UniProt UniProt http://www.uniprot.org shih-2012-1 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Shih J.W., Wang W.T., Tsai T.Y., Kuo C.Y., Li H.K., Wu Lee Y.H. Origin of GST-PABP1 construct confirmed by authors (e-mail). tytsai88@gmail.com Biochem. J. (0264-6021) 2012 mimix curation shih-2012-2 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Shih J.W., Wang W.T., Tsai T.Y., Kuo C.Y., Li H.K., Wu Lee Y.H. Origin of GST-PABP1 construct confirmed by authors (e-mail). tytsai88@gmail.com Biochem. J. (0264-6021) 2012 mimix curation shih-2012-4 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Shih J.W., Wang W.T., Tsai T.Y., Kuo C.Y., Li H.K., Wu Lee Y.H. Origin of GST-PABP1 construct confirmed by authors (e-mail). tytsai88@gmail.com Biochem. J. (0264-6021) 2012 mimix curation shih-2012-3 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shih J.W., Wang W.T., Tsai T.Y., Kuo C.Y., Li H.K., Wu Lee Y.H. Origin of GST-PABP1 construct confirmed by authors (e-mail). tytsai88@gmail.com Biochem. J. (0264-6021) 2012 mimix curation shih-2012-5 Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Shih J.W., Wang W.T., Tsai T.Y., Kuo C.Y., Li H.K., Wu Lee Y.H. Origin of GST-PABP1 construct confirmed by authors (e-mail). tytsai88@gmail.com Biochem. J. (0264-6021) 2012 mimix curation pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV ddx3x_human ATP-dependent RNA helicase DDX3X DDX3X DBX DDX3 DEAD box protein 3, X-chromosomal Helicase-like protein 2 DEAD box, X isoform CAP-Rf protein protein human Homo sapiens Human MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV pabpc1-ddx3x 1311877 n/a 1311882 unspecified role unspecified role bait bait n/a 1311883 unspecified role unspecified role prey prey physical association physical association false false false 1A ddx3x-pabpc1-1 1311877 n/a 1311883 unspecified role unspecified role bait bait n/a 1311882 unspecified role unspecified role prey prey physical association physical association false false false 1B ddx3x-pabpc1-2 1311878 n/a 1311882 unspecified role unspecified role neutral component neutral component n/a 1311883 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1C ddx3x-eif4e-2 1311878 n/a 1311883 unspecified role unspecified role neutral component neutral component n/a 1311884 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1C ddx3x-eif4e-5 1311879 n/a 1311884 unspecified role unspecified role neutral component neutral component n/a 1311883 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2A, S6 and S8 ddx3x-pabpc1-3 1311879 n/a 1311882 unspecified role unspecified role neutral component neutral component n/a 1311883 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2B, 4, S4, S5, S6, S8 ddx3x-eif4e-4 1311880 n/a 1311884 unspecified role unspecified role prey prey n/a 1311882 unspecified role unspecified role prey prey n/a 1311883 unspecified role unspecified role bait bait association association false false false 1D ddx3x-pabpc1-4 1311881 n/a 1311882 unspecified role unspecified role neutral component neutral component n/a 1311883 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S1