mpidb The Microbial Protein Interaction Database galli-2012-1 FtsZ-ZapA-ZapB interactome of Escherichia coli. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Galli E., Gerdes K. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation galli-2012-2 FtsZ-ZapA-ZapB interactome of Escherichia coli. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Galli E., Gerdes K. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation galli-2012-3 FtsZ-ZapA-ZapB interactome of Escherichia coli. in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Galli E., Gerdes K. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation zapb_ecoli Cell division protein ZapB yiiU zapB b3928 JW3899 protein protein ecoli Escherichia coli (strain K12) MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQNAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV zapa_ecoli Cell division protein ZapA ygfE Z ring-associated protein ZapA zapA b2910 JW2878 protein protein ecoli Escherichia coli (strain K12) MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKERTRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITEKTNQNFE ftsz_ecoli Cell division protein FtsZ ftsZ sfiB sulB b0095 JW0093 protein protein ecoli Escherichia coli (strain K12) MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNTDAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAAGMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSLITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMSEMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFETVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQPVMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 zapb-zapb-1 1339326 not-defined-1874929353 1339329 unspecified role unspecified role bait bait not-defined-339870254 1339329 unspecified role unspecified role prey prey p0af36-1-30 P0AF36-1-30 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 3 zapb-zapa 1339326 not-defined-1645724933 1339329 unspecified role unspecified role bait bait P0AF36:p.Lys10_Leu11delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0AF36:p.Ser4_Leu5delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0AF36:p.Phe8_Glu9delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-835167670 1339330 unspecified role unspecified role prey prey region P0ADS2-1-20 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 3 zapa-zapb-1 1339326 not-defined-515910643 1339330 unspecified role unspecified role bait bait not-defined-534290222 1339329 unspecified role unspecified role prey prey region P0AF36-1-10 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 3 zapa-ftsz-3 1339327 not-defined-1887543078 1339330 unspecified role unspecified role bait bait ecolx Escherichia coli not-defined-1602705187 1339331 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false figure 3 zapb-zapa-1 1339327 not-defined-1752504324 1339329 unspecified role unspecified role bait bait p0af36-- P0AF36-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli not-defined-444345505 1339330 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false figure 3C zapa-zapb-2 1339327 not-defined-204425299 1339329 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-1704245596 1339330 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false figure 3 ftsz-gtp-4 1339328 n/a 1339332 enzyme target enzyme target substrate substrate unspecified role unspecified role n/a 1339331 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) gtpase reaction gtpase reaction false false false Fig. 4 ZapB affects FtsZ GTPase activity via ZapA.