mpidb The Microbial Protein Interaction Database kommineni-2011-3 YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kommineni S., Garg S.K., Chan C.M., Zuber P. B. subtilis genomic DNA-activation domain J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation kommineni-2011-2 YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO). bacsu Bacillus subtilis pull down pull down western blot western blot Immuno blot Immuno blot Kommineni S., Garg S.K., Chan C.M., Zuber P. B. subtilis genomic DNA-activation domain J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation kommineni-2011-1 YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kommineni S., Garg S.K., Chan C.M., Zuber P. B. subtilis genomic DNA-activation domain J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation spxo_bacsu Anti-adapter protein SpxO spxO yirB yuzO BSU33029 protein protein bacsu Bacillus subtilis MRELDEMISRLRNRGIKVEKVKYPKQTLSEKKWVHQCKQPLKTNYRDFNGYSFT spxh_bacsu ClpXP adapter protein SpxH spxH yjbH BSU11550 protein protein bacsu Bacillus subtilis MTNYQHELYFAHCHGHPKKPLEIYMFVDPLCPECWSLEPVIKKLKIRYGRFFTLRIIASASLTALNKKRKKHLLAEAWEKIASRSGMSCDGNVWFEQDQPLSSPYMAALAFKAAELQGRKAGMQFLRNMQESLFVSKKNITDENVLLEIAENTSLDLEEFKKDLHSQSAVKALQCDMKIAAEMDVSVNPTLTFFNTQHEDEGLKVPGSYSYDVYEEILFEMLGDEPKPSETPPLECFIEYFRFVASKEIALVYDLSLEEVEKEMKKLAFAKKVAKVEAKHGMFWKSLSTYSDEYQSCEK yqah_bacsu Uncharacterized protein YqaH BSU26310 yqaH protein protein bacsu Bacillus subtilis MNTNHFLKADVPIAKRKIESAEELSIMLSEALRDGDYEEAISLAGSIKVLTEDISRLANKGRLYETALKMQQQGINLTVVSRCIG fliz_bacsu Flagellar biosynthetic protein FliZ cheA BSU16340 fliZ protein protein bacsu Bacillus subtilis MKKSQYFIVFICFFVLFSVHPIAAAAADSDNSTVNEWFQKKDEKTADQSEQKKEKTTKTADETEGAAAPSVSAFDFVKMIFALLFVIVLIYGLVKLMNKRNRLLKPFQYVENIGGTSVGQNRSIQLIKVGKSVLVVGVGETIQLLKEIEDEKEIEVILSQHEEAMSSKIEWQKFVKPLKSSEHQPQQKLPSFSKALKEQLEELKQNRSEGKKKGPRHHE ctsr_bacsu Transcriptional regulator CtsR yacG Class three stress gene repressor BSU00830 ctsR protein protein bacsu Bacillus subtilis MGHNISDIIEQYLKRVLDQNGKEILEIKRSEIADKFQCVPSQINYVINTRFTSERGYIVESKRGGGGYIRIIKIKMNNEVVLINNIISQINTHLSQAASDDIILRLLEDKVISEREAKMMVSVMDRSVLHIDLPERDELRARMMKAMLTSLKLK mana3_bacsu Putative mannose-6-phosphate isomerase YvyI yvyI pmi Phosphohexomutase Phosphomannose isomerase BSU35790 protein protein bacsu Bacillus subtilis MTQSPIFLTPVFKEKIWGGTALRDRFGYSIPSESTGECWAISAHPKGPSTVANGPYKGKTLIELWEEHREVFGGVEGDRFPLLTKLLDVKEDTSIKVHPDDYYAGENEEGELGKTECWYIIDCKENAEIIYGHTARSKTELVTMINSGDWEGLLRRIKIKPGDFYYVPSGTLHALCKGALVLETQQNSDATYRVYDYDRLDSNGSPRELHFAKAVNAATVPHVDGYIDESTESRKGITIKTFVQGEYFSVYKWDINGEAEMAQDESFLICSVIEGSGLLKYEDKTCPLKKGDHFILPAQMPDFTIKGTCTLIVSHI yosl_bacsu SPbeta prophage-derived uncharacterized protein YosL yosL BSU20080 protein protein bacsu Bacillus subtilis MGAARRIDPTQPYVKKKNIINFTVATENTHIHLADGQSFPVLKGEIIATDQQGNQFVELEKNLDDYVPVKKSSLYESMAQGYMEMGDINREISEAFNYAENEAETVTTKLITGAYND gabr_bacsu HTH-type transcriptional regulatory protein GabR gabR ycnF BSU03890 protein protein bacsu Bacillus subtilis MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAGTQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLHFVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD bshc_bacsu Putative cysteine ligase BshC bshC yllA BSU15120 protein protein bacsu Bacillus subtilis MQLTELSIKNQNVFVQHYIDGKEEMSSFFDYSIHHKDMWRERLEDLSSRFFAREELAAYLTSYHNKFGSSAMQSAIEKLKDPSSAAVVGGQQAGLLTGPLYTIHKIISIIVLAKQQEKELQVPVIPIFWVAGEDHDLDEINFVHTSEENGPVKKKLPQSYWKKSSAASTSLDQEKCAAWIDDVFAAFEETDHTNTLLDNVKRCLRESVTFTDFFELLIADLFQEEGLVLLNSGDPGIKKLETAMFQKILRENDELARAVSDQQAFMRQAGYKPIIESGKEQANLFYEYEDERFLIEKDNGRFVIKELDLGWTRDELHTHMEEHPERFSNNVVTRPLMQEFLIPTLAFIAGPGEINYWGELKQAFAVMGFKMPPVMPRLNITILERHIEKKLAERNISLQDAIERGTENQRETYFERQIPEEFTAVMDQAKSQIEAIHKTVRQEALKVDQSLEPLLLKNAAFIQDQLQFLERTVMKRIEEKEGYVLKDYERIQNSIKPLLAPQERIWNIMYYLNRYGPKFFTTFKNLPFSFQNQHQVVKL yuxj_bacsu Uncharacterized MFS-type transporter YuxJ yuxJ yugC ORF1 BSU31480 protein protein bacsu Bacillus subtilis MWLANFFVSASTTMIVPFLSLYIETLSSFSNGFVQRWSGYVFGITFLMAFLVSPFWGRFGDKRGYKKILMATGTGIALSIFFMGFVTSVYQLFFLRMAMGLVTGFIPTSLAMISAQTPKSSAGKTLGTLQMGQVSGSLFGPLLGGMLADRFGFTYTFFITSFVIFSSVLLVLFGVKEKHLAEKTAKRTSYSRKEVLSYIFHHPALWVMMLLTMLIQTGNFSIQPLLALYVNELHGPVNLAFFSGMAFSATGLGSLLLARKWGDLGDRYGHRRILIGLLLAASFFFIPQALASSLSVLLVFRFLFGMAMGGLLPCITAAIRVQAPGSIQGEVLGYNVSFRFLGNVLGPLLGGIISSHFTISATFYVTAFLFFAGACMLWIMQKLRKDSYAKAS spx_bacsu Global transcriptional regulator Spx spx Redox-responsive transcription factor Spx Suppressor of clpP and clpX spxA yjbD BSU11500 protein protein bacsu Bacillus subtilis MVTLYTSPSCTSCRKARAWLEEHEIPFVERNIFSEPLSIDEIKQILRMTEDGTDEIISTRSKVFQKLNVNVESMPLQDLYRLINEHPGLLRRPIIIDEKRLQVGYNEDEIRRFLPRKVRSFQLREAQRLAN yjbh-yuzo-1 1338274 not-defined-115313550 1338277 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-yqah 1338274 n/a 1338279 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-fliz 1338274 n/a 1338280 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-ctsr 1338274 n/a 1338281 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-yvyi 1338274 n/a 1338282 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-yosl 1338274 n/a 1338283 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-gabr 1338274 n/a 1338284 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-ylla 1338274 n/a 1338285 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yjbh-yuxj 1338274 n/a 1338286 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait physical association physical association false false false Supp. Table 1 yuzo-yjbh 1338275 not-defined-1964526716 1338278 unspecified role unspecified role prey prey not-defined-2072790157 1338277 unspecified role unspecified role bait bait o32302-- O32302-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 3C spxa-yjbh-2 1338275 not-defined-1321419501 1338287 unspecified role unspecified role bait bait o31602-- O31602-- strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1662763011 1338278 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4 YjbH did not coelute with Spx-Strep when YirB-His was included in the reaction mixture yjbh-yuzo 1338276 not-defined-115313550 1338277 unspecified role unspecified role prey prey not-defined-1646198639 1338278 unspecified role unspecified role bait bait region O31606-1-24 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 1