mpidb The Microbial Protein Interaction Database carrasco_ma-2009-1 The innate immunity role of cathepsin-D is linked to Trp-491 and Trp-492 residues of listeriolysin O. in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Carrasco-Marín E., Madrazo-Toca F., de los Toyos J.R., Cacho-Alonso E., Tobes R., Pareja E., Paradela A., Albar J.P., Chen W., Gomez-Lopez M.T., Alvarez-Dominguez C. deicme@humv.es Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation catd_human Cathepsin D CTSD CPSD protein protein human Homo sapiens Human MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL tacy_lismo Listeriolysin O LLO Thiol-activated cytolysin hlyA lisA lmo0202 hly protein protein lismo Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) MKKIMLVFITLILVSLPIAQQTEAKDASAFNKENSISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEVNYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVYAKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNKVDNPIE ctsd-hly-1 1335755 not-defined-1244059891 1335756 enzyme enzyme neutral component neutral component not-defined-2141096756 1335757 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cleavage cleavage reaction cleavage false false false Fig. 1