matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE http://matrixdb.ibcp.fr lin-2012a-1 Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. in vitro In vitro protease assay protease assay de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med;unc.edu zhiliu@med.unc.edu Matrix Biol. (0945-053X) 2012 Only protein-protein interactions imex curation lin-2012a-2 Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Lin L., Betsuyaku T., Heimbach L., Li N., Rubenstein D., Shapiro S.D., An L., Giudice G.J., Diaz L.A., Senior R.M., Liu Z. zhiliu@med;unc.edu zhiliu@med.unc.edu Matrix Biol. (0945-053X) 2012 Only protein-protein interactions imex curation coha1_mouse Collagen alpha-1(XVII) chain Col17a1 Bp180 Bpag2 180 kDa bullous pemphigoid antigen 2 Bullous pemphigoid antigen 2 protein protein mouse Mus musculus Mouse MDVTKKSKRDGTEVTERIVTETVTTRLTSLPPKGSTSNGYAKTGSLGGGSRLEKQSLTHGSSGYINSSGSIRGNASTSSYRRTHSPASTLPNSPGSTFERKAHMTRHGTYEGSSSGNSSPEYPRKELASSSTRGRSQTRESEIRVRLQSASPSTRWTELDEVKRLLKGSRSASASPTRNTSNTLPIPKKGTVETKTVTASSHSVSGTYDSAILDTNFPPHMWSSTLPAGSSLGTYQNNITAQSTSLLNTNAYSTGSVFGVPNNMASCSPTLHPGLSSCSSVFGMQNNLAPSSSVLSHGTTTASTAYGAKKNVPQPPTVTSTGVSTSATCTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPATISSTSFSEDTLKKEKQAAYAADTCLKADVNGDLNTVSTKSKMTSAENHGYDRGGGGGRGKGGGAGGGGGGGGASGGGGAWGAAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVEELEKTKVLYHDVQMDKSNRDRLQAEAPSLGPGLGKAELDGYSQEAIWLFVRNKLMTEQENGNLRGSPGPKGDMGSQGPKGDRGLPGTPGIPGPLGHPGPEGPKGQKGSIGDPGMEGPIGQRGLAGPMGPRGEPGPPGSGEKGDRGIAGEQGPQGLPGVPGPPGLRGHSGSPGPQGPPGAVGPQGLRGDVGLPGVKGDKGLMGPPGPKGDQGEKGPRGLTGEPGIRGLPGAVGEPGAKGAMGPAGADGQQGSRGEQGLTGMPGTRGPPGPAGDPGKPGLTGPQGPQGLPGSPGRPGTKGEPGAPGRVMTSEGSSTITVPGPPGPPGAMGPPGPPGTPGPAGPAGLPGQQGPRGEPGLAGDSFLSSGSSISEVLSAQGVDLRGPPGPPGPRGPPGPSIPGPPGPRGPPGEGVPGPPGPPGSFLTDSETFFTGPPGPPGPPGPKGDQGDPGVPGTPGISGGLSHGASSSTLYMQGPPGPPGPPGPPGSLSSSGQDIQHYIAEYMQSDNIRTYLSGVQGPPGPPGPPGPVITITGETFDYSQLASQVVSYLRSSGYGAGLSSASSSEDILAMLRRNDVWQYLRQNLVGPPGPPGPPGVSGDGSLLSLDYGELSRHILNYMSSSGISFGHPGPPGPPGLPGTSYEELLTMLRGSDYRNIIGPPGPPGPPGMPGNAWSSISVEDLSSYLHTAGLSSIPGPPGPPGPPGPRGPPGVSAALSTYAAENSDNFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDGHLRENYNWSSNSSARRGTSYSSSTGTGGTNGGSLGEGGAYGAGDGGPYGTDIGPGGGYGAAAGGGIYGTNGDSFRDGFTGDLDYNKLAVRVSESMQRQGLLQGMAYTVQGPPGPQGPPGISRVFSAYSNVTQDLMDFFQTYGTIPGPPGQKGDVGTPGPKGDRGPAGPRGPPGPPGPRGNKGEKGDKGDQVYTGRRKRSIAIKP elne_human Neutrophil elastase Elastase-2 ELANE PMN elastase Bone marrow serine protease Medullasin Human leukocyte elastase ELA2 protein protein human Homo sapiens Human MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH col17a1-elane 1307973 n/a 1307975 enzyme target enzyme target substrate substrate neutral component neutral component tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sequence required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1307976 enzyme enzyme neutral component neutral component human-neutrophil Homo sapiens polymorphonuclear neutrophil neutrophil Neutrophil [Neutrophilic granulocyte] protein cleavage protein cleavage false false false 1A/1C col17a1-elane-7 1307974 n/a 1307975 enzyme target enzyme target substrate substrate neutral component neutral component binding site required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1307976 enzyme enzyme neutral component neutral component human-neutrophil Homo sapiens polymorphonuclear neutrophil neutrophil Neutrophil [Neutrophilic granulocyte] protein cleavage protein cleavage false false false 1B