UniProt UniProt http://www.uniprot.org bhatia-2011-2 Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bhatia N., Yang B., Xiao T.Z., Peters N., Hoffmann M.F., Longley B.J. Cancer - Interactions investigated in the context of cancer Ranges for binding regions taken from the corresponding UniProt features. Cells have been treated for 24h with small molecules compounds (with different concentrations , respectively) nbhatia@dermatology.wisc.edu Arch. Biochem. Biophys. (0003-9861) 2011 Only protein-protein interactions imex curation bhatia-2011-1 Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1. human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bhatia N., Yang B., Xiao T.Z., Peters N., Hoffmann M.F., Longley B.J. Cancer - Interactions investigated in the context of cancer Cells have been treated for 24h with small molecules compounds (with different concentrations , respectively) nbhatia@dermatology.wisc.edu Arch. Biochem. Biophys. (0003-9861) 2011 Only protein-protein interactions imex curation tif1b_human Transcription intermediary factor 1-beta TRIM28 KAP1 RNF96 TIF1B Tripartite motif-containing protein 28 Nuclear corepressor KAP-1 KRAB-associated protein 1 KRAB-interacting protein 1 RING finger protein 96 E3 SUMO-protein ligase TRIM28 RING-type E3 ubiquitin transferase TIF1-beta protein protein human Homo sapiens Human MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP magc2_human Melanoma-associated antigen C2 MAGEC2 HCA587 MAGEE1 Cancer/testis antigen 10 Hepatocellular carcinoma-associated antigen 587 MAGE-C2 antigen MAGE-E1 antigen protein protein human Homo sapiens Human MPPVPGVPFRNVDNDSPTSVELEDWVDAQHPTDEEEEEASSASSTLYLVFSPSSFSTSSSLILGGPEEEEVPSGVIPNLTESIPSSPPQGPPQGPSQSPLSSCCSSFSWSSFSEESSSQKGEDTGTCQGLPDSESSFTYTLDEKVAELVEFLLLKYEAEEPVTEAEMLMIVIKYKDYFPVILKRAREFMELLFGLALIEVGPDHFCVFANTVGLTDEGSDDEGMPENSLLIIILSVIFIKGNCASEEVIWEVLNAVGVYAGREHFVYGEPRELLTKVWVQGHYLEYREVPHSSPPYYEFLWGPRAHSESIKKKVLEFLAKLNNTVPSSFPSWYKDALKDVEERVQATIDTADDATVMASESLSVMSSNVSFSE maga3_human Melanoma-associated antigen 3 MAGEA3 MAGE3 Antigen MZ2-D Cancer/testis antigen 1.3 MAGE-3 antigen protein protein human Homo sapiens Human MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHMVKISGGPHISYPPLHEWVLREGEE 3F5EB13D1C9946A1 magec2-trim28-9 1294831 n/a 1294833 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1294834 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1294840 1294838 physical association physical association false false false 3B HTS11125 (CHEBI:64070); SBP022270 (CHEBI:64127) magea3-trim28-1 1294831 n/a 1294833 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1294835 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1294845 1294843 physical association physical association false false false 3A HTS11125 (CHEBI:64070); GK02432 (CHEBI:64072); SBP022270 (CHEBI:64127); magec2-trim28-8 1294832 n/a 1294834 unspecified role unspecified role bait bait n/a 1294833 unspecified role unspecified role prey prey physical association physical association false false false 2A HTS11125 (CHEBI:64070); GK02432 (CHEBI:64072); JFD00211 (CHEBI:64125); RF00201 (CHEBI:64126); SBP022270 (CHEBI:64127); SEW00166 (CHEBI:64128); KM09320 HTS04391