMTATFSPRTIEIC itf2_human Transcription factor 4 Immunoglobulin transcription factor 2 SL3-3 enhancer factor 2 ITF2 SEF2 TCF4 BHLHB19 Class B basic helix-loop-helix protein 19 protein protein human Homo sapiens Human MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM ctnnb1-egr1 1351108 n/a 1351111 unspecified role unspecified role bait bait n/a 1351112 unspecified role unspecified role prey prey n/a 1351113 unspecified role unspecified role prey prey n/a 1351114 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role prey prey association association false false false 5c Huh-7 cells transfected with pCMV-mPGES-1 displayed reduced immunoprecipitation of GSK3Beta but increased levels of EGR1, TCF4 and LEF1. Anti-PGE2 antibody treatment partially reverses mPGES-1-mediated reduction of GSK-3Beta. ctnnb1-egr1-5 1351108 n/a 1351114 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role bait bait physical association physical association false false false 7f mPGES-1 increases β-catenin interaction with EGR1 ; this interaction is partially inhibited by GSK-3β. egr1-ctnnb1-1 1351108 n/a 1351111 unspecified role unspecified role prey prey n/a 1351114 unspecified role unspecified role bait bait physical association physical association false false false 7a mPGES-1 overexpression increases association between Beta-catenin and EGR1. ctnnb1-egr1-3 1351108 n/a 1351114 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role bait bait association association false false false 7b-1 mPGES-1 overexpression increases association between Beta-catenin and TCF4 and EGR1. tcf4-ctnnb1-5 1351108 n/a 1351111 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role bait bait physical association physical association false false false 7c lef1-ctnnb1-1 1351108 n/a 1351113 unspecified role unspecified role bait bait n/a 1351111 unspecified role unspecified role prey prey physical association physical association false false false 7d mPGES-1 overexpression increases association between Beta-catenin and LEF1. ctnnb1-egr1-4 1351108 n/a 1351112 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role bait bait n/a 1351114 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role prey prey association association false false false 7e mPGES-1 overexpression increases association between Beta-catenin and TCF4 and EGR1. egr1-ctnnb1 1351109 n/a 1351111 unspecified role unspecified role prey prey n/a 1351114 unspecified role unspecified role bait bait physical association physical association false false false 6a Overexpression of mPGES-1 increased nuclear β-catenin association with EGR1, whereas mPGES-1 knockdown decreased such an interaction. tcf4-ctnnb1-3 1351109 n/a 1351111 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role bait bait physical association physical association false false false 6b Overexpression of EGR1 or mPGES-1 increases association of TCF4 with Beta-catenin. ctnnb1-lef1-8 1351109 n/a 1351113 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role bait bait n/a 1351115 unspecified role unspecified role prey prey association association false false false 6c Association of LEF1, TCF4 and Beta-catenin is increased with over expression of mPGES-1 but reduced with RNAi to EGR1. ctnnb1-egr1-1 1351109 n/a 1351114 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role bait bait physical association physical association false false false 6d mPGES1 increases Beta-catenin binding to EGR1, but not Sumo-EGR1. lef1-ctnnb1-2 1351110 n/a 1351115 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role prey prey n/a 1351114 unspecified role unspecified role prey prey n/a 1351113 unspecified role unspecified role bait bait association association false false false 7b mPGES-1 overexpression increases association between Beta-catenin and LEF1 and EGR1. tcf4-ctnnb1-6 1351110 n/a 1351113 unspecified role unspecified role prey prey n/a 1351115 unspecified role unspecified role bait bait n/a 1351114 unspecified role unspecified role prey prey n/a 1351111 unspecified role unspecified role prey prey association association false false false 7b mPGES-1 overexpression increases association between Beta-catenin and TCF4 and EGR1.