mpidb The Microbial Protein Interaction Database boyd-2012-1 LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. psefl Pseudomonas fluorescens pull down pull down anti tag western anti tag western blot anti tag western Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation boyd-2012-2 LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG q3kk29_psepf lapA Pfl01_0133 protein protein psepf Pseudomonas fluorescens MTPGESSMSSVVAIVKSIVGQVFVVSPEGVRRVLVEGDRLFAGDQVDTGVSGAVSLELADGRTIDLGRDTQWSADTPDSSTDLAEATAQAAPSVEELQQAIAAGVDPTTALESTAAGPSAAGTGGAAGGGHSFVMLDATAGRVDPTIGFPTAGINAAGQAAQNITGGQNTDTTANALRESTLSLSATPSITEAGGVLVYTATLTQAPLTDLTITLSNGAVIVIPAGSTTGTANVPLAPNDTVYNDPTQIDVTVTGTTGGSGITVTPPTTPATTQVTDTIDTTTVTLTAGPSVTEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGTVNVPTAPNDVYNNGSTVTTTITGATGGNFENLVPNPTPAVTTITDSIDTTGLTLTATGTVVEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGSVNVPTAPNDVYNNGGTVSTTITGATGGNFENLVPNTTPATTTVTDSIDTTNLSLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKIEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTSTVKLTADSSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTVTTTAPNDALAGNAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADITNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDVLTGHAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADTTNLTLSATGTVAEGGSIVYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTTTVKLTATESAVEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTSVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTRVTDTVDTTNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDALNGHTPLSNSITGVTGGNYENLVADKTPVSTTVTDTVDTTDLTLTATDSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAVEGGTVSYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITNVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTAPAVTEVTDTIDTSTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITDVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATPEVNEGGQITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVETTISTTTGGNFENLVTSTAPAVTTVNDTIDTSTVSLTATANVAEGETVVYTATVTAPVTGSPVVVTLSNGQTITIAVGETTGTVNFVAPNSPLAGGSSLSVTIDKATGGNYENLAVDGKSADTSVSDTVDTTNLNLTATDSVAEGGSITYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGGFENLVPSTVPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTIGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTASETAAEGGTVTYTATVGAPVTGSPVTVTLANGQQITIEVGKTTGTVTTTAPNDVLNDHAPLTNAITDVSGGNYENLVADKTPVSTTITDTIDTTNLSLTATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGTVEATIKSATGGNFENLVTSTTPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLSNGQSITIEVGKTTGTVTTTAPNDALTGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATNSVAEGGSIIYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVPSTVPAVTQVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGTPVTVTLSNGAVIIIEAGKTTGTVTVAAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLTGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGNVVYTATVGAPVTGSPVTVTLSNGQSITIEVGKTSGTVTTTAPNDVLNGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATNSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVIVNLANGQSITIEVGKTTGTVTTIAPNDALTGQAPLTNSITNVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATIKSATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATTTAAEGGTVTYTATVGAPVTGSPVTVNLANGQSITIEVGKTTGTVTTTAPNDALTGHTPLTNSITNVTGGNYENLVADKTPVSTTVTDTIDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIDAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVSITGSTSVTEGQTASYTVSLTHPAQTEVTLKIVYSGTAADGSDFTGVYTVKIPAGASSAQFNVATIDDKITEGTENFVVKIDSATGGNFENLAVSSTNGSVSTSIIDNDAPPVIDLDANNSSGATGADYKVTFTEGTTGPGVSIADTDIKITDPDSTMLTGATIVLTNRQDGDALNLGNSVNGITINANSTNGTVTLTLSGNATLADYMQAIKNISFTNSSEDPSTVPRIITVTVTDGGNYSNTATTTVNVVAINDAPVAAPVNVTGTEDTPLILGWSTFGVSDVDSPASSLGVKITQLPGEGKLQYLDGSTWKDVANNQTFSKADIDAGKLRFVPDANESGVNGYGGTGVGNNQADYAQIKFQPTDGQLLGNTGTIKIDITPVADAPTLSVADNSVKSTGLIKEVWTGLSGLGTDGSGANSTTLKNVIDGAGKPNTSGTVNNVQSDGSVTAGTASKTSGLIYLEAGKTYTFSGTGDDSLLVTIGGKSVASTTWGAGGNLNGSFTPTTSGYYTLDIYHHNQSGPGSYDVNLSVNGSTPIDLSSAGVPIYTGVQDLVNAGVTVSDLHGSNGEGYYDGYKLNTGAEGTTVKLSSISTALTDTDGSETLSIKISGAPVGSVLSDGAGHSFTVTATSGDANVTGWNLGTLTVTPPTYYNGQFNLTVTSTSTEQVGGSASTTATIPVTVVPAVYNSIVATSADDTVTGTDGNDIMVADIGGLTVVPGTNYNIAFMVDSSGSMSASSINAAKDSLTSVFNTLKQSLGGSNSGTVNIFLVDFDTQVNKSVSVNLNDPNALTLLKAVLDSMASGGGTNYEDVFKATANFFQSAEAVANTGAKNLTYFITDGQPTYYQSNEQTNPTLYGNVKLDDVVKTSNYKLGSSFATYIDSTHYLSISTSGEVVLQTLKNNNWSWNSLGTIHAQGDGTYEISSLAGSGNSTSSNTTDNSAAGFALLSGLSNVEAIGLNNGVSLNDLKPYDSDKTPQTNIDPKDLANSIIGHTEATMPGNDTVSGGDGNDILFGDLVSFNGIAGEGYQAMQAFVAKETGVDVSKVTTSNVHQYITEHYQAFDVSGAHDGNDTLLGGAGNDILFGSGGNDLLDGGKGNDILLGGTGNDTLIGGQGNDILIGGSGADTFVWKAGDTGNDVIKDFKASEGDRIDLRDLLQGETGSTIDNFLKITTVDGVSSLQVSSGGKFNSGDAAAATPDVTIKLEGNNWSSANIHNLIAGSDPTIKVDHNNS lapd-lapf 1339947 not-defined-2024053639 1339949 unspecified role unspecified role prey prey Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-1933455263 1339950 unspecified role unspecified role bait bait q3kk31-- Q3KK31-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 5 lapa-lapf 1339948 n/a 1339951 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain undetermined undetermined sequence position undetermined false not-defined-2024053639 1339949 enzyme enzyme unspecified role unspecified role Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false protein cleavage protein cleavage false false false figure 3