IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ katayama-2012-21 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-3 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer synchronized mitotic cells Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-2 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-6 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-9 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-15 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-19 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-20 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-22 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-1 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-4 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer The EMSA probe is stated to be a "p21 (CDKN1A) containing the p53 DNA-binding site", but it has not been possible to confirm this. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-5 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer glutaraldehyde for 15 min Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-7 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-8 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-10 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer mortalin was detected using an anti-mortalin antibody. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-11 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-12 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-panc1 Homo sapiens pancreatic carcinoma cell line PANC-1 panc1 Human pancreatic carcinoma cell line PANC-1 Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-13 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-14 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-panc1 Homo sapiens pancreatic carcinoma cell line PANC-1 panc1 Human pancreatic carcinoma cell line PANC-1 Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer GFP tagged proteins were detected by tag fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-16 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Mad2 was detected by Western Blotting. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-17 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer GFP tagged proteins were detected by tag-fluorescence. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-18 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. katayama-2012-23 Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Katayama H., Wang J., Treekitkarnmongkol W., Kawai H., Sasai K., Zhang H., Wang H., Adams H.P., Jiang S., Chakraborty S.N., Suzuki F., Arlinghaus R.B., Liu J., Mobley J.A., Grizzle W.E., Wang H., Sen S. Cancer - Interactions investigated in the context of cancer Mortalin was detected using an anti-Flag antibody. Figures 1B,1G, 5J, 6C, and S5B have not been annotated as the antibody used is not Aurora--A specific. ssen@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation The authors confirmed that all constructs/proteins used were of human origin. p73_human Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein human Homo sapiens Human MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH p73_chlae Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MAQSTTTSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSAPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGPGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAATPNLGPVGSGMLNNHGHAVPANSEMTSSHGTQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYGAAAQQLLRSSNAAAISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGAGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH aurka_chlae AURKA_CHLAE protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey grp75_chlae HSPA9/Mortalin protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS grp75_human Stress-70 protein, mitochondrial HSPA9 GRP75 HSPA9B 75 kDa glucose-regulated protein Heat shock 70 kDa protein 9 Peptide-binding protein 74 Mortalin mt-HSP70 protein protein human Homo sapiens Human MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD md2l1_human Mitotic spindle assembly checkpoint protein MAD2A MAD2L1 MAD2 Mitotic arrest deficient 2-like protein 1 protein protein human Homo sapiens Human MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR p53/p73_binding _consensus_site_probe probe containing the p53 DNA-binding site probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) TACAGAACATGTCTAAGCATGCTGGGG o15350-8 Tumor protein p73 TP73 P73 p53-like transcription factor p53-related protein protein protein human Homo sapiens Human MLYVGDPARHLATAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH Produced by alternative promoter usage. tp73-tp73-1 1272344 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1272367 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S3A -LHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. tp73-1 1272344 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 4.0 physical association physical association false false false S3A -LHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. tp73-3 1272344 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 physical association physical association false false false S3A -LHS mmm Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. tp73-tp73-2 1272344 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S3A LHS aurka-tp73-2 1272345 n/a 1272368 unspecified role unspecified role bait bait n/a 1272369 unspecified role unspecified role prey prey physical association physical association false false false Fig 1I,1J,1K. The amount of Aurora-A bound to p73 progressively increased, peaking at mitosis. tp73-grp75-2 1272345 n/a 1272370 unspecified role unspecified role prey prey n/a 1272368 unspecified role unspecified role bait bait physical association physical association false false false Fig 4D tp73-grp75-1 1272345 n/a 1272370 unspecified role unspecified role prey prey n/a 1272368 unspecified role unspecified role bait bait physical association physical association false false false Fig 4E aurka-tp73-1 1272346 n/a 1272371 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 1H aurka-tp73-7 1272346 n/a 1272371 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig S1C aurka-tp73-3 1272347 n/a 1272368 unspecified role unspecified role neutral component neutral component n/a 1272371 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 3E, 3F. Cells overexpressing Aurora-A showed evenly diffused endogenous p73 staining in the cytoplasm and nucleus, which were reversed with Aurora-A inhibitor MLN8054. aurka-tp73-4 1272347 n/a 1272372 unspecified role unspecified role neutral component neutral component p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig 5A/B In cells with mutant mortalin, the p73 S235D mutant translocated into the nucleus more than in the empty-vector-transfected cells tp73-hspa9-2 1272348 n/a 1272372 unspecified role unspecified role bait bait p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 4C WT and mutant p73 did not interact with the mortalin deletion mutant, but full-length mortalin's interaction was enhanced with S235D. hspa9-tp53-1 1272348 n/a 1272372 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1272373 unspecified role unspecified role prey prey P04637:p.Ser215Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig S3B WT and mutant p53 did not interact with the mortalin deletion mutant, but full-length mortalin's interaction was enhanced with S215D. tp73-aurka-4 1272349 n/a 1272374 unspecified role unspecified role prey prey n/a 1272371 unspecified role unspecified role prey prey n/a 1272367 unspecified role unspecified role bait bait n/a 1272375 unspecified role unspecified role prey prey association association false false false Fig 7E tp73-aurka-5 1272349 n/a 1272367 unspecified role unspecified role prey prey n/a 1272375 unspecified role unspecified role bait bait n/a 1272371 unspecified role unspecified role prey prey n/a 1272374 unspecified role unspecified role prey prey association association false false false Fig 7E aurka-tp73-8 1272350 n/a 1272367 unspecified role unspecified role prey prey n/a 1272371 unspecified role unspecified role bait bait physical association physical association false false false Fig S1D aurka-tp73-9 1272350 n/a 1272367 unspecified role unspecified role bait bait n/a 1272371 unspecified role unspecified role prey prey physical association physical association false false false Fig S1D aurka-tp73-10 1272351 n/a 1272371 unspecified role unspecified role prey prey n/a 1272367 unspecified role unspecified role bait bait physical association physical association false false false Fig S1E aurka-tp73-10 1272351 n/a 1272371 unspecified role unspecified role bait bait n/a 1272367 unspecified role unspecified role prey prey physical association physical association false false false Fig S1E hspa9-tp73 1272352 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272372 unspecified role unspecified role neutral component neutral component physical association physical association false false false S3A -RHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. hspa9-tp73-1 1272352 n/a 1272367 unspecified role unspecified role neutral component neutral component O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272372 unspecified role unspecified role neutral component neutral component physical association physical association false false false S3A -RHS Enrichment of S235D mutant and mortalin in the higher molecular complex was also evident. aurka-tp73 1272353 n/a 1272371 enzyme enzyme neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O14965:p.Lys162Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false n/a 1272367 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false false false Fig 1E, 1F, S1A p73 is phosphorylated by Aurora-A. The N terminally truncated isoform Delta Np73 showed minimal loss of phosphorylation in the SA mutant. p53-aurka 1272354 n/a 1272376 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false O15350:p.Ser235Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 2A tp73-1 1272355 n/a 1272367 putative self putative self putative self putative self region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 4.0 physical association physical association false false false Fig 3D tp73-hspa9 1272356 n/a 1272367 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1272372 unspecified role unspecified role prey prey physical association physical association false false false Fig 4A A protein band of approximately 80 kD MW was detected only in the immune complex of the S235D mutant but not the WT. tp73-hspa9-1 1272357 n/a 1272367 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1272372 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 4B The S235D mutant and mortalin-containing complexes were significantly more enriched at >2-megadalton-sized fractions than were the p73-WT and mortalin complexes. tp73-aurka-9 1272358 n/a 1272371 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272368 unspecified role unspecified role bait bait n/a 1272370 unspecified role unspecified role prey prey association association false false false Fig 4E Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 tp73-aurka-1 1272359 n/a 1272372 unspecified role unspecified role prey prey n/a 1272367 unspecified role unspecified role bait bait n/a 1272371 unspecified role unspecified role prey prey association association false false false Fig 4F Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 tp73-aurka-2 1272360 n/a 1272367 unspecified role unspecified role bait bait n/a 1272372 unspecified role unspecified role prey prey n/a 1272371 unspecified role unspecified role prey prey association association false false false Fig 4F Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054 hspa9-tp73-2 1272361 n/a 1272372 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1272367 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 5F Nuclear staining was detected in 36% of mortalin mutant MCF-7 and Panc-1 cells (n = 100) versus 2% of empty vector cells. hspa9-tp73-3 1272362 n/a 1272367 unspecified role unspecified role neutral component neutral component n/a 1272372 unspecified role unspecified role neutral component neutral component p53_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig 5F Nuclear staining was detected in 36% of mortalin mutant MCF-7 and Panc-1 cells (n = 100) versus 2% of empty vector cells. tp73-aurka-6 1272363 n/a 1272367 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O15350:p.Ser235Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1272374 unspecified role unspecified role prey prey n/a 1272375 unspecified role unspecified role bait bait association association false false false Fig 7F There was a marked reduction in the interaction of both S235D mutant and MAD2 with CDC20, compared with that in empty vector and S235A mutant cells. tp73-aurka-7 1272364 n/a 1272367 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272374 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 7G tp73-tp73-3 1272365 n/a 1272377 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1272367 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S1B Isoform Delta Np73 appeared to bind the WT and the phosphor-mimetic (S235D) mutant of p73 with similar efficiency. tp73-aurka-8 1272366 n/a 1272371 unspecified role unspecified role prey prey n/a 1272372 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false p53_binding_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1272367 unspecified role unspecified role bait bait association association false false false Fig S3C Less mortalin bound to p73 in Aurora-A inhibitor MLN8054 treated cells, than in untreated cells. MLN8054