IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ thielens-1990-2 Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. in vitro In vitro equilib. dialysis equilibrium dialysis equilib. dialysis predetermined predetermined participant predetermined Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. J. Biol. Chem. (0021-9258) 1990 Only protein-protein interactions imex curation thielens-1990-1 Ca2+ binding properties and Ca2(+)-dependent interactions of the isolated NH2-terminal alpha fragments of human complement proteases C1-r and C1-s. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Thielens N.M., Aude C.A., Lacroix M.B., Gagnon J., Arlaud G.J. C1S andC1R were purified from human serum by column chromatography and identity plus length of the alpha fragments were established by Edman sequencing and amino acid content. J. Biol. Chem. (0021-9258) 1990 Only protein-protein interactions imex curation c1s_human Complement C1s subcomponent C1S C1 esterase Complement component 1 subcomponent s protein protein human Homo sapiens Human MWCIVLFSLLAWVYAEPTMYGEILSPNYPQAYPSEVEKSWDIEVPEGYGIHLYFTHLDIELSENCAYDSVQIISGDTEEGRLCGQRSSNNPHSPIVEEFQVPYNKLQVIFKSDFSNEERFTGFAAYYVATDINECTDFVDVPCSHFCNNFIGGYFCSCPPEYFLHDDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED c1r_human Complement C1r subcomponent C1R Complement component 1 subcomponent r protein protein human Homo sapiens Human MWLLYLLVPALFCRAGGSIPIPQKLFGEVTSPLFPKPYPNNFETTTVITVPTGYRVKLVFQQFDLEPSEGCFYDYVKISADKKSLGRFCGQLGSPLGNPPGKKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAVDLDECASRSKSGEEDPQPQCQHLCHNYVGGYFCSCRPGYELQEDTHSCQAECSSELYTEASGYISSLEYPRSYPPDLRCNYSIRVERGLTLHLKFLEPFDIDDHQQVHCPYDQLQIYANGKNIGEFCGKQRPPDLDTSSNAVDLLFFTDESGDSRGWKLRYTTEIIKCPQPKTLDEFTIIQNLQPQYQFRDYFIATCKQGYQLIEGNQVLHSFTAVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSRESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGALLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIALLELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRMDVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKEMEEED calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) c1r-c1s-3 1313683 n/a 1313685 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum n/a 1313686 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum n/a 1313687 unspecified role unspecified role neutral component neutral component 1313692 1313690 physical association physical association false false false 1313683 Fig. 7D, 7E 1.87 calcium atoms/mol. The full length proteins bound 4.05 calcium atoms/mol with a Kd = 1.94E-5M. c1s-calcium 1313683 n/a 1313687 unspecified role unspecified role neutral component neutral component n/a 1313685 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum direct interaction direct interaction false false false 1313683 Fig. 7A, 7B C1S alpha fragment bound 2.96 atom/mol of calcium c1r-c1s-4 1313683 n/a 1313686 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum n/a 1313687 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 7C Repeated equilibrium dialysis experiments performed with native C1R showed that this protein was able to bind Ca*+ in the 10-100uM CaCl2 concentration range, but Scatchard analysis of the binding was not possible, due to the high dispersion of experimental values. c1s-c1s 1313684 n/a 1313685 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum n/a 1313685 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum 1313706 1313704 direct interaction direct interaction false false false Fig. 6B Interaction is Calcium dependent. c1r-c1s-1 1313684 n/a 1313686 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum n/a 1313685 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum 1313711 1313709 direct interaction direct interaction false false false Fig. 6A, 6C, 6D Interaction is Calcium dependent.