mpidb The Microbial Protein Interaction Database rowland-2010-1 Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B. ecolx Escherichia coli affinity techniques affinity technology affinity techniques tag fluorescence tag visualisation by fluorescence tag fluorescence Rowland SL, Wadsworth KD, Robson SA, Robichon C, Beckwith J, King GF J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation divib_bacsu Cell division protein DivIB dds Cell division and sporulation protein BSU15240 divIB Division initiation protein DivIB protein protein bacsu Bacillus subtilis MNPGQDREKIVNIEERIPKIKEQRKQKANRRLISFIMLFFIMVLIIVYLQTPISKVSTISVTGNENVSKKEIIDLSDINSGDTEFWSLDKQKTEKKIQQNKLVKKAEISKSLPNKINIAIEEYKAIAYLEKDDVYYEVLENGSVLPNEVTPDDAGPILVNWTNAKKRSQMAKQLDALSNSLKQSISEIYYTPVKMDENRIKLYMNDGYVVTASIKTFADRMKTYPSIISQLSSNKKGIIHLEVATYFEEFGKNDKAAKKEDEN pbp2b_bacsu Penicillin-binding protein 2B Penicillin-binding protein B pbpB Penicillin-sensitive peptidoglycan D,D-transpeptidase BSU15160 protein protein bacsu Bacillus subtilis MPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNVYLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISASTAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD divib-pbpb-3 1337848 not-defined-984363413 1337849 unspecified role unspecified role bait bait p16655-- P16655-- fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region P16655-123-263 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false not-defined-2121594230 1337850 unspecified role unspecified role prey prey q07868-- Q07868-- gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 2