UniProt UniProt http://www.uniprot.org oh-2012-1 Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Oh S.A., Allen T., Kim G.J., Sidorova A., Borg M., Park S.K., Twell D. psk@knu.ac.kr Plant J. (0960-7412) 2012 Only protein-protein interactions imex curation oh-2012-2 Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Oh S.A., Allen T., Kim G.J., Sidorova A., Borg M., Park S.K., Twell D. psk@knu.ac.kr Plant J. (0960-7412) 2012 Only protein-protein interactions imex curation oh-2012-3 Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Oh S.A., Allen T., Kim G.J., Sidorova A., Borg M., Park S.K., Twell D. psk@knu.ac.kr Arabidopsis spore preparations enriched for haploid microspores and early bicellular pollen cDNA. Plant J. (0960-7412) 2012 Only protein-protein interactions imex curation tio_arath Serine/threonine-protein kinase TIO TIO FU Fused homolog Protein TWO-IN-ONE F14I3.15 At1g50240 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGVEDYHVIELVGEGSFGRVYKGRRKYTGQTVAMKFIMKQGKTDKDIHSLRQEIEILRKLKHENIIEMLDSFENAREFCVVTEFAQGELFEILEDDKCLPEEQVQAIAKQLVKALDYLHSNRIIHRDMKPQNILIGAGSVVKLCDFGFARAMSTNTVVLRSIKGTPLYMAPELVKEQPYDRTVDLWSLGVILYELYVGQPPFYTNSVYALIRHIVKDPVKYPDEMSTYFESFLKGLLNKEPHSRLTWPALREHPFVKETQEEVEAREIHTAVVDNKAAWMLKGNGGQQRNEKCDSVTLVEDMSATKGLADVQSDMKSAVKVNSPPTEDFVGFPTQEEIKSSGNPTLDKLENTSRTVKGAQVIGENDKALDLVLLSLERFSKSPDSKRDKDVACSVQSLRIISNLVATRAIVSVGLIEKITCALLDFTDALVGMKSPEFNNIIPKSLSVTKNLVGHVEGNNIHSSYIRHWTKVVEIFIQVVRWEEEGTGRIIYEACSCITTMLSRVAQDLKSSTPDSVSKQILEHANMSRIVDHLCLCLASSGSSLTSGSSQMLAAACEACRAIWILIDTSETFFKNDDVNILPLDALQNRLSQHDIGNSEWGPLSEKLVDTVTRAYLRSKHVQVAVGHCLHQRVEAPLVSAIQLLSRCCLHNGILPSMLCGLPSSLPITTVVSGGEDGTVISEIFSILSYATLSSKDQQTGEKDNFEGRLNNLVFHSCLMLATVAQCLKLTGRNSVLLMLTTSPKKHQHRLSAIANHIASDDKIEASLQNHSASAMLALASILALEKGSSAGSSVSELVVSLIPRATKLCYHLRPMPSNEGEVISHSANYAKWHGLLDGCIGLLESRLKWGGPLAVQQLIASGTPLLLINLLAGKLSNASPEDIKKTSNRIGLSPIGVVWTISSICHCLSGGTTFRQVLVKIETMKLITCLLSDAHIKLVKSWGGPGGGKDGVRETINVIIDLLAFPFVALQSQPGSLSATASVNSGFILNIGSPGVRVCMEDRDLLKAIEEDMDKYIIVLLEVGVPSLILRCLDHLELKDLVRPVAFLAKMVGRPRLAVDLVSKGLLDPNRMKKLLNQSSPREVILDILMIISDLSRMDKAFYKYIGEASVLQPLKEYLTHVDPNIRAKACSALGNMCRHNGYFYSALAEHQIIGLLIDRCADPDKRTQKFACFAIGNAAYHNDTLYEELRRSITQLANVLTTAEEDKTKANAAGALSNLVRNSNKLCEDIVSKGALQTLLRLVADCSTLALNPSKKETASESPLKIALFSLAKMCSNHQICRQFVKSSELFPVIARLKQSPEANIAHYASVIVAKVSGES kn12a_arath Kinesin-like protein KIN-12A KIN12A PAKRP1 Phragmoplast-associated kinesin-related protein 1 FCAALL.159 dl3115c At4g14150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKKHFTLPRNAILRDGGEPHSPNPSISKSKPPRKLRSAKENAPPLDRNTSTPDHRSMRMKNPLPPRPPPSNPLKRKLSAETATESGFSDSGVKVIVRMKPLNKGEEGDMIVEKMSKDSLTVSGQTFTFDSIANPESTQEQMFQLVGAPLVENCLSGFNSSVFAYGQTGSGKTYTMWGPANGLLEEHLCGDQRGLTPRVFERLFARIKEEQVKHAERQLNYQCRCSLLEIYNEQITDLLDPSQKNLMIREDVKSGVYVENLTEEYVKNLTDVSQLLIKGLGNRRTGATSVNTESSRSHCVFTCVVESRCKNVADGLSSFKTSRINLVDLAGSERQKSTGAAGERLKEAGNINRSLSQLGNLINILAEISQTGKPRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAVVNEVMQDDVNFLRGVIHQLRDELQRMKNDGNNPTNPNVAYSTAWNARRSLNLLRSFGLGHPRSLPHEDNDGDIEMEIDEAAVERLCVQVGLQSSLASEGINHDMNRVKSIHSSDGQSIEKRLPEDSDVAMEDACCHTENHEPETVDNMRTETETGIRENQIKTHSQTLDHESSFQPLSVKDALCSSLNKSEDVSSCPDLVPQDVTSANVLIADGVDDPEHLVNSASPSLCIDPVGATPVLKSPTLSVSPTIRNSRKSLKTSELSTASQKDSEGENLVTEAADPSPATSKKMNNCSSALSTQKSKVFPVRTERLASSLHKGIKLLESYCQSTAQRRSTYRFSFKAPDSEPSTSISKADAGVQTIPGADAISEENTKEFLCCKCKCREQFDAQQMGDMPNLQLVPVDNSEVAEKSKNQVPKAVEKVLAGSIRREMALEEFCTKQASEITQLNRLVQQYKHERECNAIIGQTREDKIIRLESLMDGVLSKEDFLDEEFASLLHEHKLLKDMYQNHPEVLKTKIELERTQEEVENFKNFYGDMGEREVLLEEIQDLKLQLQCYIDPSLKSALKTCTLLKLSYQAPPVNAIPESQDESLEKTLEQERLCWTEAETKWISLSEELRTELEASKALINKQKHELEIEKRCGEELKEAMQMAMEGHARMLEQYADLEEKHMQLLARHRRIQDGIDDVKKAAARAGVRGAESRFINALAAEISALKVEKEKERQYLRDENKSLQTQLRDTAEAIQAAGELLVRLKEAEEGLTVAQKRAMDAEYEAAEAYRQIDKLKKKHENEINTLNQLVPQSHIHNECSTKCDQAVEPSVNASSEQQWRDEFEPLYKKETEFSNLAEPSWFSGYDRCNI kn12b_arath Kinesin-like protein KIN-12B KIN12B PAKRP1L Phragmoplast-associated kinesin-related protein 1-like protein MDB19.16 At3g23670 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKHFMMPRNAILRDIGESQSPNPSLTKSKSQRKIKSSKENAPPPDLNSLIPDHRSSPAKLKSPLPPRPPSSNPLKRKLIAEATADNGVAIGVSDSGVKVIVRMKPPSKGEEEEMIVKKISNDALTINEQTFTFDSIADPESTQDEIFQLVGAPLVENCLAGFNSSVFAYGQTGSGKTYTMWGPANGLLEEHLSGDQRGLTPRVFELLFARLSEEQAKHAERQLKYQCRCSFLEIYNEQITDLLDPSLKNLMIREDVKSGVYVENLTEEYVKNLKDLSKLLVKGLANRRTGATSVNAESSRSHCVFTCVVESHCKSVADGLSSFKTSRINLVDLAGSERQKLTGAAGDRLKEAGNINRSLSQLGNLINILAEISQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAIVNEVMQDDVNFLREVIRQLRDELQRVKDDKGNNPTNPNAAYTTSWNARRSLSLLRSFGLGHPKSLPNGDDDGDTEMEIDEEAVERLCAQMGLSPPAEDNNQEMSRVEKINSSLQTVVLKDESYNNSHLKSSEATDVNMEDACCQTENNGSETDNALTVAETMDDGSSVQPDSITNSLHSCISDTNQGNSPSKAENIPSCQDLVIEADVSAIVSVADTSNNTEQVSVNPVSPCLSVAPVSVSPVLIPPTESASPKIRNSRKSLRTTSMSTASQKDIERANQLTPEVVEPSPAMSTEVLNLYSALSTKKSEAFPVPTRQLAASLHRGMKLLDSYRQSTALRRSTFRLSYKALECKPSTVLSKADVGVQTYPQADEIAEDNSKEVLCSRCKCRAECDAQEISDTSNLQLVPIDNSEGSEKSNFQVPKAVEKVLAGSIRREMAMEEFCTKQASEISQLNRLVQQYKHERECNAIIGQTREDKIVRLESLMDGVLSKDDFLDEEFASLMHEHKLLKDMYENHPEVLQTRIELKRVQEELESFKNFYGDMGEREVLLEEIHDLKAQLQCYTDSSLTSARRRGSLLKLTYACDPNQAPQLNTIPESVDEGPEKTLEQERLRWTEAESNWISLAEELRTELDTNRLLMEKQKRELDTEKRCAEELTEAMQMAMQGHARMIEQYADLEEKHIQLLARHRRIREGIDDVKKAAARAGVKGAESRFINALAAEISALKVQREKEVRYFRDENKSLQSQLRDTAEAVQAAGELLVRFKEAEEGLTFAQKRAMDAEYEASEAYKKVDKLKRKYETEISTVNQQHNAEPQNPIESLQASCNDDAMAKYDEPSASDGDNQWREEFQPFYKKDEELSKLAEPSWFSGYDRCNI fu-pakrp1 1315116 n/a 1315119 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1315120 unspecified role unspecified role prey prey c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1315126 1315124 physical association physical association false false false Fig.2b, Fig. 3b, Fig.4b fu-kinesin_12b 1315116 n/a 1315121 unspecified role unspecified role prey prey c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1315119 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1315131 1315129 physical association physical association false false false Fig.2c, Fig. 3b, Fig.4b fu-pakrp1-1 1315117 n/a 1315120 unspecified role unspecified role neutral component neutral component region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1315119 unspecified role unspecified role neutral component neutral component c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig.2f fu-kinesin_12b-1 1315117 n/a 1315119 unspecified role unspecified role neutral component neutral component c-term cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1315121 unspecified role unspecified role neutral component neutral component region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig.2g fu-pakrp1-3 1315118 n/a 1315119 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1315120 unspecified role unspecified role prey prey c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1315148 1315146 physical association physical association false false false