UniProt UniProt http://www.uniprot.org yasui-2012-2 The Phytochrome-Interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yasui Y., Mukougawa K., Uemoto M., Yokofuji A., Suzuri R., Nishitani A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp cDNA library constructed from mRNA purified from the aerial portions of wild-type plants at bolting Plant Cell (1040-4651) 2012 Only protein-protein interactions imex curation yasui-2012-1 The Phytochrome-Interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yasui Y., Mukougawa K., Uemoto M., Yokofuji A., Suzuri R., Nishitani A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp cDNA library constructed from mRNA purified from the aerial portions of wild-type plants at bolting Plant Cell (1040-4651) 2012 Only protein-protein interactions imex curation yasui-2012-4 The Phytochrome-Interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Yasui Y., Mukougawa K., Uemoto M., Yokofuji A., Suzuri R., Nishitani A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp cDNA library constructed from mRNA purified from the aerial portions of wild-type plants at bolting Plant Cell (1040-4651) 2012 Only protein-protein interactions imex curation yasui-2012-3 The Phytochrome-Interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis. in vitro In vitro pull down pull down autoradiography autoradiography Yasui Y., Mukougawa K., Uemoto M., Yokofuji A., Suzuri R., Nishitani A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp cDNA library constructed from mRNA purified from the aerial portions of wild-type plants at bolting Plant Cell (1040-4651) 2012 Only protein-protein interactions imex curation yasui-2012-5 The Phytochrome-Interacting VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 Redundantly Regulate Flowering in Arabidopsis. arath-seedling Arabidopsis thaliana seedling anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yasui Y., Mukougawa K., Uemoto M., Yokofuji A., Suzuri R., Nishitani A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp cDNA library constructed from mRNA purified from the aerial portions of wild-type plants at bolting Plant Cell (1040-4651) 2012 Only protein-protein interactions imex curation phyb_arath Phytochrome B PHYB HY3 OOP1 Protein LONG HYPOCOTYL 3 Protein OUT OF PHASE 1 MSF3.17 At2g18790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRMACPGEGLPPELVRDMFHSSRWTSPEGLGLSVCRKILKLMNGEVQYIRESERSYFLIILELPVPRKRPLSTASGSGDMMLMMPY voz2_arath Transcription factor VOZ2 VOZ2 Protein VASCULAR PLANT ONE-ZINC FINGER 2 MHK10.12 At2g42400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNHPKITSAHQNVEEKLRELQERFCHLQAARKEGRHGDLALLEAQISQNIREWQAELTAPSPESSLLGEGISQFLEEFAPLLKLDEEDDATSTLKEHAGAKPDPEGFSQSLCPPEWTSENFSQSPFNGNFSCGFEDALNSTETHGQQLHYGYEGFDPSINSAPDFHDQKLSSNLDITSQYDYIFSEVRQELDNSPSTKLDSSEEIDNFAEFSTPSSVRVPPSAFLGPKCALWDCTRPAQGSEWYLDYCSNYHGTLALNEDSPGTAPVLRPGGISLKDNLLIDALRAKTQGKNVGIPVCEGAVNTKCPWNAAELFHLELVEGETIREWLFFDKPRRAYDSGNRKQRSLPDYSGRGWHESRKQLMKEQEGQKRSYYMDPQPPGPFEWHLFEYQINESDACALYRLELKVGNGKKSPKGKISKDPLADLQKKMGQFKVASDKPSPPTKGRKE voz1_arath Transcription factor VOZ1 VOZ1 Protein VASCULAR PLANT ONE-ZINC FINGER 1 F1K23.24 F3M18.4 At1g28520 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQQGGTLGSFSSDICRLLQLCDEEDDATSKLAAPKPEPADQNLEAGKAAVFQRGYNLVQGKSEHGLPLVDNCKDLSLAAGNNFDGTAPLEYHQQYDLQQEFEPNFNGGFNNCPSYGVVEGPIHISNFIPTICPPPSAFLGPKCALWDCPRPAQGFDWFQDYCSSFHAALAFNEGPPGMNPVVRPGGIGLKDGLLFAALSAKAGGKDVGIPECEGAATAKSPWNAPELFDLTVLESETLREWLFFDKPRRAFESGNRKQRSLPDYNGRGWHESRKQIMVEFGGLKRSYYMDPQPLHHFEWHLYEYEINKCDACALYRLELKLVDGKKTSKGKVSNDSVADLQKQMGRLTAEFPPENNTTNTTNNNKRCIKGRPKVSTKVATGNVQNTVEQANDYGVGEEFNYLVGNLSDYYIP pif3_arath Transcription factor PIF3 Phytochrome-associated protein 3 Basic helix-loop-helix protein 8 PIF3 PAP3 BHLH8 At1g09530 F14J9.19 Transcription factor EN 100 bHLH transcription factor bHLH008 Phytochrome-interacting factor 3 EN100 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLFELFRLTKAKLESAQDRNPSPPVDEVVELVWENGQISTQSQSSRSRNIPPPQANSSRAREIGNGSKTTMVDEIPMSVPSLMTGLSQDDDFVPWLNHHPSLDGYCSDFLRDVSSPVTVNEQESDMAVNQTAFPLFQRRKDGNESAPAASSSQYNGFQSHSLYGSDRARDLPSQQTNPDRFTQTQEPLITSNKPSLVNFSHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAKDSEDKVLNESVASATPKDNQKACLISEDSCRKDQESEKAVVCSSVGSGNSLDGPSESPSLSLKRKHSNIQDIDCHSEDVEEESGDGRKEAGPSRTGLGSKRSRSAEVHNLSERRRRDRINEKMRALQELIPNCNKVDKASMLDEAIEYLKSLQLQVQIMSMASGYYLPPAVMFPPGMGHYPAAAAAMAMGMGMPYAMGLPDLSRGGSSVNHGPQFQVSGMQQQPVAMGIPRVSGGGIFAGSSTIGNGSTRDLSGSKDQTTTNNNSNLKPIKRKQGSSDQFCGSS phyb-voz2-1 1319418 n/a 1319423 unspecified role unspecified role bait bait n/a 1319424 unspecified role unspecified role prey prey physical association physical association false false false Fig. S1A phyb-voz1-1 1319418 n/a 1319425 unspecified role unspecified role prey prey n/a 1319423 unspecified role unspecified role bait bait physical association physical association false false false Fig. S1A phyb-pif3-7 1319418 n/a 1319423 unspecified role unspecified role bait bait n/a 1319426 unspecified role unspecified role prey prey physical association physical association false false false Fig. S1A phyb-voz1 1319419 n/a 1319425 unspecified role unspecified role prey prey n/a 1319423 unspecified role unspecified role bait bait physical association physical association false false false phyb-voz2 1319419 n/a 1319424 unspecified role unspecified role prey prey n/a 1319423 unspecified role unspecified role bait bait physical association physical association false false false voz1-phyb-1 1319420 n/a 1319423 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1319425 unspecified role unspecified role neutral component neutral component region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig.6A voz2-phyb 1319420 n/a 1319423 unspecified role unspecified role neutral component neutral component region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1319424 unspecified role unspecified role neutral component neutral component region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig.6B voz1-phyb 1319421 n/a 1319423 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1319425 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. S1B voz2-phyb-1 1319422 n/a 1319424 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1319423 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6G