mpidb The Microbial Protein Interaction Database plocinski-2011-1 Characterization of CrgA, a new partner of the Mycobacterium tuberculosis peptidoglycan polymerization complexes. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Plocinski P., Ziolkiewicz M., Kiran M., Vadrevu S.I., Nguyen H.B., Hugonnet J., Veckerle C., Arthur M., Dziadek J., Cross T.A., Madiraju M., Rajagopalan M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation plocinski-2011-2 Characterization of CrgA, a new partner of the Mycobacterium tuberculosis peptidoglycan polymerization complexes. ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Plocinski P., Ziolkiewicz M., Kiran M., Vadrevu S.I., Nguyen H.B., Hugonnet J., Veckerle C., Arthur M., Dziadek J., Cross T.A., Madiraju M., Rajagopalan M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation plocinski-2011-3 Characterization of CrgA, a new partner of the Mycobacterium tuberculosis peptidoglycan polymerization complexes. mycsm Mycobacterium smegmatis fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Plocinski P., Ziolkiewicz M., Kiran M., Vadrevu S.I., Nguyen H.B., Hugonnet J., Veckerle C., Arthur M., Dziadek J., Cross T.A., Madiraju M., Rajagopalan M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation pbpb_myctu Penicillin-binding protein PbpB pbpB Penicillin-binding protein 3 ftsI pbp3 Rv2163c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MSRAAPRRASQSQSTRPARGLRRPPGAQEVGQRKRPGKTQKARQAQEATKSRPATRSDVAPAGRSTRARRTRQVVDVGTRGASFVFRHRTGNAVILVLMLVAATQLFFLQVSHAAGLRAQAAGQLKVTDVQPAARGSIVDRNNDRLAFTIEARALTFQPKRIRRQLEEARKKTSAAPDPQQRLRDIAQEVAGKLNNKPDAAAVLKKLQSDETFVYLARAVDPAVASAICAKYPEVGAERQDLRQYPGGSLAANVVGGIDWDGHGLLGLEDSLDAVLAGTDGSVTYDRGSDGVVIPGSYRNRHKAVHGSTVVLTLDNDIQFYVQQQVQQAKNLSGAHNVSAVVLDAKTGEVLAMANDNTFDPSQDIGRQGDKQLGNPAVSSPFEPGSVNKIVAASAVIEHGLSSPDEVLQVPGSIQMGGVTVHDAWEHGVMPYTTTGVFGKSSNVGTLMLSQRVGPERYYDMLRKFGLGQRTGVGLPGESAGLVPPIDQWSGSTFANLPIGQGLSMTLLQMTGMYQAIANDGVRVPPRIIKATVAPDGSRTEEPRPDDIRVVSAQTAQTVRQMLRAVVQRDPMGYQQGTGPTAGVPGYQMAGKTGTAQQINPGCGCYFDDVYWITFAGIATADNPRYVIGIMLDNPARNSDGAPGHSAAPLFHNIAGWLMQRENVPLSPDPGPPLVLQAT crga_myctu Cell division protein CrgA crgA MTCY10H4.11c Rv0011c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MPKSKVRKKNDFTVSAVSRTPMKVKVGPSSVWFVSLFIGLMLIGLIWLMVFQLAAIGSQAPTALNWMAQLGPWNYAIAFAFMITGLLLTMRWH ftsq_myctu Cell division protein FtsQ ftsQ Rv2151c MTCY270.17 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTEHNEDPQIERVADDAADEEAVTEPLATESKDEPAEHPEFEGPRRRARRERAERRAAQARATAIEQARRAAKRRARGQIVSEQNPAKPAARGVVRGLKALLATVVLAVVGIGLGLALYFTPAMSAREIVIIGIGAVSREEVLDAARVRPATPLLQIDTQQVADRVATIRRVASARVQRQYPSALRITIVERVPVVVKDFSDGPHLFDRDGVDFATDPPPPALPYFDVDNPGPSDPTTKAALQVLTALHPEVASQVGRIAAPSVASITLTLADGRVVIWGTTDRCEEKAEKLAALLTQPGRTYDVSSPDLPTVK pbpa_myctu Peptidoglycan D,D-transpeptidase PbpA pbpA Penicillin-binding protein A MTCY10H4.16c Rv0016c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKGAVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATETEQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGRAVIEAALQGEP ftsz_myctu Cell division protein FtsZ ftsZ MTCY270.18 Rv2150c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTPPHNYLAVIKVVGIGGGGVNAVNRMIEQGLKGVEFIAINTDAQALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDEIEELLRGADMVFVTAGEGGGTGTGGAPVVASIARKLGALTVGVVTRPFSFEGKRRSNQAENGIAALRESCDTLIVIPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFADVKGIMSGAGTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDVSGPGRKPVMGETGGAHRIESAKAGKLTSTLFEPVDAVSVPLHTNGATLSIGGDDDDVDVPPFMRR pbpb-y011 1338445 not-defined-1180477467 1338448 unspecified role unspecified role bait bait not-defined-1317880367 1338449 unspecified role unspecified role prey prey physical association physical association false false false figure 5 ftsq-y011 1338445 not-defined-726901492 1338450 unspecified role unspecified role bait bait not-defined-839564522 1338449 unspecified role unspecified role prey prey physical association physical association false false false figure 5 pbpa-y011 1338445 not-defined-1168837611 1338449 unspecified role unspecified role prey prey not-defined-143916924 1338451 unspecified role unspecified role bait bait physical association physical association false false false figure 5 y011-ftsz 1338445 not-defined-41740483 1338449 unspecified role unspecified role bait bait not-defined-291418449 1338452 unspecified role unspecified role prey prey physical association physical association false false false figure 4A y011-y011 1338445 not-defined-1418701193 1338449 unspecified role unspecified role prey prey not-defined-1930466492 1338449 unspecified role unspecified role bait bait physical association physical association false false false figure 4A ftsz-y011-1 1338445 not-defined-291418449 1338452 unspecified role unspecified role bait bait not-defined-41740483 1338449 unspecified role unspecified role prey prey physical association physical association false false false figure 4A y011-ftsz-1 1338446 not-defined-1243624547 1338449 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1932313043 1338452 unspecified role unspecified role prey prey physical association physical association false false false Figure 4B ftsz-y011 1338446 not-defined-1920753942 1338452 unspecified role unspecified role bait bait p64170-- P64170-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-236463622 1338449 unspecified role unspecified role prey prey physical association physical association false false false figure 4B ftsz-y011-3 1338447 not-defined-41740483 1338449 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-291418449 1338452 unspecified role unspecified role neutral component neutral component region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false figure 3B