IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kadaba-2008-1 The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu Science (0036-8075) 2008 Only protein-protein interactions imex curation kadaba-2008-2 The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu Science (0036-8075) 2008 Only protein-protein interactions imex curation metn_ecoli Methionine import ATP-binding protein MetN metN abc b0199 JW0195 protein protein ecoli Escherichia coli (strain K12) MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGKSTLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGNVALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPKVLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGELIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV meti_ecoli D-methionine transport system permease protein MetI metI yaeE b0198 JW0194 protein protein ecoli Escherichia coli (strain K12) MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) meti-metn-1 1441091 n/a 1441093 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1441094 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1, Table S1. 3.7 A R = 0.31; Rwork = 0.31; Rfree = 0.35 metn-1 1441091 n/a 1441093 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 3b, Table S2 2.1 A R = 0.19; Rwork = 0.19; Rfree = 0.24 meti-metn-4 1441092 n/a 1441095 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1441093 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P30750:p.Glu166Gln mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false c2_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1441094 ancillary ancillary neutral component neutral component ecoli Escherichia coli (strain K12) atpase reaction atpase reaction false false false Fig.s 4, S3 L-methionine & L-selenomethionine (concentration-dependent) The MetN-C2 construct (aa 247-343 + 6xHis) showed a L-methionine-concentration-independent reduction of ATPase activity of ~50%.