mpidb The Microbial Protein Interaction Database ma-2009a-1 An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ma L.S., Lin J.S., Lai E.M. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation ma-2009a-2 An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Ma L.S., Lin J.S., Lai E.M. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation q7cun4_agrfc impK Atu4333 protein protein agrt5 Agrobacterium tumefaciens (strain C58 / ATCC 33970) Agrobacterium tumefaciens (strain C58) MSTDNPSSWQDLPTVVEITEESRRRNQNARNMAAILEDIVETDEQARPARGKVATLPVDKLLTGFHFGGEEVPTLVQSAAPLLNLAHLLRFSDAEPDPDQLRRACVEAITRYERDLASARISPERARAAHYVVCATVDDVVLSKPWGVRAGWARSGLVSTFHNDVTGGDRVFDILDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSSKGALELSRIRDSLYKTLIGQYGTFERELSPHWKGVDARHTPLRTMTALWTLLSLMALAFALGYLFFTLSLNNASDVTFERLAHLPPTETPSVLISNPPPHPPAQVEIPRVVEPPKTVAPRQPSRLEKLMAFLQPEVDKKLVTLADVNGRLRVRINNSGLFTTGSADVSPQFRDLIQRIGGALAAEKFRAVVIGYTDTVPIKTVEFPSNWHLSEARAKAVGDILSQFTGPDAILTEGRADSDPIADNKTEEGRQINRRTEIMVLTNPDEKLSDAGILSPPIENSSGQQPGEAQP a9cgf9_agrfc impL Atu4332 protein protein agrt5 Agrobacterium tumefaciens (strain C58 / ATCC 33970) Agrobacterium tumefaciens (strain C58) MNPLSYFYTIRSYVESYAALVGRRFLSLLWVIALCVVVWFYGYLIALGDFKPLGTVQARLIAIGIIVAVWLVYIIVTIYRGRKQDKELVDSIEREALANRQAEIGEIQTRLKEALALLRRVTKKRFGYIYDLPWYVIFGAPGSGKTTALTNSGLQFPLGDALGENAVKGIGGTRNCNWWFADEAILIDTAGRYTTQDDLDGSSKAGWEGFLGLLRRYRRSQPINGALVTLSIPDLLNRDPEEQRQELRSIRQRLSELDEYLHARVPVYIVLTKADLLHGFVEFFDGFNKTDRQQVWGTTFKLDESYSAENLPQRLTEEFELLQQRVDAMLIERLQQEQNAEIRGRIFRFPAELARLKDRLHEALAELCASSPLIEAPLLRGVYFASGTQPETEKSPAASRTRRSYFLSRLFKDVIFPEAALVTRDKRLSRRQLLVRRIAYAVSATAVAIVFTGWIFTYFANTQALAEADRKLGAYEQLVQGIPVREVADADFLRILPALDNLRDVNSGFARERVWNVSFGLDQEDKIAGRQRDAYQRALNALLLPRMIVQLQKQLKDEKDVTRTFNSLKLYGMLGGMGGLDRDFLTTQTHQMFASLYPGDGRAAAREALDQHAKALADGVLAPIELDARLIATARETIRDQAIGTRAYDILAGLPQVRELMEWTPATAFGPLGERAFERRSKAPMAEGIEGLFTADGYRRVVIPQVAHAARVALSEGWVRGSDDAIKGATVEQVAQAALQIYFDRFEKIWADTLSDLRVKPSQSLGDAVETTRALANERNIVVEAARSIAEATDLRPGANPAALASAAEGDATAAVLAATVNAADPYARLRDMLATKGAATTGEQPNGDKTGGSPSEQLLAHFKLLNEQLARSATTSDEVAKVFDVDSQLTKANQDLLQQARELPAPLDVWVAGVAADVGSLAVKSARSRIAELWTADSASLCSSIVTGRYPFDRASSRDVAIADFTRLFAPTGVFQSFFKQRMEPFVDKTTTPWSWKGTFGAAGIPSSAVAQFENADKISRAFFPNGSETPTVSINVKPVSLTNASSAVMLEIEGERVVYYHGPIQAKSITWPSRENTASLSRIAFQPGGWQQAKTENGDWSPFRLFDGANIENQSGELLRVRFENGVQAAEFDIQFGSVLNPFKLDAIASFACPAQF impk-impl 1335770 not-defined-182161701 1335772 unspecified role unspecified role bait bait not-defined-1280583051 1335773 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5A impk-impl-1 1335771 not-defined-1119228267 1335772 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1354513403 1335773 unspecified role unspecified role prey prey physical association physical association false false false Fig 5B