mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ turner-1990-2 Paxillin: a new vinculin-binding protein present in focal adhesions. in vitro In vitro solid phase assay solid phase assay autoradiography autoradiography Turner C.E., Glenney J.R., Burridge K. The source of paxillin and vinculin used in the experiments chicken gizzards, as described by the author. Contact email is not given by the authors in the paper. J. Cell Biol. (0021-9525) 1990 Only protein-protein interactions imex curation turner-1990-1 Paxillin: a new vinculin-binding protein present in focal adhesions. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography Turner C.E., Glenney J.R., Burridge K. The source of paxillin and vinculin used in the experiments chicken gizzards, as described by the author. Contact email is not given by the authors in the paper. J. Cell Biol. (0021-9525) 1990 Only protein-protein interactions imex curation paxi_chick Paxillin PXN protein protein chick Gallus gallus (Chicken) Chicken MDDLDALLADLESTTSHISKRPVFLTEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVIDPLDQWQPSVSRYGHQQPQSQSPIYSSSAKSSSASVPRDGLSSPSPRASEEEHVYSFPNKQKSAEPSPTMTSTSLGSNLSELDRLLLELNAVQHNPPSGFSADEVSRSPSLPNVTGPHYVIPESSSSAGGKAAPPTKEKPKRNGGRGIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQRVNASQQQTRISASSATRELDELMASLSDFKFMAQGKAGGSSSPPSTTPKPGSQLDTMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGVFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFINGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMGKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC vinc_chick Vinculin VCL VINC1 Metavinculin protein protein chick Gallus gallus (Chicken) Chicken MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQLADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMSEARKVAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKPVTVINEAAEAGVDIDEEDDADVEFSLPSDIEDDYEPELLLMPTNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ vcl-pxn 1295826 n/a 1295828 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard n/a 1295829 unspecified role unspecified role bait bait chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Figure 7a Vinculin-paxillin interaction was inhibited by the addition of increasing amounts of soluble vinculin. pxn-vcl 1295826 n/a 1295828 unspecified role unspecified role bait bait chick-gizzard Chicken gizzard gizzard gizzard n/a 1295829 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false 1295826 Figure 7b The vinculin-paxillin interaction was inhibited by the addition of increasing amounts of soluble vincluin. pxn-vcl 1295827 n/a 1295828 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard n/a 1295829 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false The sequence region is inferred from Unitprot. chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Figure 6B (Lane2'-8' and Lane9'); Figure 8B The radioiodinated paxillin probes both vinculin fractionated from embryo extracts (fig6B, lane2'-8') and also purified vinculin (figure 6B, lane 9' and fig 8B).