IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ flowers-2010-1 The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu Development (0950-1991) 2010 Only protein-protein interactions imex curation flowers-2010-2 The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Flowers E.B., Poole R.J., Tursun B., Bashllari E., Pe'er I., Hobert O. or38@columbia.edu Development (0950-1991) 2010 Only protein-protein interactions imex curation unc37_caeel Transcription factor unc-37 unc-37 esg W02D3.9 Uncoordinated protein 37 protein protein caeel Caenorhabditis elegans MKASYLETLDRIKDEHAEMSKHVNQQRSDIEKVALEKENMNRSYMTYAEVSNTLRSDLRKAEEINKRLQEFIMQSLAPQLSQDNQANCLAALEAFKTASPRENGNGAPALPPGFPPGAAGMLGMMPNMPFGMSPAMSQLFNQFASPHVNGGDGAGGSSGGASEAKKAKLEDPDDGELEIDVTNDDHPSTASNGGAANKNGRDSTNSVASSGASTPSIASNSRARQQQQPLAGLQGLEQMNFLAGFNPNLLRQASAAGGFNFLNDPHAQARLAAAIGQIGSRPAYSFKIVDGGVPTPTSFPPDAQKGPGIPTGLKKKMELNHGEVVCAATISRDNSRVYTGGKGCVKIWDVKESDISGATVVNRPPIASLDCLKENYIRSCKLFEDGNTLLIGGEASTVALWDLTTETKTLDLETDSQACYALAMSPDEKLLFACLADGNILIYDIHNKVKVGTLPGHQDGASCLDLSKDGTKLWSGGLDNSVRCWDLAQRKEVAKHDFASQVFSLGCCPNDEWVAVGMENNYVEVLSTTGKEKYQLTQHESCVLSLKFAHSGKFFISTGKDNALNAWRTPYGASLFQLKENSSVLSCDISFDDSLIVTGSGEKKATLYAVEY q93616_caeel CELE_F27D4.2 F27D4.2 lsy-22 protein protein caeel Caenorhabditis elegans MSLADHIDAIKKEMTSLSSQFNSNKSELERSKQDLSSMQLQMQQQMNDSVQQDLQKAHDTSKKLDNLARTFLALITDPSQQSQLLAEFESISKNGTPQMPVMTPPMMNAVAAMQPHMMMRNPMLNAMGGASPRVSGKGGAPQNGGMNGMSMMQHMQMQAFQAQMMQAQMFQQMPMMMPGMVPGMPPGMAGLPGMMPPNNMAAAMQHFNAFQQMAAAASVAVSTPSRNPSTSGAASRTRTPLTTSATNSPRPTTEPTIKEEEPQTMEHDVAAAVAATTATATIKQEENSTVAVA g5ege2_caeel cog-1 CELE_R03C1.3 R03C1.3 protein protein caeel Caenorhabditis elegans MSLIMSEPPQLTTPDDKDVENRKHSSTYSISNLLLEKKESSPSGSSSEDDSASSNDDDQRSPSATSSFIFPPASSSSSSESATSPTQIMAQLMANGGAVDPGLQAYFFLLQSQLNSQSMMNRVTQENVSRALTSFNLLNNLPGALPSLSPMGRLQHSMQLSPNSLNMQKKQSRPTFTGHQIYQLERKFEQTKYLAGADRAQLAQELNMSESQVKVWFQNRRTKWRKKEAADNALVKRGASGDKSPCSLSPFLSAGI lsy_22-unc_37 1328285 n/a 1328287 unspecified role unspecified role prey prey n/a 1328288 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4A lsy_22-lsy_22 1328285 n/a 1328288 unspecified role unspecified role bait bait n/a 1328288 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A unc_37-cog_1 1328285 n/a 1328289 unspecified role unspecified role prey prey n/a 1328287 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4A unc_37-lsy_22-2 1328285 n/a 1328288 unspecified role unspecified role prey prey n/a 1328287 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4A lsy_22-unc_37-1 1328286 n/a 1328288 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region venus venus fluorescent protein tag venus undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1328287 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A