UniProt UniProt http://www.uniprot.org o_brien-2012-1 Interaction of voltage-gated sodium channel Nav1.6 (SCN8A) with microtubule-associated protein Map1b. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined O'Brien J.E., Sharkey L.M., Vallianatos C.N., Han C., Blossom J.C., Yu T., Waxman S.G., Dib-Hajj S.D., Meisler M.H. meislerm@umich.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation o_brien-2012-2 Interaction of voltage-gated sodium channel Nav1.6 (SCN8A) with microtubule-associated protein Map1b. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western O'Brien J.E., Sharkey L.M., Vallianatos C.N., Han C., Blossom J.C., Yu T., Waxman S.G., Dib-Hajj S.D., Meisler M.H. meislerm@umich.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation o_brien-2012-3 Interaction of voltage-gated sodium channel Nav1.6 (SCN8A) with microtubule-associated protein Map1b. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip monoclonal western monoclonal antibody western blot monoclonal western O'Brien J.E., Sharkey L.M., Vallianatos C.N., Han C., Blossom J.C., Yu T., Waxman S.G., Dib-Hajj S.D., Meisler M.H. meislerm@umich.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation o_brien-2012-4 Interaction of voltage-gated sodium channel Nav1.6 (SCN8A) with microtubule-associated protein Map1b. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence O'Brien J.E., Sharkey L.M., Vallianatos C.N., Han C., Blossom J.C., Yu T., Waxman S.G., Dib-Hajj S.D., Meisler M.H. meislerm@umich.edu Mouse brain cDNA J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation scn8a_mouse Sodium channel protein type 8 subunit alpha Scn8a Nbna1 Sodium channel protein type VIII subunit alpha Voltage-gated sodium channel subunit alpha Nav1.6 protein protein mouse Mus musculus Mouse MAARVLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVVWPINFNESYLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAGQCPEGFQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQCSRSSRIFPSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEATTEVEIKKKGPGSLLVSMEQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISQECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWAKVKVHAFMQAHFKQREADEVKPLDELYEKKANCIANHTGVDIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIDEVNNKTDCEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICRKITSNKLENGGTHREKKESTPSTASLPSYDSVTKPDKEKQQRAEEGRRERAKRQKEVRESKC map1b_mouse Microtubule-associated protein 1B MAP1.2 MAP1(X) Map1b Mtap1b Mtap5 protein protein mouse Mus musculus Mouse MATVVVEATEPEPSGSIGNPAASTSPSLSHRFLDSKFYLLVVVGETVTEEHLRRAIGNIELGIRSWDTNLIECNLDQELKLFVSRHSARFSPEVPGQKILHHRSDVLETVVLINPSDEAVSTEVRLMITDAARHKLLVLTGQCFENTGELILQSGSFSFQNFIEIFTDQEIGELLSTTHPANKASLTLFCPEEGDWKNSNLDRHNLQDFINIKLNSASILPEMEGLSEFTEYLSESVEVPSPFDILEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGFNMLINGGSERKSCFWKLIRHLDRVDSILLTHIGDDNLPGINSMLQRKIAELEEERSQGSTSNSDWMKNLISPDLGVVFLNVPENLKDPEPNIKMKRSIEEACFTLQYLNKLSMKPEPLFRSVGNTIEPVILFQKMGVGKLEMYVLNPVKSSKEMQYFMQQWTGTNKDKAELILPNGQEVDIPISYLTSVSSLIVWHPANPAEKIIRVLFPGNSTQYNILEGLEKLKHLDFLKQPLATQKDLTGQVPTPPVKQVKLKQRADSRESLKPATKPVASKSVRKESKEETPEVTKTSQVEKTPKVESKEKVLVKKDKPVKTESKPSVTEKEVSSKEEQSPVKAEVAEKQATESKPKVTKDKVVKKEIKTKLEEKKEEKPKKEVVKKEDKTPLKKDEKPRKEEVKKEIKKEIKKEERKELKKEVKKETPLKDAKKEVKKEEKKEVKKEEKEPKKEIKKISKDIKKSTPLSDTKKPSALKPKVAKKEESTKKEPLAAGKLKDKGKVKVIKKEGKTTEAAATAVGTAATTAAVVAAAGIAASGPVKELEAERSLMSSPEDLTKDFEELKAEEIDVAKDIKPQLELIEDEEKLKETQPGEAYVIQKETEVSKGSAESPDEGITTTEGEGECEQTPEELEPVEKQGVDDIEKFEDEGAGFEESSETGDYEEKAETEEAEEPEEDGEDNASGSASKHSPTEDDESAKAEADVHLKEKRESVVSGDDRAEEDMDDVLEKGEAEQSEEEGEEEDKAEDAREEGYEPDKTEAEDYVMAVADKAAEAGVTEEQYGYLGTSAKQPGIQSPSREPASSIHDETLPGGSESEATASDEENREDQPEEFTATSGYTQSTIEISSEPTPMDEMSTPRDVMSDETNNEETESPSQEFVNITKYESSLYSQEYSKPAVASFNGLSEGSKTDATDGKDYNASASTISPPSSMEEDKFSKSALRDAYCSEEKELKASAELDIKDVSDERLSPAKSPSLSPSPPSPIEKTPLGERSVNFSLTPNEIKVSAEGEARSVSPGVTQAVVEEHCASPEEKTLEVVSPSQSVTGSAGHTPYYQSPTDEKSSHLPTEVTEKPQAVPVSFEFSEAKDENERASLSPMDEPVPDSESPVEKVLSPLRSPPLLGSESPYEDFLSADSKVLGRRSESPFEGKNGKQGFPDRESPVSDLTSTGLYQDKQEEKSTGFIPIKEDFGPEKKTSDVETMSSQSALALDERKLGGDVSPTQIDVSQFGSFKEDTKMSISEGTVSDKSATPVDEGVAEDTYSHMEGVASVSTASVATSSFPEPTTDDVSPSLHAEVGSPHSTEVDDSLSVSVVQTPTTFQETEMSPSKEECPRPMSISPPDFSPKTAKSRTPVQDHRSEQSSMSIEFGQESPEHSLAMDFSRQSPDHPTLGASVLHITENGPTEVDYSPSDIQDSSLSHKIPPTEEPSYTQDNDLSELISVSQVEASPSTSSAHTPSQIASPLQEDTLSDVVPPREMSLYASLASEKVQSLEGEKLSPKSDISPLTPRESSPLYSPGFSDSTSAAKETAAAHQASSSPPIDAATAEPYGFRSSMLFDTMQHHLALNRDLTTSSVEKDSGGKTPGDFNYAYQKPENAAGSPDEEDYDYESQEKTIRTHDVGGYYYEKTERTIKSPCDSGYSYETIEKTIKTPEDGGYTCEITEKTTRTPEEGGYSYEISEKTTRTPEVSGYTYEKTERSRRLLDDISNGYDDTEDGGHTLGDCSYSYETTEKITSFPESESYSYETSTKTTRSPDTSAYCYETMEKITKTPQASTYSYETSDRCYTTEKKSPSEARQDVDLCLVSSCEFKHPKTELSPSFINPNPLEWFAGEEPTEESEKPLTQSGGAPPPSGGKQQGRQCDETPPTSVSESAPSQTDSDVPPETEECPSITADANIDSEDESETIPTDKTVTYKHMDPPPAPMQDRSPSPRHPDVSMVDPDALAVDQNLGKALKKDLKEKTKTKKPGTKTKSSSPVKKGDGKSKPLAASPKPGALKESSDKVSRVASPKKKESVEKATKTTTTPEVKATRGEEKDKETKNAANASASKSAKTATAGPGTTKTAKSSTVPPGLPVYLDLCYIPNHSNSKNVDVEFFKRVRSSYYVVSGNDPAAEEPSRAVLDALLEGKAQWGSNMQVTLIPTHDSEVMREWYQETHEKQQDLNIMVLASSSTVVMQDESFPACKIEL scn8a-map1b 1330810 n/a 1330814 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1330815 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 2 scn8a-map1b-3 1330810 n/a 1330814 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1330815 unspecified role unspecified role prey prey physical association physical association false false false Figure 4a map1b-scn8a-2 1330810 n/a 1330814 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1330815 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Figure 5a scn8a-map1b-1 1330811 n/a 1330815 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1330814 unspecified role unspecified role bait bait region fusion protein fusion protein monoclonal western monoclonal antibody western blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 3 map1b-scn8a 1330811 n/a 1330815 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1330814 unspecified role unspecified role prey prey region fusion protein fusion protein monoclonal western monoclonal antibody western blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 3 scn8a-map1b-2 1330812 n/a 1330814 unspecified role unspecified role bait bait n/a 1330815 unspecified role unspecified role prey prey physical association physical association false false false Figure 3b scn8a-map1b-4 1330813 n/a 1330814 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330815 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1330847 1330845 physical association physical association false false false Fig. 2A