mpidb The Microbial Protein Interaction Database roux-2011-1 Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Roux C.M., DeMuth J.P., Dunman P.M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation roux-2011-2 Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Roux C.M., DeMuth J.P., Dunman P.M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation rnpa_bacsu Ribonuclease P protein component rnpA Protein C5 BSU41050 protein protein bacsu Bacillus subtilis MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTLDQPENDELRVGLSVSKKIGNAVMRNRIKRLIRQAFLEEKERLKEKDYIIIARKPASQLTYEETKKSLQHLFRKSSLYKKSSSK rnj2_bacsu Ribonuclease J2 rnjB ymfA BSU16780 protein protein bacsu Bacillus subtilis MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENIGGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQQVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQLNIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHSKIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHLVSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV 8EA92EE42D51F282 csha_bacsu DEAD-box ATP-dependent RNA helicase CshA cshA ydbR BSU04580 protein protein bacsu Bacillus subtilis MTITFQDFNLSSDLMKAINRMGFEEATPIQAQTIPLGLSNKDVIGQAQTGTGKTAAFGIPLVEKINPESPNIQAIVIAPTRELAIQVSEELYKIGQDKRAKVLPIYGGQDIGRQIRALKKNPNIIVGTPGRLLDHINRRTIRLNNVNTVVMDEADEMLNMGFIDDIESILSNVPSEHQTLLFSATMPAPIKRIAERFMTEPEHVKVKAKEMTVSNIQQFYLEVQERKKFDTLTRLLDIQSPELAIVFGRTKRRVDELAEALNLRGYAAEGIHGDLTQAKRMVALRKFKEGAIEVLVATDVAARGLDISGVTHVYNFDVPQDPESYVHRIGRTGRAGKTGMAMTFITPREKSMLRAIEQTTKRKMDRMKEPTLDEALEGQQQVTVERLRTTISENNLNFYMTAAAELLEDHDAVTVVAAAIKMATKEPDDTPVRLTDEAPMVSKRYKNQRSSKRRDGQGGGYRGGKGKSNNRSSYDKKRSNDRRSSGDRRQKKSY rnj1_bacsu Ribonuclease J1 rnjA ykqC BSU14530 protein protein bacsu Bacillus subtilis MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV 48091B448B8431C9 rny_staar Ribonuclease Y rny cvfA SAR1262 Conserved virulence factor A protein protein strain mrsa252 Staphylococcus aureus MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVNQAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQRVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDGRIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQNVLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKYGENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETLSESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTVVRETRAVEYAK csha_staar DEAD-box ATP-dependent RNA helicase CshA cshA SAR2168 protein protein strain mrsa252 Staphylococcus aureus MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILGQAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVTVFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGFIDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEFYTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQAKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRAGKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSKESESRLKRISTELLNEYNDVDLVAALLQELIEANDEVEVQLTFEKPLSRKGRNGKPSGSRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK eno_staar Enolase eno 2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase SAR0832 protein protein strain mrsa252 Staphylococcus aureus MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK rnpa_staar Ribonuclease P protein component rnpA Protein C5 SAR2799 protein protein strain mrsa252 Staphylococcus aureus MEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFRLGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSLEHVLKIAKVFNKKIK rnj2_staar Ribonuclease J 2 rnj2 SAR1251 protein protein strain mrsa252 Staphylococcus aureus MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMFPEDEMLGIDIVIPDISYVLENKEKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI rnj1_staar Ribonuclease J 1 rnj1 SAR1063 protein protein strain mrsa252 Staphylococcus aureus MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPDDNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALGLIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVVHTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKIFRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPPETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPIPGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGEYRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDIGNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKIKTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK pnp_staar Polyribonucleotide nucleotidyltransferase pnp pnpA SAR1250 Polynucleotide phosphorylase protein protein strain mrsa252 Staphylococcus aureus MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKYIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPTERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE pfka_staar ATP-dependent 6-phosphofructokinase pfkA Phosphohexokinase pfk SAR1777 protein protein strain mrsa252 Staphylococcus aureus MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQFINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI rnpa-rnjb 1338665 not-defined-791146480 1338667 unspecified role unspecified role bait bait not-defined-688056724 1338668 unspecified role unspecified role prey prey physical association physical association false false false figure 3 rnpa-csha 1338665 not-defined-742286613 1338669 unspecified role unspecified role prey prey not-defined-1314381269 1338667 unspecified role unspecified role bait bait physical association physical association false false false figure 3 rnpa-rnpa 1338665 not-defined-1445257881 1338667 unspecified role unspecified role bait bait not-defined-1691185760 1338667 unspecified role unspecified role prey prey physical association physical association false false false figure 3 rnja-rnjb 1338665 not-defined-1866999901 1338668 unspecified role unspecified role prey prey not-defined-94397441 1338670 unspecified role unspecified role bait bait physical association physical association false false false figure 3 rnja-rnja 1338665 not-defined-1036536788 1338670 unspecified role unspecified role bait bait not-defined-1171775235 1338670 unspecified role unspecified role prey prey physical association physical association false false false figure 3 rny-rny 1338665 not-defined-1793946920 1338671 unspecified role unspecified role prey prey not-defined-111395651 1338671 unspecified role unspecified role bait bait physical association physical association false false false figure 2 y2168-rny-1 1338665 not-defined-1377677597 1338671 unspecified role unspecified role prey prey not-defined-900936729 1338672 unspecified role unspecified role bait bait physical association physical association false false false figure 2 eno-rny-2 1338665 not-defined-352639688 1338671 unspecified role unspecified role prey prey not-defined-269278167 1338673 unspecified role unspecified role bait bait physical association physical association false false false figure 2 rnpa-rnpa-1 1338665 not-defined-1791564311 1338674 unspecified role unspecified role bait bait not-defined-1748161017 1338674 unspecified role unspecified role prey prey physical association physical association false false false figure 2 y2168-rnpa 1338665 not-defined-368540718 1338674 unspecified role unspecified role prey prey not-defined-48007188 1338672 unspecified role unspecified role bait bait physical association physical association false false false figure 2 rnj2-rnj1 1338665 not-defined-732650751 1338675 unspecified role unspecified role bait bait not-defined-1858404579 1338676 unspecified role unspecified role prey prey physical association physical association false false false figure 2 pnp-pnp-1 1338665 n/a 1338677 unspecified role unspecified role prey prey not-defined-1701834204 1338677 unspecified role unspecified role bait bait physical association physical association false false false figure 2 eno-eno-4 1338665 n/a 1338673 unspecified role unspecified role prey prey not-defined-269278167 1338673 unspecified role unspecified role bait bait physical association physical association false false false figure 2 rnpa-y2168 1338665 not-defined-48007188 1338672 unspecified role unspecified role prey prey not-defined-368540718 1338674 unspecified role unspecified role bait bait physical association physical association false false false figure 2 rnj1-rnj2 1338665 not-defined-393010223 1338676 unspecified role unspecified role bait bait not-defined-884910309 1338675 unspecified role unspecified role prey prey physical association physical association false false false figure 2 eno-rny 1338665 not-defined-1632409702 1338673 unspecified role unspecified role bait bait not-defined-1630710306 1338671 unspecified role unspecified role prey prey physical association physical association false false false figure 2 y2168-pfka 1338665 not-defined-2035831693 1338678 unspecified role unspecified role prey prey not-defined-1754955129 1338672 unspecified role unspecified role bait bait physical association physical association false false false figure 2 y2168-eno 1338665 not-defined-81989050 1338673 unspecified role unspecified role prey prey not-defined-823570391 1338672 unspecified role unspecified role bait bait physical association physical association false false false figure 2 pnp-pnp 1338665 not-defined-1293035605 1338677 unspecified role unspecified role prey prey not-defined-1598753574 1338677 unspecified role unspecified role bait bait physical association physical association false false false figure 2 pfka-pfka 1338665 not-defined-1357381960 1338678 unspecified role unspecified role bait bait not-defined-426453503 1338678 unspecified role unspecified role prey prey physical association physical association false false false figure 2 eno-pnp 1338665 not-defined-959580254 1338677 unspecified role unspecified role prey prey not-defined-1531760819 1338673 unspecified role unspecified role bait bait physical association physical association false false false figure 2 rnj2-rnj2-1 1338665 not-defined-1348017011 1338675 unspecified role unspecified role unspecified role unspecified role not-defined-666342695 1338675 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false figure 4 rnj1-rnj2-1 1338665 not-defined-521135752 1338675 unspecified role unspecified role suppressor suppressor gene suppressor not-defined-1368754135 1338676 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false figure 4 rnj2-2 1338666 n/a 1338675 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4 rnj1-rnj2-2 1338666 n/a 1338676 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 n/a 1338675 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4