IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ okuno-2012-5 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-1 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Although this assay was described in a previous paper (PMID:17088211), this specific interaction was not included in the data then published. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp A random-primed cDNA library prepared from rat seizure-stimulated cerebrum Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-2 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-3 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunostaining immunostaining Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-4 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-6 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-7 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation okuno-2012-8 Inverse Synaptic Tagging of Inactive Synapses via Dynamic Interaction of Arc/Arg3.1 with CaMKIIβ. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Okuno H., Akashi K., Ishii Y., Yagishita-Kyo N., Suzuki K., Nonaka M., Kawashima T., Fujii H., Takemoto-Kimura S., Abe M., Natsume R., Chowdhury S., Sakimura K., Worley P.F., Bito H. Brain synaptosomal fractions. Origin of Arc confirmed by the author okuno@m.u-tokyo.ac.jp, hbito@m.u-tokyo.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation kcc2b_rat Calcium/calmodulin-dependent protein kinase type II subunit beta Camk2b protein protein rat Rattus norvegicus (Rat) Rat MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPPAALEPQTTVIHNPVDGIKESSDSTNTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRPDGKWQNVHFHCSGAPVAPLQ arc_rat Activity-regulated cytoskeleton-associated protein Arc Activity-regulated gene 3.1 protein protein protein rat Rattus norvegicus (Rat) Rat MELDHMTTGGLHAYPAPRGGPAAKPNVILQIGKCRAEMLEHVRRTHRHLLTEVSKQVERELKGLHRSVGKLENNLDGYVPTGDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESMGGKYPVGSEPARHTVSVGVGGPEPYCQEADGYDYTVSPYAITPPPAAGELPEQESVGAQQYQSWVPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRKRDLYQTLYVDAEEEEIIQYVVGTLQPKFKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAEPSVTPLPTEDETEALTPALTSESVASDRTQPE kcc2a_rat Calcium/calmodulin-dependent protein kinase type II subunit alpha Camk2a protein protein rat Rattus norvegicus (Rat) Rat MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH kcc2b_mouse Calcium/calmodulin-dependent protein kinase type II subunit beta Camk2b Camk2d protein protein mouse Mus musculus Mouse MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPPAALEPQTTVIHNPVDGIKESSDSTNTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ arc_mouse Activity-regulated cytoskeleton-associated protein Arc Activity-regulated gene 3.1 protein protein protein mouse Mus musculus Mouse MELDHMTTGGLHAYPAPRGGPAAKPNVILQIGKCRAEMLEHVRRTHRHLLTEVSKQVERELKGLHRSVGKLENNLDGYVPTGDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESMGGKYPVGSEPARHTVSVGVGGPEPYCQEADGYDYTVSPYAITPPPAAGELPEQESVEAQQYQSWGPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELELPQKQGEPLDQFLWRKRDLYQTLYVDAEEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAEPSGTPLPTEDETEALTPALTSESVASDRTQPE arc-camk2b-3 1299314 n/a 1299322 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection P08413:p.Thr287Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. n/a 1299323 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1299314 Fig. 1C, 1D, 6B, C, D, Supp. Fig. 1F This interaction was strong in the absence of Ca2+ and calmodulin (CaM) but was attenuated when Ca2+/CaM was present. Inhibition of CaM activation by W-7 (100 uM) in the presence of Ca2+/CaM restored Arc binding to CaMKIIB. arc-camk2a 1299314 n/a 1299324 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. n/a 1299323 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1299314 Fig. 1D, Supp. Figs. 1E, 1F interaction was observed only in the presence of Ca2+/CaM. arc-camk2b 1299315 n/a 1299322 unspecified role unspecified role prey prey n/a 1299323 unspecified role unspecified role bait bait physical association physical association false false false arc-camk2b-1 1299316 n/a 1299323 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n/a 1299322 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association false false false Supp. Fig. 1A arc-camk2b-2 1299317 n/a 1299322 unspecified role unspecified role neutral component neutral component n/a 1299323 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 1A camk2b-arc 1299318 n/a 1299325 unspecified role unspecified role bait bait n/a 1299326 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B, Supp. Fig. 7B arc-camk2b-5 1299319 n/a 1299323 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1299322 unspecified role unspecified role fluorescence donor fluorescence donor region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Supp. Fig. 1C camk2b-arc-4 1299320 n/a 1299325 unspecified role unspecified role bait bait n/a 1299326 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. Fig. 7B camk2b-arc-3 1299321 n/a 1299322 unspecified role unspecified role bait bait n/a 1299323 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B