HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html shinohara-2005-1 PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp J. Exp. Med. (0022-1007) 2005 imex curation Only protein-protein interactions imex curation shinohara-2005-2 PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp J. Exp. Med. (0022-1007) 2005 imex curation Only protein-protein interactions imex curation shinohara-2005-3 PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. mouse-b-cell Mus musculus B-cell b-cell B-cell [B lymphocyte; B-lymphocyte; B cell; B-cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse C57BL/6J spleen B cells Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp J. Exp. Med. (0022-1007) 2005 imex curation Only protein-protein interactions imex curation shinohara-2005-4 PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm ATCC Number:CRL-2111 chicken bursa lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara H., Yasuda T., Aiba Y., Sanjo H., Hamadate M., Watarai H., Sakurai H., Kurosaki T. Mouse primary B cell from spleen of C57BL/6J kurosaki@rcai.riken.jp J. Exp. Med. (0022-1007) 2005 imex curation Only protein-protein interactions imex curation m3k7_mouse Mitogen-activated protein kinase kinase kinase 7 Map3k7 Tak1 Transforming growth factor-beta-activated kinase 1 protein protein mouse Mus musculus Mouse MSTASAASSSSSSSASEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIVATAGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPARSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS q5f3w0_chick CARD11 RCJMB04_5k17 protein protein chick Gallus gallus (Chicken) Chicken MSTQGGEPEMDDCLETLKDEEEALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNSLMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEIIKLQQQVKTKDMQRCDLLAKSRQLEDERKQLKLNKIELMTFQERYNKMKEERNNYNDELVKVKDENYNLAMRYAQLSDEKSMAVMRSRDLQLEIDQLKHRLNKVEEECKLERNQSLKLKNDIENRPKKEQMLELERENEMMKTKIQELQSIIQADKRSLPDSDKAILDILEHDRKEALEDRHELVNKIFNLQEEIRHVEDLRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMIQLEEVEKERDQAFRSRDEAQTQYSHCLIEKDKYRKQIRELEERNDELRIEMVRKEACIVNLECKLRRLSKDNNCHDQSLPRNLPITIISQTFGNSSPKANGQEADDSSTSEDSPEDNKFFLPDQARLKRRVNLKGIQINPRAKSPVSMNRTSEFQAVRAQDEDGANASNGRTDTSSSNSVSISNSISSCEVSKIQTLRNRNDSIMSTTPEPPGNDSIVRRCKEDAPPCSMVEEDNDSFGFDALELDDDSHDRHSHGAPSVHSSSSSHQSEGLDAYDLEHVNSIFRKFSLERPFRPSVTSVGRIRSSCHTIQRITLNGDTLNSEITLIGGNDKGSFVSSVVSGSLAEKAGLREGHQLLLLEGCIKGENQSVPLDTCTKEEVHWTIQRCHGPVTLQYKSNHEGYRKLLSELEDGLIASGDSFHIRLNLNISSQLDCCSLSVKCDEIVHILDTMYQGTCGGCDWLCARVDPFTDKDLERGTIPSYSRAQQLLLVKLQRLMHRGSKDETESSHNTLRALRNTLQPEEPAPQSDPKTSPRLSRASFLLGQILQFVSRSENKYKRMNSNERVRIVTGWPSGLARTASEAKKPFPDKLEDLDSKSEINKRLIPYSLVRPIHCERRRPVLFTPTMLAKSLVQKLLNSGGALEFDICKPDIVTKEEFLRRQRMETIIFSREKNLSTYECIVPANIEAVSAKNKHCLLEAGISCTKDLIKAKIYPIVLFIRVSEKNIKRFRKLLPKPEAEDEFLRMCRLKEKELEELPCLYASVEADAWSSIEDLIRTIKDKIGEEQRKTIWIDEDQL car11_mouse Caspase recruitment domain-containing protein 11 Card11 CARD-containing MAGUK protein 1 Carma1 protein protein mouse Mus musculus Mouse MPGGGPAMDDYMETLKDEEEALWDNVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPRKEQVLELERENEMLKTKIQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNKIYNLQEEVRQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDNGSLDQSLPRHLPATIISQNLGDTSPRTNGQEADDSSTSEESPEDSKYFLPYHPPRRRMNLKGIQLQRAKSPISMKQASEFQALMRTVKGHEEDFTDGSPSSSRSLPVTSSFSKMQPHRSRSSIMSITAEPPGNDSIVRRCKEDAPHRSTVEEDNDSCGFDALDLDDENHERYSFGPPSIHSSSSSHQSEGLDAYDLEQVNLMLRKFSLERPFRPSVTSGGHVRGTGPLVQHTTLNGDGLITQLTLLGGNARGSFIHSVKPGSLAERAGLREGHQLLLLEGCIRGERQSVPLDACTKEEARWTIQRCSGLITLHYKVNHEGYRKLLKEMEDGLITSGDSFYIRLNLNISSQLDACSMSLKCDDVVHVLDTMYQDRHEWLCARVDPFTDQDLDTGTIPSYSRAQQLLLVKLQRLVHRGNREEADSAHHTLRSLRNTLQPEEMLSTSDPRVSPRLSRASFFFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLSRSSLDATKLLTEKHEELDPENELSRNLTLIPYSLVRAFHCERRRPVLFTPTMLAKTLVQKLLNSGGAMEFTICKSDIVTRDEFLRKQKTETIIYSREKNPNTFECIVPANIEAVAAKNKHCLLEAGIGCVRDLIKCKVYPIVLLIRVSEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEAEMWSSVEELLRVLKDKIVEEQRKTIWVDEDQL f1nkg4_chick BCL10 protein protein chick Gallus gallus (Chicken) Chicken SSAVGRPLTEDEMADVKKEALERMRPYLCDKIIAERHFDYLRSKKILTREDTEEISSRSSSRKKTGKLLDYLAENPKGLDALVESIRRERTQNFLLQKITDIVLKVKNEKLEALKGLSCSTCMTSLYGGTNNLSRSYSDESNFFDKTKDKESTQMHNPEEDYSTAAFMSAVSLHSMNLPVAEMGNAESAVFSVTLPGPGDPGAPPLPPELQSEQQEPCTSSSDNCFLPLRSRSLHPQ The sequence has been withdrawn from uniprot. e1c736_chick MALT1 protein protein chick Gallus gallus (Chicken) Chicken AVSRFLQLLEKGVYAIFYYAGHGYEHLGRNYMVPVDAPQLYAPENCISVQRILQKMQQQQTALNLILLDTCRKWYNPECALSQVQPLEPWGNTVYGYATSEDAEAYELQNGEFSSGIFMKYLKKHILQEKKVTHMLEDVLEDLGRDPVVTGKQVMEIKHTLKEARSLTDPICPTGAATERWGCSREPPSETVTFPCGVQAELRFHRLFSNVLSVCAKLQDLPAHLTDAQL The sequence has been withdrawn from uniprot. map3k7-2 1383785 n/a TAK1 1383789 putative self putative self putative self putative self mouse-b-cell Mus musculus B-cell b-cell B-cell [B lymphocyte; B-lymphocyte; B cell; B-cells] phosphorylation phosphorylation reaction phosphorylation false true false Figure 2A tak1-ikkb-2 1383785 n/a TAK1 1383789 enzyme enzyme unspecified role unspecified role nucl transfection nucleic acid transfection nucl transfection tak1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. n/a GST-IKKB 1383790 enzyme target enzyme target substrate substrate unspecified role unspecified role nucl transfection nucleic acid transfection nucl transfection ikkb_gsttag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O14920:p.[Ser177Ala;Thr180Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation false false false Figure 5D GST-IKKB which is S177 and S181 mutated to A177 and A181 shows no phosphorylation. tak1-carma1-3 1383786 n/a CARMA1 1383791 unspecified role unspecified role prey prey n/a TAK1 1383789 unspecified role unspecified role bait bait required to bind required to bind necessary binding region required to bind western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false tak1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Mouse TAK1 dB mutant-COOH terminal deleted (aa 531-579) physical association physical association false false false Figure 4A, 5B tak1-carma1-2 1383787 n/a CARMA1 1383792 unspecified role unspecified role bait bait n/a TAK1 1383789 unspecified role unspecified role prey prey physical association physical association false false false Figure 4B carma1-malt1-1 1383788 n/a BCL10 1383793 unspecified role unspecified role prey prey n/a CARMA1 1383791 unspecified role unspecified role bait bait n/a MALT1 1383794 unspecified role unspecified role prey prey association association false false false Figure 6C