InnateDB InnateDB gringhuis-2012-3 Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. gringhuis-2012-1 Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl Curdlan (ug/ml) Piceatannol (uM) Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. gringhuis-2012-2 Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl Curdlan (ug/ml) Piceatannol (uM) Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. gringhuis-2012-4 Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1β via a noncanonical caspase-8 inflammasome. human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. Curdlan is a (1,3)-beta-glucan, a PAMP recognised by Dectin-1. Piceatannol is a Syk inhibitor. s.i.ringhuis@amc.uva.nl,t .b.geijtenbeek@amc.uva.nl t.b.geijtenbeek@amc.uva.nl, t.b.geijtenbeek@amc.uva.nl Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B., Gringhuis S.I., Kaptein T.M., Wevers B.A., Theelen B., van der Vlist M., Boekhout T., Geijtenbeek T.B. card9_human Caspase recruitment domain-containing protein 9 CARD9 protein protein human Homo sapiens Human MSDYENDDECWSVLEGFRVTLTSVIDPSRITPYLRQCKVLNPDDEEQVLSDPNLVIRKRKVGVLLDILQRTGHKGYVAFLESLELYYPQLYKKVTGKEPARVFSMIIDASGESGLTQLLMTEVMKLQKKVQDLTALLSSKDDFIKELRVKDSLLRKHQERVQRLKEECEAGSRELKRCKEENYDLAMRLAHQSEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGLSSGEPPEKERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS malt1_human Mucosa-associated lymphoid tissue lymphoma translocation protein 1 MALT lymphoma-associated translocation Paracaspase MALT1 MLT protein protein human Homo sapiens Human MSLLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSELLDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQGTEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPEIIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLVFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSSAATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHFSRSNVPVETTDEIPFSFSDRLRISEK bcl10_human B-cell lymphoma/leukemia 10 BCL10 CIPER CLAP Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B protein protein human Homo sapiens Human MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS card9-bcl10 1308578 n/a 1308582 unspecified role unspecified role bait bait n/a 1308583 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role prey prey n/a 1308585 unspecified role unspecified role prey prey association association false false false Fig. 3C. curdlan CARD9 was not associated with MALT1–caspase-8 or MALT1–Bcl-10 before dectin-1 triggering. However, stimulation with curdlan resulted in CARD9 immunoprecipitating together with MALT1 and Bcl-10 as well as with caspase-8, and this was dependent on Syk activation. bcl10-card9-3 1308578 n/a 1308585 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role prey prey n/a 1308586 unspecified role unspecified role bait bait n/a 1308582 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role prey prey association association false false false Fig. 3I. curdlan ASC immunoprecipitated together with MALT1, caspase-8, CARD9 and Bcl-10 in whole-cell extracts of curdlan-stimulated DCs but not in those of unstimulated DCs. pycard-casp8 1308578 n/a 1308583 unspecified role unspecified role prey prey n/a 1308586 unspecified role unspecified role bait bait n/a 1308585 unspecified role unspecified role prey prey association association false false false Fig. 3K. curdlan After triggering of dectin-1, ASC did not associate with caspase-8 in extracts of cells in which MALT1 was silenced. casp8-malt1 1308578 n/a 1308583 unspecified role unspecified role prey prey n/a 1308586 unspecified role unspecified role prey prey n/a 1308585 unspecified role unspecified role bait bait association association false false false Fig. 3K-2 curdlan casp8-malt1-2 1308578 n/a 1308585 unspecified role unspecified role bait bait n/a 1308583 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3K-3 curdlan ASC did not associate with caspase-8 in extracts of cells in which MALT1 was silenced. bcl10-card9 1308579 n/a 1308582 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role bait bait n/a 1308585 unspecified role unspecified role prey prey n/a 1308586 unspecified role unspecified role prey prey association association false false false Fig. 3A, 3J. curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 10 0 malt1-bcl10 1308579 n/a 1308585 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role bait bait n/a 1308584 unspecified role unspecified role prey prey association association false false false Fig. 3A, 3J -2 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 40 malt1-bcl10-1 1308579 n/a 1308585 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role bait bait n/a 1308584 unspecified role unspecified role prey prey association association false false false Fig. 3A, 3J -3 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 0 malt1-bcl10-2 1308579 n/a 1308585 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role bait bait association association false false false Fig. 3A, 3J -2 curdlan piceatannol Association of CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 10 40 bcl10-card9-1 1308580 n/a 1308583 unspecified role unspecified role prey prey n/a 1308582 unspecified role unspecified role prey prey n/a 1308585 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role bait bait association association false false false Fig. 3B. curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 0 10 bcl10-card9-4 1308580 n/a 1308582 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role bait bait association association false false false Fig. 3B-2 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 0 0 bcl10-card9-5 1308580 n/a 1308584 unspecified role unspecified role bait bait n/a 1308583 unspecified role unspecified role prey prey n/a 1308582 unspecified role unspecified role prey prey association association false false false Fig. 3B-3 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 40 0 bcl10-card9-6 1308580 n/a 1308583 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role bait bait n/a 1308582 unspecified role unspecified role prey prey association association false false false Fig. 3B-3 curdlan piceatannol Association of CARD9 and Casp8 is dependent on curdlan stimulation in the absence of piceatannol. 40 10 bcl10-card9-2 1308581 n/a 1308582 unspecified role unspecified role prey prey n/a 1308584 unspecified role unspecified role prey prey n/a 1308586 unspecified role unspecified role prey prey n/a 1308583 unspecified role unspecified role prey prey n/a 1308585 unspecified role unspecified role bait bait association association false false false Fig. 3D. curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 10 casp8-bcl10 1308581 n/a 1308583 unspecified role unspecified role prey prey n/a 1308585 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3D-2 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 0 0 casp8-malt1-1 1308581 n/a 1308585 unspecified role unspecified role bait bait n/a 1308583 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3D-3 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 40 0 casp8-malt1-3 1308581 n/a 1308585 unspecified role unspecified role bait bait n/a 1308583 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3D-3 curdlan piceatannol Association of Bcl-10, CARD9 and ASC is dependent on curdlan stimulation in the absence of piceatannol. 40 10