mpidb The Microbial Protein Interaction Database younis-2010-2 SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation younis-2010-3 SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation younis-2010-1 SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal. eco57 Escherichia coli O157:H7 pull down pull down ms participant Identification by mass spectrometry ms participant Younis R., Bingle L.E., Rollauer S., Munera D., Busby S.J., Johnson S., Deane J.E., Lea S.M., Frankel G., Pallen M.J. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation q7db65_eco57 E5F08_27470 sepD ECs_4574 protein protein eco57 Escherichia coli O157:H7 MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHDFGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDNNHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV q7db79_eco57 E5F08_27385 sepL ECs_4557 protein protein eco57 Escherichia coli O157:H7 MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINLQNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKDIILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQRENALLMIGKVIDYKEEII q7db69_eco57 cesL E5F08_27450 ECs_4570 mpc protein protein eco57 Escherichia coli O157:H7 MNLLVKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDNNVFVTFALEYNENAFFSFFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQHVQIIERVRRMTS q7db51_eco57 E5F08_27540 ler ECs_4588 protein protein eco57 Escherichia coli O157:H7 MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQKQITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGMKKEDFLVKDTEEEIIPLKNI ch60_eco57 Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 groL mopA ECs5124 Z5748 protein protein eco57 Escherichia coli O157:H7 MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM odp1_eco57 Pyruvate dehydrogenase E1 component aceE Z0124 ECs0118 protein protein eco57 Escherichia coli O157:H7 MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEARKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTIKGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGELAKRGEIDKKVVADAIAKFNIDADKVNPRLA rl1_eco57 50S ribosomal protein L1 rplA Z5557 ECs4907 protein protein eco57 Escherichia coli O157:H7 MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN sepl-q7db65 1337815 n/a 1337818 unspecified role unspecified role prey prey ecolx Escherichia coli n/a 1337819 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false Fig. 4 sepl-q7db65-1 1337815 n/a 1337820 unspecified role unspecified role prey prey ecolx Escherichia coli n/a 1337818 unspecified role unspecified role prey prey ecolx Escherichia coli n/a 1337819 unspecified role unspecified role bait bait ecolx Escherichia coli association association false false false Fig. 4 q7db65-q7db69 1337816 n/a 1337820 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli n/a 1337819 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli n/a 1337818 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli physical association physical association false false false Fig. 5 q7db65-sepl-1 1337816 n/a 1337819 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli n/a 1337818 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false Fig. 5 q7db69-acee-2 1337817 n/a 1337821 unspecified role unspecified role prey prey n/a 1337822 unspecified role unspecified role prey prey n/a 1337823 unspecified role unspecified role prey prey n/a 1337824 unspecified role unspecified role prey prey n/a 1337820 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1337819 unspecified role unspecified role prey prey n/a 1337818 unspecified role unspecified role prey prey association association false false false Figure 3