NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home chen-2009d-1 BCL11A represses HBG transcription in K562 cells. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells emsa supershift electrophoretic mobility supershift assay emsa supershift weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Chen Z., Luo H.Y., Steinberg M.H., Chui D.H. david.chui@bmc.org Blood Cells Mol. Dis. (1079-9796) 2009 imex curation hbg2_human_dna HBG2 promoter region dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) CCAGGGGCCGGCGGCTGGCT bc11a_human B-cell lymphoma/leukemia 11A B-cell CLL/lymphoma 11A COUP-TF-interacting protein 1 Ecotropic viral integration site 9 protein homolog BCL11A CTIP1 EVI9 KIAA1809 ZNF856 Zinc finger protein 856 protein protein human Homo sapiens Human MSRRKQGKPQHLSKREFSPEPLEAILTDDEPDHGPLGAPEGDHDLLTCGQCQMNFPLGDILIFIEHKRKQCNGSLCLEKAVDKPPSPSPIEMKKASNPVEVGIQVTPEDDDCLSTSSRGICPKQEHIADKLLHWRGLSSPRSAHGALIPTPGMSAEYAPQGICKDEPSSYTCTTCKQPFTSAWFLLQHAQNTHGLRIYLESEHGSPLTPRVGIPSGLGAECPSQPPLHGIHIADNNPFNLLRIPGSVSREASGLAEGRFPPTPPLFSPPPRHHLDPHRIERLGAEEMALATHHPSAFDRVLRLNPMAMEPPAMDFSRRLRELAGNTSSPPLSPGRPSPMQRLLQPFQPGSKPPFLATPPLPPLQSAPPPSQPPVKSKSCEFCGKTFKFQSNLVVHRRSHTGEKPYKCNLCDHACTQASKLKRHMKTHMHKSSPMTVKSDDGLSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDDEEEEEEEEEEEEELTESERVDYGFGLSLEAARHHENSSRGAVVGVGDESRALPDVMQGMVLSSMQHFSEAFHQVLGEKHKRGHLAEAEGHRDTCDEDSVAGESDRIDDGTVNGRGCSPGESASGGLSKKLLLGSPSSLSPFSKRIKLEKEFDLPPAAMPNTENVYSQWLAGYAASRQLKDPFLSFGDSRQSPFASSSEHSSENGSLRFSTPPGELDGGISGRSGTGSGGSTPHISGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACAQSSKLTRHMKTHGQVGKDVYKCEICKMPFSVYSTLEKHMKKWHSDRVLNNDIKTE bcl11a-hbg2 1456428 n/a HBG2 promoter region 1456429 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a bcl11a 1456430 unspecified role unspecified role bait bait human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells physical association physical association false false false Fig 6 Q9H165 decreases expression at RNA level of ENSG00000196565 (decreases activity of regulatory region as measured in reporter gene assay in K562-cells) - shown in Figure 4 and Figure 5 Q9H165 decreases expression at RNA level of ENSG00000196565