mpidb The Microbial Protein Interaction Database hale-2011-1 Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation hale-2011-2 Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation hale-2011-3 Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation hale-2011-4 Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers. ecolx Escherichia coli electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Hale C.A., Shiomi D., Liu B., Bernhardt T.G., Margolin W., Niki H., de Boer P.A. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation zapc_ecoli Cell division protein ZapC b0946 JW5125 zapC ycbW FtsZ-associated protein C Z-ring-associated protein C protein protein ecoli Escherichia coli (strain K12) MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAFSPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHGEMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKIMNDRLKPQVNVDSFSLEQAV ftsz_ecoli Cell division protein FtsZ ftsZ sfiB sulB b0095 JW0093 protein protein ecoli Escherichia coli (strain K12) MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNTDAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAAGMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSLITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMSEMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFETVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQPVMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD zapc-ftsz 1338193 not-defined-325151589 1338197 unspecified role unspecified role bait bait P75862:p.Leu22Pro P75862-22-22 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-1700544063 1338198 unspecified role unspecified role prey prey physical association physical association false false false Table 4 ftsz-zapc-4 1338193 not-defined-949541771 1338198 unspecified role unspecified role unspecified role unspecified role not-defined-160936023 1338197 unspecified role unspecified role unspecified role unspecified role P75862:p.Leu22Pro P75862-22-22 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 6 ftsz-zapc-5 1338194 not-defined-492638725 1338197 unspecified role unspecified role neutral component neutral component P75862:p.Leu22Pro P75862-22-22 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-334279727 1338198 unspecified role unspecified role neutral component neutral component physical association physical association false false false figure 5 ftsz-ftsz-7 1338195 n/a 1338198 unspecified role unspecified role neutral component neutral component n/a 1338198 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Fig. 6 Addition of ZapC enhanced light scattering by FtsZ polymers ftsz-ftsz-8 1338196 n/a 1338198 unspecified role unspecified role neutral component neutral component n/a 1338198 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Fig. 7 ZapC promotes associations between FtsZ filaments.