IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hulsmann-2012-1 The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de Cell (0092-8674) 2012 Only protein-protein interactions imex curation hulsmann-2012-2 The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hulsmann B.B., Labokha A.A., Gorlich D. goerlich@mpibpc.mpg.de Cell (0092-8674) 2012 Only protein-protein interactions imex curation imb_xenla Importin subunit beta p97 Importin-90 Karyopherin subunit beta kpnb1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELVTILEKTVSPDRNELEAAQKFLEQAAVENLPTFVVELSKVLANPANSQVARVAAGLQIKNPLTSRDPDVKAQYQQRWLAIDASARGEIKTYVLRTLGTESYRPSSASQCVAGIACAEITVNQWPQLIPQLVANVTDPNSTERMKESTLEAIGYICQDIDPEQLQHKSNEILTAIIQGMRKEEPSNNVRLAATNALLNSLEFTKANFDKESERHYIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITVEAMKNEIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPESCQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPSSYCLSSSFEVIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQVESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGAEFLKYMEAFKPFLTIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEMMQFLLENLGNENVHRSVKPQILSVFGDVALAIGGEFKKYLDVVLNTLQQASQAQVDKSDYDMVDYLNELREGCIEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDSVVACGAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKTKTLATWATKELRKLKNQA q91349_xenla nup62.L IL4I1 nup62 XELAEV_18038559mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFNFGAASAGGFSFGNPKSTTTTAPTGFSFGAATAAPSGGFSFGTATPTPASTTGQTSGLFSFSNPAPSLAPTSGFSFGAQVTSTPAPSSGGLAFGANTSKLNSGVGNQPAGGTTQTSQPMGGFSFGAATTQTQPSATSVGGFSFAGGVGSTSTNVFAQPAASTGITLQSAVSTAAAPTATTSQPTSTFSFGTQPQAAPALNFGLLSSSSVLSTASTPAAAQPVAPTTGLSLNFGKPADTSAAVTSTGSTTTNTPSLSSLLGTSGPSLFSSVATSTVPSVVSTVASGLSLTSTATSTGFGMKTLASSAVPTGTLATSTASLGVKAPLAGTIVQANAVGSAAATGISTATAMTYAQLENLINKWSLELEDQEKHFLQQATQVNAWDRTLMQNGERITTLHREMEKVKLDQKRLDQELDFILSQQKELEDLLTPLEESVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKEVIEHLNTSAGPGDASNPLQQICKILNAHMDSLQWIDQNSALLQRKVEQVTKECESRRKEQERGFSIAFD q9pvz2_xenla nup214.L CAN nup214 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MEDDTDLPPERETKDFQFRQLKKVRLFDYPADLPKQRSNLLVISNKYGLLFVGGFMGLKVFHTKDILVTVKPKENANKTVVGPQGIHVPMNSPIHHLALSSDNLTLSVCMTSAEQGSSVSFYDVRTLLNESKQNKMPFASCKLLRDPSSSVTDLQWNPTLPSMVAVCLSDGSISVLQVTDTVSVFANLPATLGVTSVCWSPKGKQLAVGKQNGTVVQYLPSLQEKKVIPCPSFYDSDNPVKVLDVLWLSTYVFTVVYAAADGSLEASPQLVIVTLPKKEDKRAERFLNFTETCYSICSERQHHFFLNYIEDWEILLAASAASVDVGVIARPPDQVGWEQWLLEDSSRAEMPMTENNDDTLPMGVALDYTCQLEVFISESQILPPVPVLLLLSTDGVLCPFHVVNLNQGVKPLTTSPEQLSLDGEREMKVVGGTAVSTPPAPLTSVSAPAPPASAAPRSAAPPPYPFGLSTASSGAPTPVLNPPASLAPAATPTKTTSQPAAAATSIFQPAGPAAGSLQPPSLPAFSFSSANNAANASAPSSFPFGAAMVSSNTAKVSAPPAMSFQPAMGTRPFSLATPVTVQAATAPGFTPTPSTVKVNLKDKFNASDTPPPATISSAAALSFTPTSKPNATVPVKSQPTVIPSQASVQPNRPFAVEAPQAPSSVSIASVQKTVRVNPPATKITPQPQRSVALENQAKVTKESDSILNGIREEIAHFQKELDDLKARTSRACFQVGSEEEKRQLRTESDGLHSFFLEIKETTESLRGEFSAMKIKNLEGFASIEDVQQRNKLKQDPKYLQLLYKKPLDPKSETQMQEIRRLNQYVKNAVQDVNDVLDLEWDQYLEEKQKKKGIIIPERETLFNSLANHQEIINQQRPKLEQLVENLQKLRLYNQISQWNVPDSSTKSFDVELENMQKTLSQTAIDTQTKPQAKLPAKISPVKQSQLRNFLSKRKTPPVRSLAPANLSRSAFLAPSFFEDLDDVSSTSSLSDMADNDNRNPPPKEIERQETPPPESTPVRVPKHAPVARTTSVQPGLGTASLPFQSGLHPATSTPVAPSQSIRVIPQGADSTMLATKTVKHGAPNITAAQKAAVAAMRRQTASQIPAASLTESTLQTVPQVVNVKELKNNGPGPTIPTVIGPTVPQSAAQVIHQVLATVGSVSARQAAPAAPLKNPPASASSIAPQTWQGSAPNKPAAQAIPKSDPSASQAPAPSVSQVNKPVSFSPAAGGFSFSNVTSAPVTSALGSSSAGCAATARDSNQASSYMFGGTGKSLGSEGSFSFASLKPASSSSSSSVVEPTMSKPSVVTAASTTATVTSTTAASSKPGEGLFQGFSGGETLGSFSGLRVGQADEASKVEVAKTPTAAQPVKLPSNPVLFSFAGAPQPAKVGEAPSTTSSTSASLFGNVQLASAGSTASAFTQSGSKPAFTFGIPQSTSTTAGASSAIPASFQSLLVSAAPATTTPSAPINSGLDVKQPIKPLSEPADSSSSQQQTLTTQSAAEQVPTVTPAATTATALPPPVPTIPSTAEAKIEGAAAPAIPASVISSQTVPFTSTVLASQTPLASTPAGGPTSQVPVLVTTAPPVTTESAQTVSLTGQPVAGSSAFAQSTVTAASTPVFGQALASGAAPSPFAQPTSSSVSTSANSSTGFGTSAFGATGGNGGFGQPSFGQAPLWKGPATSQSTLPFSQPTFGTQPAFGQPAASTATSSAGSLFGCTSSASSFSFGQASNTSGTSTSGVLFGQSSAPVFGQSAAFPQAAPAFGSASVSTTTTASFGFGQPAGFASGTSGSLFNPSQSGSTSVFGQQPASSSGGLFGAGSGGASTVGLFSGLGAKPSQEAANKNPFGSPGSSGFGSAGASNSSNLFGNSGAKAFGFGGTSFGDKPSATFSAGGSVASQGFSFNSPTKTGGFGAAPVFGSPPTFGGSPGFGGSPAFGTAAAFSNTLGSTGGKVFGEGTSAATTGGFGFGSNSSTAAFGSLATQNTPTFGSISQQSPGFGGQSSGFSGFGAGPGAAAGNTGGFGFGVSNPTSPGFGCWRS nup93_xenla Nuclear pore complex protein Nup93 nup93 93 kDa nucleoporin An4a Nucleoporin Nup93 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDGEGFGELLQQAEQLAAETEGVTELPHVERNLQEIQQAGERLRSKTMTRTSQESANVKASVLLGSRGLDISHISQRLESLSAATTFEPLEPVKDTDIQGFLKNEKDNALLSAIEESRKRTFVMAEEYHRESMLVEWEQVKQRVLHTLLASGEDALDFTQESETSYISESGAPGRSSLDNVEMAYARQMYMYNEKVVSGHLQPSLVDLCTEAAERLDDKNVSDLWVMVKQMTDVPLIPASDTLKSRCSGQMQMAFVRQALNYLEQSYKNYTLISVFANLQQAQLGGVPGTYNLVRSFLNIRLPTPIPGLQDGEIEGYPVWALIYYCMRCGDLMAAQQVVNRAQHQLGDFKNCFQEYIHNKDRRLSPTTENKLRLHYRRAVRASTDPYKRAVYCIIGRCDVSDNHSEVADKTEDYLWLKLSQVCFEDEANSSPQDRLTLPQFQKQLFEDYGESHFAVNQQPYLYFQVLFLTAQFEAAIAFLFRLERTRCHAVHVALALFELKLLLKSTGQSAQLLSQEPGEPQGVRRLNFIRLLMLYTRKFEPTDPREALQYFYFLRNEKDNQGESMFLRCVSELVIESREFDMLLGKLEKDGSRKPGAIDKFTRDTKTIINKVASVAENKGLFEEAAKLYDLAKNPDKVLELTNKLLSPVVSQISAPQSNRERLKNMALAIAERYKSQGVSAEKSINSTFYLLLDLITFFDEYHAGHIDLSFDVIERLKLVPLSQDSVEERVAAFRNFSDEIRHNLSEILLATMNILFTQYKRLKGSGPTTLGRPQRVQEDKDSVLRSQARALITFAGMIPYRMSGDTNARLVQMEVLMN hsp70_xenla Heat shock 70 kDa protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MATKGVAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQCDLKHWPFQVVSDEGKPKVKVEYKGEEKSFFPEEISSMVLTKMKETAEAYLGHPVTNAVITVPAYFNDSQRQATKDAGVLAGLNILRIINEPTAAAIAYGLDKGARGEQNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRMVNHFVEEFKRKHKKDIGQNKRALRRLRTACDRAKRTLSSSSQASIEIDSLFEGIDFYTAITRARFEELCSDLFRGTLEPVEKALRDAKLDKSQIHEIVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKQTQSFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSAVEKSSGKQNKITITNDKGRLSKEDIEKMVQEAEKYKADDDAQRERVDAKNALESYAFNLKSMVEDENVKGKISDEDKRTISEKCTQVISWLENNQLAEKEEYAFQQKDLEKVCQPIITKLYQGGVPGGVPGGMPGSSCGAQARQGGNSGPTIEEVD q642r6_xenla nup205.S MGC83295 nup205 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAQLALNSEASLWGPYREIWQTVLSALIKRQPEAVHSLDIVLKKYKPDFISLFKNPPKSAQQHERVQKASTEGIPIKGTQRTRILEEQLIKEAFILSDLYNIGEIAAVELLLIGEQQQPTFHGLTRGLVAILLYWDGKSCMAESLLHLIQARKGKTFTLDHSPEVVSMVTRFTDDLMEQGLTNKILTLISQIDVNNEFDKLKKERGLGNKKHRKEVSDLIKECQQSLAHSLYSWSCQTPLNREDTLLLIGYLEKVTVEGDGSLDKVNLTLLMSLLYCLDVGFLEQGTDDREELMKQASMFMDRQYIAAIHNRLQNTQPWKSPGMQATVRLAWALALRGISQFSEVLEFSEADEPMAEIAIGGNVFLFLTEAVVGSESFCTDEFFIRRIHKLVTDFPTLMPMKVKQLRNRAEEDARLIQMSMQMGNEPPASLRRDLEHLLLLIGELYRKDPFHLELALEYWCPTEPLQSTSLMGSFLGVAHQRPPQRQVLLSKFVRQMSDLLPATLYLPYLKMLRGLASGPQCAHYCFSLLKANGGSSAENLQAAGGSPVSWDHFFHSLMLYHEHLRRDLPNTDNIHQRHPPLRGITQRELDGLIACLQLTCTIIDWSESARLALCEHAQWMPVVVILGLLQCSIPPLLKAELLKTLAAFGKSPEIAASLWQSLEYTQILQTVRATGLRQGVGIEVELNEIESRCEEYPLTRAFCQLISTLVESSFPTNLGAGLRAPGFEPYLQFLRDTVFLRYRTRAYRRAAEKWEVAEAVLDVFYKLLKDYEPQPEDFVDQYVELQGEERVAFKPPGFSLMHHLLNESPMLELCLSLMEEGVTQLDTYAPFPGKKHLEKAVAYCFMLLNLTLQKENRFMDLLRESHLSMIVTPLEQLLQGINPRSKKADNVVNIARYLCHGNSNAELAFESAKILCSISCNSKIQEKIVGDFTQDQNVSQKLMVGFVSCLDSEEAEELLDSEKEAEDQVKQTNIRYMTKIHILNLLITSLEMKAPNLAMFLLGYELKKPVSTTNLQDSGVLGCPRTCLHSILDILRKGTDVRAGPVAVWDTPHLAELCYQVIYQLCACADTSGPTMRYLRTSQDFLFSQLQHLPFSVEESEISAMNQMSWLMKTATIELRITSLNRQRSHTQRLLHLLLDDMPTRPYSADGEGGMEDESRSLSGFLHFDTTSKVRRKILRILDSIQFSNEIPEPLQLDFFDRSQIEQVIANCEHKNRRGQTVCNVKLLHRVLVAEVNALQGMAAIGQRPLLMEEINTILQYVVERNKLLQCLHAKRHALESWRQLVEIILTACPQDLIPTEHRQLIIRDLLQDLHVKILDDDAAQELMPIVAGAVFTLTAHLSQSVRTELKQPMTASGLGQSQYVQMLDGSFAAPPGTENISAGFASIGDSSLHMILRNLLEFILKTGGGFQRVRAHLYGSLLYYLQIAQRPDEPDTLESAHKSMWERLTAPEDVFSKLQRDNLSIFESYGTALMEVVCRDACDGHDIGRMLALALLDRIVSVDRQQQWLLYLSNSGYLKVLVDSLAEDDVVLRNLLTPQPPLLKALYIYESKMAFLTRVAKSSQGAIELLRSGVIVRLAQCQVYDMRPETDPHGVFGMRETPVFIPAPVERYRQILLPALQICQLILTSSTAQHLQAAGQVLQFLVAHSDTIQAILRSQEGSLGSLQELALLTGIISKAALPGVLNELDIGLNDGSMMELQGHIGRFQRQCLALLNRFGGSDRLRQLSLQDDSSRLDGVSKKDDMELAMQQICSNVMEYCQALMIQNSPSFQQTVCLFTPSLKESASRDGTRQDSQVSILPSWRLPSLGVVIHLLKQSANNFFTYYDIHRQSVGKLQNVEQLPPDEIKELCQSEMPVGADKISTTQKYGLARRRLVKLINSRAKLLSLCSYIIETCLYILWRHLEYYLLHCTTSDSQDPVFSNMTFGNRRFQDTFNTDPNMDPRNLRQNKVSQQDVDTLLREGANSFGESLQKRLLDIESLYCKVRSRHSFIQALVRRIRGLLRVSRV a4fu91_xenla nup58.L nup58 nupl1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MASGFAFAAAAGTATLNPAAGAVAPFSFGGTPAASNTGTTGGLAFGAFGAAATPATTTAATGLGGGLFGAKPATGFTLGGANTATATTTSASTGFSIGFNKPAGSATPFSLPVTSTSSGGLSLASALTSTPATGPSPFTLNLGSTPATTTAASIGLSLGGTLTGLGGSLFQNINPSTGLGQITLGQNTLGLGLNLGAVAPVSQATTHEGLGGLDFSNSSDKKSQKAGARPEDSKALKDENLPQLLCQDVENFQKFVKQQKQVQEEISRMSSKAMLKVQEDIKALKQLLSVASSGLQRNALAIDKLKIETAQELKNAEIALRTQKTPPGLQHENTAPAYYFHTLVQQFEVQLQQYRQQIEELENHLATQSNSLHLSPQDLSMAMQKLYQTFVALAAQLQAVNENFKMLKEQYLGYRKTFLGDSTDVFEARRAEAKKWQSAPRVTTGPTPFSNIPNAAAVAMAATLTQQQQPTTGPRPSLGVSFGTPFGSAMNAGLQPSGLGSSGLGGFGSSSAFGGNTSGSSSFGFGTANKPSGSLSAGFGSTSTSGFNFSNPGINASAGLTFGVSNPSSTGFGTAGQLLQLKKPPTGNKRGKR q6pay1_xenla nup54.S MGC68541 nup54 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAFNFGATTGTPANQGGFGGFGATTTASTGPAFSFTTPANTTSGLFGATQNKGFGFGTGFGSTTTSTGLGTGLGTGLGFTGFNTSQQQQQQSVLGAGLFNQSFQSTPQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGFFMNNTPPVEFTQENPFCRFKAVGFSYIPNNKDEDGMISLIFNKKESDIRGQQQQLVESLHKVLGGHQTLTVNVEGVKTKADNQTEVIIYVVERSPNGTSRRVGASALFSYFEQAHIKANMQQLGVTGAMAQTELSPVQIKQLIQNPLSGVDPIIWEQAKVDNPDPERLIPVPMIGFKELLRRLEVQDQMTKQHQSRLDIISEDIGELQKNQTTTMAKIGQYKRKLMELSHRVLQVLIKQEIQRKSGFAIQAEEEQLRVQLDTIQSELNAPTQFKGRLNELMSQIRMQNHFGAVRSEEKYYVDADLLREIKQHLKQQQEGVSHLISIIKDNHEDIKLIEQGLNDNLHMRTGFLS q6dcp4_xenla Nup98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MFNKTFGSPFGTGNGAFGATSTFGQTTGFGTTPAAAFGSAGFGTNTTTGGLFGNTQTKPGSLFGANTFNQPATSSASSGFGFGASTGTTNSLFGSTNTGSSLFSTPSNAFAQTKPASFGNFGTSTSTGGLFGNTNTANPFSGTSASLFGASTFSSAPTGTTIKFNPQSGTDTMAKGGVTTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNPVGAPAGTGLFGTSAATSSAPTGIFGSTAANSFSFAGNKTTFGAAGTGAFAANTGGLFGQPTNPPAASLFNKPFGNAATTQSTGFSFGNTSTLGQAQTSTMGLFGANQATQAGGLFGATTNTNAAGAFGAGSSLFGQPNPAPFGGGSTLFGNKPAGFGTTTTTAPAFGTTTGGLFGNKATLTLGTNTNTSNFGFGANAAGTSLFGNKPATGTIGPSLGTSFGTALNPGQTSLFGGNQPKLTGTLGTGAFGGTGFNSTSTGLGFGAPQPAMATLTDPNASAAQQMFLQQQYNALRYSPFPDSPLFRNPISDPKKKEELLKPTNPAAQKAVLTPTHYKLTPRPAARVRPKALQNSGAAKSQLFDALDDDEPVLSSGVFMPKKSIKKLDLKYLKNSSSLFAQESKDVNEVVPPTPETQDRSVENHHADEKKKK nup1_yeast Nucleoporin NUP1 NUP1 YOR098C YOR3182C Nuclear pore protein NUP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSNLSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLPILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSEYDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSNRLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTVGFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANKTKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKSAFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIASGNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNKPPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGAGGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR 4AC23567D2FB53CC nup62-kpnb1 1315227 n/a 1315229 unspecified role unspecified role prey prey n/a 1315230 unspecified role unspecified role bait bait n/a 1315231 unspecified role unspecified role prey prey n/a 1315232 unspecified role unspecified role prey prey n/a 1315233 unspecified role unspecified role prey prey n/a 1315234 unspecified role unspecified role prey prey n/a 1315235 unspecified role unspecified role prey prey n/a 1315236 unspecified role unspecified role prey prey association association false false false Supp. Fig. 1B Importin-alpha and Nup88 also identified but could not be ascribed to a single gene product. Bait antibody mouse monoclonal nup62-hsp70 1315227 n/a 1315229 unspecified role unspecified role prey prey n/a 1315233 unspecified role unspecified role prey prey n/a 1315231 unspecified role unspecified role prey prey n/a 1315232 unspecified role unspecified role prey prey n/a 1315234 unspecified role unspecified role prey prey n/a 1315235 unspecified role unspecified role prey prey n/a 1315230 unspecified role unspecified role bait bait n/a 1315236 unspecified role unspecified role prey prey association association false false false Supp. Fig. 1C Importin-alpha and Nup88 also identified but could not be ascribed to a single gene product. Bait antibody rabbit polyclonal nup54-nup93 1315227 n/a 1315229 unspecified role unspecified role prey prey n/a 1315235 unspecified role unspecified role prey prey n/a 1315230 unspecified role unspecified role prey prey n/a 1315234 unspecified role unspecified role prey prey n/a 1315232 unspecified role unspecified role prey prey n/a 1315236 unspecified role unspecified role bait bait association association false false false Supp. Fig. 1C Importin-alpha was also identified but could not be ascribed to a single gene product. Bait antibody mouse monoclonal nup98-kpnb1 1315228 n/a 1315237 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1315229 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig. 6B Mutation of all hydrophobic residues (Phe, Leu, and Ile) of the Nup98 FG domain to serines inhibited this interaction. nup1-kpnb1 1315228 n/a 1315229 unspecified role unspecified role prey prey n/a 1315238 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 6B Low levels of yeast NUP1 (2-175) also bound importin-beta.