mpidb The Microbial Protein Interaction Database lien-2009-1 Characterization of the Escherichia coli ClpY (HslU) substrate recognition site in the ClpYQ (HslUV) protease using the yeast two-hybrid system. ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Lien H.Y., Shy R.S., Peng S.S., Wu Y.L., Weng Y.T., Chen H.H., Su P.C., Ng W.F., Chen Y.C., Chang P.Y., Wu W.F. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation sula_ecoli Cell division inhibitor SulA sulA sfiA b0958 JW0941 protein protein ecoli Escherichia coli (strain K12) MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPMMTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSGLKIHSNLYH hslu_ecoli ATP-dependent protease ATPase subunit HslU hslU htpI b3931 JW3902 Unfoldase HslU Heat shock protein HslU protein protein ecoli Escherichia coli (strain K12) MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFRKKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL hslv_ecoli ATP-dependent protease subunit HslV hslV htpO b3932 yiiC Heat shock protein HslV JW3903 protein protein ecoli Escherichia coli (strain K12) MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVIAGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADETASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDICIYTNHFHTIEELSYKA hslu-sula 1335781 not-defined-1070879245 1335782 unspecified role unspecified role prey prey not-defined-775708636 1335783 unspecified role unspecified role bait bait P0A6H5:p.Val89Ile mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false p0a6h5-175-209 P0A6H5-175-209 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false sula-hslu 1335781 not-defined-1015462191 1335782 unspecified role unspecified role bait bait not-defined-1447544031 1335783 unspecified role unspecified role prey prey P0A6H5:p.Val89Ile P0A6H5-175-209 mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P0A6H5:p.Leu199Gln mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0A6H5:p.Ile186Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figs. 2B, 6, 7 hslu-hslv-10 1335781 n/a 1335783 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1335784 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3