UniProt UniProt http://www.uniprot.org terai-2006a-1 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation terai-2006a-2 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation terai-2006a-3 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation terai-2006a-4 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation terai-2006a-5 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation terai-2006a-6 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T. sasaki@basic.med.tokushima-u.ac.jp Mol. Biol. Cell (1059-1524) 2006 mimix curation milk2_mouse MICAL-like protein 2 Micall2 Jrab Junctional Rab13-binding protein Molecule interacting with CasL-like 2 protein protein mouse Mus musculus Mouse MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA rab13_human Ras-related protein Rab-13 RAB13 GIG4 Cell growth-inhibiting gene 4 protein protein protein human Homo sapiens Human MAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCSLG rab13-micall2 1435908 n/a 1435914 unspecified role unspecified role prey prey n/a 1435915 unspecified role unspecified role bait bait physical association physical association false false false Materials and methods rab13-micall2-1 1435909 n/a 1435914 unspecified role unspecified role prey prey n/a 1435915 unspecified role unspecified role bait bait physical association physical association false false false fig 1a rab13-micall2-2 1435910 n/a 1435915 unspecified role unspecified role bait bait n/a 1435914 unspecified role unspecified role prey prey physical association physical association false false false fig 1c micall2-rab13 1435911 n/a 1435914 unspecified role unspecified role neutral component neutral component n/a 1435915 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false fig 1D rab13-micall2-3 1435912 n/a 1435915 unspecified role unspecified role bait bait n/a 1435914 unspecified role unspecified role prey prey physical association physical association false false false Fig 7A rab13-micall2-4 1435913 n/a 1435915 unspecified role unspecified role bait bait n/a 1435914 unspecified role unspecified role prey prey colocalization colocalization false false false Fig 7B