mpidb The Microbial Protein Interaction Database durand-2010-1 Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Durand E., Oomen C., Waksman G. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation durand-2010-2 Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. ecolx Escherichia coli bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Durand E., Oomen C., Waksman G. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation durand-2010-3 Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Durand E., Oomen C., Waksman G. J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation q0zkr5_ecolx traB DNQ45_22565 CDC27_28750 GFY34_23065 GFY34_26265 RCS44_P0031 EIZ93_17590 protein protein ecolx Escherichia coli MRAATATKPKKIDAYRKEPSVNKKYLPYSYHLNDYVISMENGDLMAFFKLDGRTHDCASDRELVTWHKDLNTLVKSFGTDHVELWTHEYHHEAKEYPDGEYDHFFPAYIDQYNRKLHGDSKQLINDLYLTVIYKQVGDKTQKFLAKFEKPTRDEIQQMQNEALEGLEDISEQILEAMKPYGIQQLGIYYRDKRGVEIPAPDKKEREELAEVDESDIFDEAIVIERNEPEPSQAHAYSKALEFLYFLANMEWAIVPVCRDRIREYIMDNRPVSSLWGDVVQIRTVDHNFYTTGIEFREYEEDTEPGQLNMLKEADFEYLLTQSFSCLSESSAKTFLTHQEKSLQETRDRAQSQLAQLGTALDMLTSREFVMGYHHGTVHVWDNDQNAVQRKARRVKVMLTGCGVVGGTLSLASEAAYYARLPGNQKWAPRPVPINSWNFLHFSPFHNFMRGKPDNNPWGPALTMFRTISGTPLYFNFHVTPLEELSYGKRPLGHALITGMSGEGKTTLLNFLLAQSMKYNPRLFVYDRDRGMEPFIRSVGGYYKVLQQGMPSGFAPLQIEPTKRNIALIKNLFRICVETTNNGPISATMATELAEGVDAVMGEGSLIPREARTVTILDGYVNEVVENGVSLKGLLREWTREGQYGWLFDNDKDSLDLSANDIFGFDLSEFIAAKEEVSSPARTPLMMYLLYRVRDSIDGKRRVIQCFDEFHAYLDDPVIEREVKRGIKTDRKKDAIYVFATQEPNDALSSRIGRTIMSQTVTKICLRDPEAIREDYAFLTDAEYDALMSITEHSRQFLVKQGQQSAIASFNLYPRNSDDIDADIKTMDNVLSVLSGEPQNAEIAHELVERLGNDPEVWLKEYWRLTA trab-1 1337175 not-defined-198554949 1337178 unspecified role unspecified role neutral component neutral component region Q0ZKR5-1-448 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 6.0 physical association physical association false false false Table 1 trab-trab-7 1337175 not-defined-390478465 1337178 unspecified role unspecified role neutral component neutral component region Q0ZKR5-442-866 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 6.0 physical association physical association false false false Table 1 trab-trab-2 1337176 not-defined-680393172 1337178 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Stoichiometry: 2.0 physical association physical association false false false Supp. Fig 2C trab-trab-4 1337176 not-defined-138827634 1337178 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Stoichiometry: 2.0 physical association physical association false false false Supplemental figure 2B trab-trab-5 1337177 not-defined-1826033094 1337178 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 physical association physical association false false false Supplemental figure 2C trab-trab-6 1337177 not-defined-1483193604 1337178 unspecified role unspecified role unspecified role unspecified role region Q0ZKR5-1-448 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 physical association physical association false false false Supplemental figure 2C