IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ seitz-2000-1 The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli. in vitro In vitro solid phase assay solid phase assay western blot western blot Immuno blot Immuno blot Seitz H., Weigel C., Messer W. messer@molgen.mpg.de Mol. Microbiol. (0950-382X) 2000 Only protein-protein interactions imex curation dnaa_ecoli Chromosomal replication initiator protein DnaA dnaA b3702 JW3679 protein protein ecoli Escherichia coli (strain K12) MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS dnab_ecoli Replicative DNA helicase dnaB groP grpA b4052 JW4012 protein protein ecoli Escherichia coli (strain K12) MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE dnab-dnaa 1363771 n/a 1363772 unspecified role unspecified role prey prey dnab binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1363773 unspecified role unspecified role bait bait n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false dnaa binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1363779 1363776 physical association physical association false false false Tab. 1a N-terminal region of DnaB (1-156aa) does interact with N-terminal region of DnaA (1-86aa) but not with deletion mutant DnaA missing aa135-148 (EBI-6508320). dnab-dnaa-2 1363771 n/a 1363772 unspecified role unspecified role prey prey dnab binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1363773 unspecified role unspecified role bait bait c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false dnaa binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1363785 1363782 physical association physical association false false false Tab. 1a Full length DnaB does not interact with N-terminal DnaA 1-87 but does interact with DnaA missing aa135-148 (EBI-6508243). dnaa-dnab 1363771 n/a 1363772 unspecified role unspecified role bait bait dnab binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro n/a 1363773 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false Tab. 1b, Fig. 5