mpidb The Microbial Protein Interaction Database eswaramoorthy-2009-2 In vivo domain-based functional analysis of the major sporulation sensor kinase, KinA, in Bacillus subtilis. bacsu Bacillus subtilis bn-page blue native page bn-page anti tag western anti tag western blot anti tag western Eswaramoorthy P., Guo T., Fujita M. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation eswaramoorthy-2009-1 In vivo domain-based functional analysis of the major sporulation sensor kinase, KinA, in Bacillus subtilis. bacsu Bacillus subtilis anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Eswaramoorthy P., Guo T., Fujita M. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation eswaramoorthy-2009-3 In vivo domain-based functional analysis of the major sporulation sensor kinase, KinA, in Bacillus subtilis. bacsu Bacillus subtilis crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Eswaramoorthy P., Guo T., Fujita M. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation kina_bacsu Sporulation kinase A kinA Stage II sporulation protein F Stage II sporulation protein J gsiC scoD spoIIF spoIIJ BSU13990 protein protein bacsu Bacillus subtilis MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQFLVESYFYNEHHLMPCTFRFIKKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGHQSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISVKGKIVYVNSAMLSMLGAKSKDAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYKNQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFEAATYEDLIGKNIYDQLHPCDHEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCIPTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLAAGIAHEIRNPLTAIKGFLQLMKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHVTVKDEGEGIPEKVLNRIGEPFLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFPKK A8BB97FE844B32EE kina-kina-5 1336191 not-defined-1135428285 1336194 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 physical association physical association false false false Fig. 5A kina-1 1336191 not-defined-1135428285 1336194 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 4.0 physical association physical association false false false Fig. 5A kina-1 1336191 not-defined-1135428285 1336194 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 8.0 physical association physical association false false false Fig. 5A kina-kina 1336192 not-defined-1499091727 1336194 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1587709255 1336194 unspecified role unspecified role prey prey region P16497-377-606 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4 kina-kina-2 1336193 not-defined-113620503 1336194 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 4.0 physical association physical association false false false Fig 5B