UniProt UniProt http://www.uniprot.org ashida-2010-1 A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Nat. Cell Biol. (1465-7392) 2010 mimix curation ashida-2010-2 A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Nat. Cell Biol. (1465-7392) 2010 mimix curation ashida-2010-3 A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Nat. Cell Biol. (1465-7392) 2010 mimix curation ashida-2010-4 A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Human HeLa cell cDNA Nat. Cell Biol. (1465-7392) 2010 mimix curation ashida-2010-5 A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ashida H., Kim M., Schmidt-Supprian M., Ma A., Ogawa M., Sasakawa C. In vitro ubiquitination assays (fig 2b, 4f and S5a) not included as origin/identity of some proteins cannot be established (E1, UbcH5b). sasakawa@ims.u-tokyo.ac.jp Human brain cDNA libraries. Nat. Cell Biol. (1465-7392) 2010 mimix curation nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE ipa9_shifl E3 ubiquitin-protein ligase ipaH9.8 ipaH9.8 Invasion plasmid antigen ipaH9.8 RING-type E3 ubiquitin transferase ipaH9.8 SFLP090 pWR501_0234 CP0226 protein protein shifl Shigella flexneri MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALPALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS tnip1_human TNFAIP3-interacting protein 1 TNIP1 KIAA0113 NAF1 Nef-associated factor 1 HIV-1 Nef-interacting protein Virion-associated nuclear shuttling protein Nip40-1 A20-binding inhibitor of NF-kappa-B activation 1 protein protein human Homo sapiens Human MEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKMLGELLEESQMEATRLRQKAEELVKDNELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPACPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSSHANAMALGPLPREDGNLMLHLQRLETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRKENEALKAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQQQASVTAGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ ikbkg-ipah9_8 1291155 n/a 1291160 unspecified role unspecified role bait bait n/a 1291161 unspecified role unspecified role prey prey physical association physical association false false false 2a and S3a and b tnip1-ipah9_8 1291155 n/a 1291162 unspecified role unspecified role bait bait n/a 1291161 unspecified role unspecified role prey prey physical association physical association false false false 4a, S3c and d ikbkg-ipah9_8-3 1291155 n/a 1291161 unspecified role unspecified role prey prey n/a 1291162 ancillary ancillary prey prey n/a 1291160 unspecified role unspecified role bait bait region modlys modified L-lysine residue ModLys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NEMO was ubiquitinated (cells treated with iE-DAP to induce Nod1-stimulated ubiquitination of GST-NEMO) association association false false false 5d ikbkg-ipah9_8-1 1291156 n/a 1291160 unspecified role unspecified role bait bait n/a 1291161 unspecified role unspecified role prey prey physical association physical association false false false 2a and S5b ipah9_8-ikbkg 1291156 n/a 1291161 unspecified role unspecified role bait bait n/a 1291160 unspecified role unspecified role prey prey physical association physical association false false false 2a tnip1-ipah9_8-1 1291156 n/a 1291161 unspecified role unspecified role prey prey n/a 1291162 unspecified role unspecified role bait bait physical association physical association false false false 4a ikbkg-ipah9_8-2 1291157 n/a 1291162 unspecified role unspecified role prey prey n/a 1291161 unspecified role unspecified role prey prey n/a 1291160 unspecified role unspecified role bait bait association association false false false 5a ikbkg-ipah9_8-4 1291157 n/a 1291161 unspecified role unspecified role prey prey n/a 1291160 unspecified role unspecified role bait bait physical association physical association false false false 5a ipah9_8-ikbkg-1 1291158 n/a 1291160 unspecified role unspecified role prey prey n/a 1291161 unspecified role unspecified role bait bait physical association physical association false false false no figure ipah9_8-tnip1 1291158 n/a 1291162 unspecified role unspecified role prey prey n/a 1291161 unspecified role unspecified role bait bait physical association physical association false false false no figure ipah9_8-ikbkg-2 1291159 n/a 1291161 unspecified role unspecified role bait bait n/a 1291160 unspecified role unspecified role prey prey physical association physical association false false false no figure ipah9_8-tnip1-1 1291159 n/a 1291161 unspecified role unspecified role bait bait n/a 1291162 unspecified role unspecified role prey prey physical association physical association false false false no figure