mpidb The Microbial Protein Interaction Database chatterjee-2012-1 Structural characterization of a conserved, calcium-dependent periplasmic protease from Legionella pneumophila. ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Chatterjee D., Boyd C.D., O'Toole G.A., Sondermann H. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation chatterjee-2012-2 Structural characterization of a conserved, calcium-dependent periplasmic protease from Legionella pneumophila. ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chatterjee D., Boyd C.D., O'Toole G.A., Sondermann H. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG q5zxa3_legph lpg0829 protein protein legph Legionella pneumophila subsp. pneumophila MTLTKKMAVGVLIMLLFVFIGTYFITMNNARNFFIQQLESNAQDTATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVQDIKGKVIILKKQLPQESDIPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFALVALVLSYGFLKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTKVKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVIDGLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFEKECQEFEQILSQGINDIKICKIHMGAASYFLHQPVSNLLSTVDQAVKKARETGVFYCQKEHDTYKYPQLISGDEIRNSLQQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGAGYFIPVAEKLGLAYPIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLEDTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDLNIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHIPWGQGRFLSSVELIK q5zxa4_legph lpg0828 protein protein legph Legionella pneumophila subsp. pneumophila MLKESWLLSKSRPRRLSVIAKRFQKVFLESLRYRLVLSIWTGLILCYVTSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWGNLIDSLKKKPVKIQLEKVNSFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRITYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLAKTRGESSLMGDSKSLGKWDALMKRME lapf-lapd 1339867 not-defined-88924066 1339869 unspecified role unspecified role bait bait q3kk32-- Q3KK32-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-944179849 1339870 unspecified role unspecified role prey prey Q3KK31:p.Trp125Glu Q3KK31-125-125 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 lapf-q5zxa3 1339867 not-defined-776411543 1339869 unspecified role unspecified role bait bait q3kk32-- Q3KK32-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-2072476750 1339871 unspecified role unspecified role prey prey Q5ZXA3:p.Trp126Glu Q5ZXA3-126-126 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 q5zxa4-lapd 1339867 not-defined-1412014193 1339870 unspecified role unspecified role prey prey Q3KK31:p.Trp125Glu Q3KK31-125-125 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-624403667 1339872 unspecified role unspecified role bait bait q5zxa4-- Q5ZXA4-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 2 q5zxa4-q5zxa3 1339867 not-defined-915833545 1339872 unspecified role unspecified role bait bait q5zxa4-- Q5ZXA4-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1496891226 1339871 unspecified role unspecified role prey prey Q5ZXA3:p.Trp126Glu Q5ZXA3-126-126 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 q5zxa4-1 1339868 n/a 1339872 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 3 1.645 A (4FGQ) Rworking = 0.19 Rfree = 0.22 (4FGQ)