mpidb The Microbial Protein Interaction Database minamino-2009-1 Interaction of FliK with the bacterial flagellar hook is required for efficient export specificity switching. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Minamino T., Moriya N., Hirano T., Hughes K.T., Namba K. tohru@fbs.osaka-u.ac.jp Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation minamino-2009-2 Interaction of FliK with the bacterial flagellar hook is required for efficient export specificity switching. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Minamino T., Moriya N., Hirano T., Hughes K.T., Namba K. tohru@fbs.osaka-u.ac.jp Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation flgd_salty Basal-body rod modification protein FlgD fla FIV flaV STM1176 flgD protein protein salty Salmonella typhimurium MSIAVNMNDPTNTGVKTTTGSGSMTGSNAADLQSSFLTLLVAQLKNQDPTNPLQNNELTTQLAQISTVSGIEKLNTTLGAISGQIDNSQSLQATTLIGHGVMVPGTTILAGKGAEEGAVTSTTPFGVELQQPADKVTATITDKDGRVVRTLEIGELRAGVHTFTWDGKQTDGTTVPNGSYNIAITASNGGTQLVAQPLQFALVQGVTKGSNGNLLDLGTYGTTTLDEVRQII flik_salty Flagellar hook-length control protein flaE flaR STM1974 fliK protein protein salty Salmonella typhimurium MITLPQLITTDTDMTAGLTSGKTTGSAEDFLALLAGALGADGAQGKDARITLADLQAAGGKLSKELLTQHGEPGQAVKLADLLAQKANATDETLTDLTQAQHLLSTLTPSLKTSALAALSKTAQHDEKTPALSDEDLASLSALFAMLPGQPVATPVAGETPAENHIALPSLLRGDMPSAPQEETHTLSFSEHEKGKTEASLARASDDRATGPALTPLVVAAAATSAKVEVDSPPAPVTHGAAMPTLSSATAQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFTRQGQQSAQLRLHPEELGQVHISLKLDDNQAQLQMVSPHSHVRAALEAALPMLRTQLAESGIQLGQSSISSESFAGQQQSSSQQQSSRAQHTDAFGAEDDIALAAPASLQAAARGNGAVDIFA flge_salty Flagellar hook protein FlgE fla FV flaK STM1177 flgE protein protein salty Salmonella typhimurium MSFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFAGSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKLDENRNLVNMQGMQLTGYPATGTPPTIQQGANPAPITIPNTLMAAKSTTTASMQINLNSTDPVPSKTPFSVSDADSYNKKGTVTVYDSQGNAHDMNVYFVKTKDNEWAVYTHDSSDPAATAPTTASTTLKFNENGILESGGTVNITTGTINGATAATFSLSFLNSMQQNTGANNIVATNQNGYKPGDLVSYQINNDGTVVGNYSNEQEQVLGQIVLANFANNEGLASQGDNVWAATQASGVALLGTAGSGNFGKLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQILNTLVNLR flgd-flik 1336333 not-defined-1595331254 1336335 unspecified role unspecified role bait bait ecolx Escherichia coli not-defined-1869729653 1336336 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false Fig. 7A flge-flik 1336333 not-defined-1764123980 1336336 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-280888112 1336337 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false Fig. 7A flik-flge 1336334 not-defined-280888112 1336337 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-1764123980 1336336 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false Fig. 7C flik-flgd 1336334 not-defined-1595331254 1336335 unspecified role unspecified role prey prey ecolx Escherichia coli not-defined-1869729653 1336336 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false Fig. 7B