UniProt UniProt http://www.uniprot.org harraz-2008-5 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation harraz-2008-1 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. mouse-heart Mus musculus heart [Cardiac; Cardiac tissue] heart Heart [Cardiac; Cardiac tissue] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation harraz-2008-2 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. mouse-kidney Mus musculus kidney kidney [Renal] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation harraz-2008-3 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation harraz-2008-4 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation harraz-2008-6 SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. mouse-fibroblast Mus musculus fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Harraz M.M., Marden J.J., Zhou W., Zhang Y., Williams A., Sharov V.S., Nelson K., Luo M., Paulson H., Schöneich C., Engelhardt J.F. john-engelhardt@uiowa.edu J. Clin. Invest. (0021-9738) 2008 Only protein-protein interactions imex curation rac1_bovin Ras-related C3 botulinum toxin substrate 1 RAC1 p21-Rac1 protein protein bovin Bos taurus (Bovine) Bovine MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL sodc_bovin Superoxide dismutase [Cu-Zn] SOD1 protein protein bovin Bos taurus (Bovine) Bovine MATKAVCVLKGDGPVQGTIHFEAKGDTVVVTGSITGLTEGDHGFHVHQFGDNTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLACGVIGIAK sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL sodc_mouse Superoxide dismutase [Cu-Zn] Sod1 protein protein mouse Mus musculus Mouse MAMKAVCVLKGDGPVQGTIHFEQKASGEPVVLSGQITGLTEGQHGFHVHQYGDNTQGCTSAGPHFNPHSKKHGGPADEERHVGDLGNVTAGKDGVANVSIEDRVISLSGEHSIIGRTMVVHEKQDDLGKGGNEESTKTGNAGSRLACGVIGIAQ rac1_mouse Ras-related C3 botulinum toxin substrate 1 Rac1 p21-Rac1 protein protein mouse Mus musculus Mouse MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL eea1_mouse Early endosome antigen 1 Eea1 protein protein mouse Mus musculus Mouse MFRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYQAVHDAGNDSGHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKSDGLVTDSSAELQALEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEEKSLRAAAEQKVTHLTEDLNKQTTVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSEHAHLEATINQLRSELAKGPQEVAVYVQEIQKLKGSINELTQKNQNLTEKLQKKDLDYTHLEEKHNEESASRKTLQASLHQRDLDCQQLQARLTASESSLQRAQGELSEKAEAAQKLREELREVESTRQHLKVEVKQLQQQREEKEQHGLQLQGEVSQLHCKLLETERQLGEAHGRLKEQRQLSSEKLMEKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLEKSKQQHQEQQALQQSATAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKGKESVSLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQQQLTQLTEKLKNQSESHKQAEENLHDQVQEQKAHLRAAQDRVLSLETSVSELSSQLNESKEKVSQLDIQIKAKTELLLSAEAAKAAQRADLQNHLDTAQHALQDKQQELNKVSVQLDQLTAKFQEKQEHCIQLESHLKDHKEKHLSLEQKVEDLEGHIKKLEADALEVKASKEQALQSLQQQRQLSTDLELRNAELSRELQEQEEVVSCTKLDLQNKSEILENIKQTLTKKEEENVVLKQEFEKLSQDSKTQHKELGDRMQAAVTELTAVKAQKDALLAELSTTKEKLSKVSDSLKNSKSEFEKENQKGKAAVLDLEKACKELKHQLQVQAESALKEQEDLKKSLEKEKETSQQLKIELNSVKGEVSQAQNTLKQKEKDEQQLQGTINQLKQSAEQKKKQIEALQGEVKNAVSQKTVLENKLQQQSSQAAQELAAEKGKLSALQSNYEKCQADLKQLQSDLYGKESELLATRQDLKSVEEKLTLAQEDLISNRNQIGNQNKSIQELQAAKASLEQDSAKKEALLKEQSKALEDAQREKSVKEKELVAEKSKLAEMEEIKCRQEKEITKLNEELKSHKQESIKEITNLKDAKQLLIQQKLELQGRVDSLKAALEQEKESQQLMREQVKKEEEKRKEEFSEKEAKLHSEIKEKEAGMKKHEENEAKLTMQVTTLNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKALELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMSCGKCFSVTVRRHHCRQCGNIFCAECSTKNALTPSSKKPVRVCDACFNDLQG cy24b_mouse Cytochrome b-245 heavy chain Cybb Cgd CGD91-phox Cytochrome b(558) subunit beta Heme-binding membrane glycoprotein gp91phox Neutrophil cytochrome b 91 kDa polypeptide gp91-1 gp91-phox p22 phagocyte B-cytochrome protein protein mouse Mus musculus Mouse MGNWAVNEGLSIFVILVWLGLNVFLFINYYKVYDDGPKYNYTRKLLGSALALARAPAACLNFNCMLILLPVCRNLLSFLRGSSACCSTRIRRQLDRNLTFHKMVAWMIALHTAIHTIAHLFNVEWCVNARVGISDRYSIALSDIGDNENEEYLNFAREKIKNPEGGLYVAVTRLAGITGIVITLCLILIITSSTKTIRRSYFEVFWYTHHLFVIFFIGLAIHGAERIVRGQTAESLEEHNLDICADKIEEWGKIKECPVPKFAGNPPMTWKWIVGPMFLYLCERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFFSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASILKSVWYKYCDNATSLKLKKIYFYWLCRDTHAFEWFADLLQLLETQMQERNNANFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASEHPNTTIGVFLCGPEALAETLSKQSISNSESGPRGVHFIFNKENF rac1-sod1-4 1385259 n/a 1385265 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false GTP-bound form n/a 1385266 unspecified role unspecified role prey prey purified purified direct interaction direct interaction false false false 2B, C, D and E, 3E rac1-sod1-5 1385259 n/a 1385265 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false GTP-bound form n/a 1385266 unspecified role unspecified role prey prey purified purified direct interaction direct interaction false false false 2F rac1-sod1-8 1385259 n/a 1385267 unspecified role unspecified role prey prey purified purified P00441:p.Leu9Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P00441:p.Gly11Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1385268 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Prereduced; GTP-bound form. direct interaction direct interaction false false false 3J rac1-sod1 1385260 n/a 1385269 unspecified role unspecified role prey prey n/a 1385270 unspecified role unspecified role bait bait physical association physical association false false false 2A rac1-sod1-1 1385261 n/a 1385269 unspecified role unspecified role prey prey n/a 1385270 unspecified role unspecified role bait bait physical association physical association false false false 2A rac1-sod1-2 1385262 n/a 1385270 unspecified role unspecified role bait bait n/a 1385269 unspecified role unspecified role prey prey physical association physical association false false false 2A rac1-sod1-3 1385263 n/a 1385270 unspecified role unspecified role bait bait n/a 1385269 unspecified role unspecified role prey prey physical association physical association false false false 2A cybb-eea1 1385264 n/a 1385271 unspecified role unspecified role neutral component neutral component n/a 1385270 unspecified role unspecified role neutral component neutral component n/a 1385269 unspecified role unspecified role neutral component neutral component n/a 1385272 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3F