UniProt UniProt http://www.uniprot.org pratelli-2012-1 The ubiquitin E3 ligase LOSS OF GDU2 is required for GLUTAMINE DUMPER1-induced amino acid secretion in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Pratelli R., Guerra D.D., Yu S., Wogulis M., Kraft E., Frommer W.B., Callis J., Pilot G. gpilot@vt.edu Plant Physiol. (0032-0889) 2012 Only protein-protein interactions imex curation pratelli-2012-3 The ubiquitin E3 ligase LOSS OF GDU2 is required for GLUTAMINE DUMPER1-induced amino acid secretion in Arabidopsis. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Pratelli R., Guerra D.D., Yu S., Wogulis M., Kraft E., Frommer W.B., Callis J., Pilot G. gpilot@vt.edu Plant Physiol. (0032-0889) 2012 Only protein-protein interactions imex curation pratelli-2012-4 The ubiquitin E3 ligase LOSS OF GDU2 is required for GLUTAMINE DUMPER1-induced amino acid secretion in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Pratelli R., Guerra D.D., Yu S., Wogulis M., Kraft E., Frommer W.B., Callis J., Pilot G. gpilot@vt.edu Arabidopsis cell cDNA Plant Physiol. (0032-0889) 2012 Only protein-protein interactions imex curation pratelli-2012-2 The ubiquitin E3 ligase LOSS OF GDU2 is required for GLUTAMINE DUMPER1-induced amino acid secretion in Arabidopsis. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Pratelli R., Guerra D.D., Yu S., Wogulis M., Kraft E., Frommer W.B., Callis J., Pilot G. gpilot@vt.edu Plant Physiol. (0032-0889) 2012 Only protein-protein interactions imex curation gdu1_arath Protein GLUTAMINE DUMPER 1 GDU1 F28M20.80 At4g31730 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRPLSVQSKFEDVATSTSVNHHGVTPQSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSSSGEEDGQNVDEEKESRSGDKAANGAYEEKFLVIMAGEDLPRYLATPAMKKCTCGGHEGKMVISQEESVAKEEEKMREGEEEKVKDTGETTTTSH lofg2_arath Probable E3 ubiquitin-protein ligase LOG2 LOG2 Protein LOSS OF GDU2 RF215 RIG2 RING finger protein 215 F11F8.36 F8A24.18 At3g09770 Probable RING-type E3 ubiquitin transferase LOG2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNISSSGGEGRRRRRRNHTAAPPPPPPPPSSSLPPPPLPTEIQANPIVFAAVTPYPNPNPNPVYQYPASYYHHPPPGAMPLPPYDHHLQHHPPHPYHNHSWAPVAMARYPYAGHMMAQPTPYVEHQKAVTIRNDVNLKKESLRLEPDPDNPGRFLVSFTFDATVSGRISVIFFAKESEDCKLTATKEDILPPITLDFEKGLGQKFKQSSGSGIDFSVFEDVELFKAAADTEIYPLAVKAEAAPSGGENEEEERSGSKNAQITQAVYEKDKGEIKIRVVKQILWVNGTRYELQEIYGIGNTVEGDDDSADDANDPGKECVICLSEPRDTTVLPCRHMCMCSGCAKVLRFQTNRCPICRQPVERLLEIKVHGNNGSGNNTGQGETVEQE lul1_arath Probable E3 ubiquitin-protein ligase LUL1 LUL1 Protein LOG2-LIKE UBIQUITIN LIGASE 1 RF370 RING finger protein 370 F15A17_230 MOK16.11 At5g03200 Probable RING-type E3 ubiquitin transferase LUL1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNLISLIFCCGRRQRSNIPPAMETAPLELPPNRFVFAAVPPYLNPNPNYVDQYPGNCLPPPVTEPPMLPYNFNHLHHYPPNSYQLPHPLFHGGRYPILPPPTYVHQKAVTIRNDVNLKKKTLTLIPDPENPNRLLVSFTFDASMPGRITVVFFATEDAECNLRATKEDTLPPITFDFGEGLGQKFIQSSGTGIDLTAFKDSELFKEVDTDVFPLAVKAEATPAEEGKSGSTNVQITQVVYTKEKGEIKIEVVKQILWVNKRRYELLEIYGIENTVDGSDEGKECVVCLSEPRDTTVLPCRHMCMCSGCAKALRFQTNLCPVCRQPVEMLLEINKNG gdu2_arath Protein GLUTAMINE DUMPER 2 GDU2 F14M19.40 At4g25760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQTMEGRQYNYQDSINASSSMVVPHSPWHSPVPYLFGGLAAMLALICVALLILACSYWRLSGSAERDLEAGDDAKPDNDTNKTKHTEMPEKFLVIMAGDVRPTYLATPATRSEQSCTCGDHNEEEGRRG gdu4_arath Protein GLUTAMINE DUMPER 4 GDU4 F27A10.11 At2g24762 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRPLSIKPTSLDVARHATSVESFGNHRPPISPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSTSGDDSGERVDEEKESRSGVKAASAACEEKVLVIMAGDDLPRFLATPAANKCMCGHEGRMVIFKEDGIGAGEEKMGDREKAKENEETTSQ gdu3_arath Protein GLUTAMINE DUMPER 3 GDU3 LBS1 Protein LESS SUSCEPTIBLE TO BSCTV 1 MRI1.4 At5g57685 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGRQYYPPRENVEGNRTTMGGGPHSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSGYLDGEENQSRERDLEVGDVKPDKTAVKPVALPEKFLVIMAGNVKPTYLATPSVKTCTCDDDDDEDDDVEGSDQVVPRSSESNGETH gdu5_arath Protein GLUTAMINE DUMPER 5 GDU5 F6A4.130 At5g24920 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRQFPSIRGNINEKMMTTMVESQTRSPWRTPVPYLFGGLAAMLGLIAFALLLLACSYWRLSRQTEDEEKQTESGEKVVAKAFEEKILVIMAGQNNPTFLATPVAAKICLDCVNMEKKEGQNGESKVTEENH gdu6_arath Protein GLUTAMINE DUMPER 6 GDU6 T4A2.7 At3g30725 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRPTPKVEIWKSPVPYLFGGLFLLVLLIALALLSLVCTHQKPSSSSNNNHMDEEDDVGDKDAKPITREYLPKIVVILAGDNKPTCLAVPVVVPPPTSIFRCNCDNVTVIST gdu7_arath Protein GLUTAMINE DUMPER 7 GDU7 MKD10.70 At5g38770 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLHRDSMVPVNSRLENMDSPILSKICAWGVMLGLFALSLFAMAYACYHKQTSNSCIEEKQGKKQVLKPLDMEPKIVVIMAGNENPTFFAKPTQINA lul2_arath Probable E3 ubiquitin-protein ligase LUL2 LUL2 Protein LOG2-LIKE UBIQUITIN LIGASE 2 RF269 RING finger protein 269 F4P12_110 At3g53410 Probable RING-type E3 ubiquitin transferase LUL2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNVISGGSRPENHRDRTSPPYPNPNAQYQGNYPSPYQDCARYPYGEMASPVQYVEHQEAVTIRNDINLKKETLRLEPDEQNPGKFLLSFTFDASVPGSITVMFFAKEGKDCNLIATKEDLFPSTQVSFAKGLEQRFKQACGTGIDFSDMSEADLVEANETDVYHVAVKAEVVSEDDHPESGTPNRQITHVVLEKDHKGEYKARVVKQILWVNGNRYVLQEIYGIGNTVDDNGEDANERGKECVICLSEPRDTTVLPCRHMCMCSGCAKLLRFQTNLCPICRQPVDRLLEITVNNNDRN lul3_arath Probable E3 ubiquitin-protein ligase LUL3 Probable RING-type E3 ubiquitin transferase LUL3 LUL3 Protein LOG2-LIKE UBIQUITIN LIGASE 3 RF398 RING finger protein 398 T16G12.120 At5g19080 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGISLSKRRRDNNNNHHHPHHNPPYYYSDPPPQQPPPQNGYSYSHNYPVSTPQLSLPPPPAQPPSSSQPPPSQISYRPYGQNYHQNQYYPQQAPPYFTGYHHNGFNPMMRPVYFGPTPVAVMEPPAPYVEHQTAKKVKNDVNVNKATVRLVADDLNPGHYLVSFVFDALFDGSFTIIFFGEEESKCTIVPHLPEAFPPIKVPFQKGAGQKFLQAPGTGIDLGFFSLDDLSKPSPEEVYPLVISAETVISPSSVSEEPLVHKQITQAVLEKTNDGSFKVKVMKQILWIEGERYELQELYGIDNSITQGTAASGLEDTGGKECVICLTEPKDTAVMPCRHLCLCSDCAEELRFQTNKCPICRQPIHELVKIKVESSDEQH log2-gdu1 1317708 n/a 1317712 unspecified role unspecified role prey prey O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A, Fig. 8D gdu1-log2 1317708 n/a 1317712 unspecified role unspecified role bait bait O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1317713 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A, Fig. 8D lul1-gdu1 1317708 n/a 1317714 unspecified role unspecified role bait bait n/a 1317712 unspecified role unspecified role prey prey O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A log2-gdu2 1317708 n/a 1317715 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A gdu2-log2 1317708 n/a 1317713 unspecified role unspecified role prey prey n/a 1317715 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A log2-gdu4 1317708 n/a 1317716 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A gdu3-log2 1317708 n/a 1317717 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A gdu4-log2 1317708 n/a 1317713 unspecified role unspecified role prey prey n/a 1317716 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A log2-gdu3 1317708 n/a 1317717 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A log2-gdu5 1317708 n/a 1317718 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A log2-gdu6 1317708 n/a 1317719 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A gdu6-log2 1317708 n/a 1317719 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A log2-gdu7 1317708 n/a 1317720 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317713 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A lul1-gdu2 1317708 n/a 1317715 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317714 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A lul1-gdu3 1317708 n/a 1317717 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317714 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A lul1-gdu4 1317708 n/a 1317716 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317714 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A lul1-gdu5 1317708 n/a 1317714 unspecified role unspecified role bait bait n/a 1317718 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A lul1-gdu6 1317708 n/a 1317719 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317714 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A gdu6-lul1 1317708 n/a 1317719 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317714 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A lul2-gdu2 1317708 n/a 1317721 unspecified role unspecified role bait bait n/a 1317715 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A gdu2-lul2 1317708 n/a 1317715 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317721 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A lul2-gdu3 1317708 n/a 1317717 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1317721 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A gdu3-lul2 1317708 n/a 1317721 unspecified role unspecified role prey prey n/a 1317717 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A lul3-gdu2 1317708 n/a 1317722 unspecified role unspecified role bait bait n/a 1317715 unspecified role unspecified role prey prey cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Fig. 1A gdu1_log2-1 1317709 n/a 1317713 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9S752:p.[Cys354Ala;Cys357Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1317712 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1C log2-gdu1-1 1317709 n/a 1317713 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9S752:p.[Cys354Ala;Cys357Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1317712 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1C, Fig. 4G log2-gdu1-2 1317709 n/a 1317713 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9S752:p.[Gly2Ala;Cys354Ala;Cys357Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1317712 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4G log2-gdu1 1317709 n/a 1317712 unspecified role unspecified role prey prey O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1317713 unspecified role unspecified role bait bait Q9S752:p.[Cys354Ala;Cys357Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1C gdu1-log2 1317709 n/a 1317713 unspecified role unspecified role prey prey Q9S752:p.[Cys354Ala;Cys357Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1317712 unspecified role unspecified role bait bait O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1C gdu1-log2 1317710 n/a 1317712 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1317713 unspecified role unspecified role prey prey physical association physical association false false false gdu1-lul1 1317710 n/a 1317712 unspecified role unspecified role bait bait cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1317714 unspecified role unspecified role prey prey physical association physical association false false false log2-gdu1-4 1317711 n/a 1317712 unspecified role unspecified role prey prey O81775:p.Gly100Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cytoplasmic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1317713 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1B