mpidb The Microbial Protein Interaction Database krajcikova-2009-1 Searching for protein-protein interactions within the Bacillus subtilis spore coat. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Krajcikova D., Lukacova M., Mullerova D., Cutting S.M., Barak I. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation krajcikova-2009-2 Searching for protein-protein interactions within the Bacillus subtilis spore coat. ecoli Escherichia coli (strain K12) pull down pull down western blot western blot Immuno blot Immuno blot Krajcikova D., Lukacova M., Mullerova D., Cutting S.M., Barak I. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation krajcikova-2009-3 Searching for protein-protein interactions within the Bacillus subtilis spore coat. ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Krajcikova D., Lukacova M., Mullerova D., Cutting S.M., Barak I. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation cotz_bacsu Spore coat protein Z cotZ BSU11740 protein protein bacsu Bacillus subtilis MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDTVPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILDFTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHH coty_bacsu Spore coat protein Y cotY BSU11750 protein protein bacsu Bacillus subtilis MSCGKTHGRHENCVCDAVEKILAEQEAVEEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVEKLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPELVDRTSPHKDKKHHHNG cote_bacsu Spore coat protein E cotE BSU17030 protein protein bacsu Bacillus subtilis MSEYREIITKAVVAKGRKFTQCTNTISPEKKPSSILGGWIINHKYDAEKIGKTVEIEGYYDINVWYSYADNTKTEVVTERVKYVDVIKLRYRDNNYLDDEHEVIAKVLQQPNCLEVTISPNGNKIVVQAEREFLAEVVGETKVVVEVNPDWEEDDEEDWEDELDEELEDINPEFLVGDPEE cotw_bacsu Spore coat protein W cotW BSU11770 protein protein bacsu Bacillus subtilis MSDNDKFKEELAKLPEVDPMTKMLVQNIFSKHGVTKDKMKKVSDEEKEMLLNLVKDLQAKSQALIENQKKKKEEAAAQEQKNTKPLSRREQLIEQIRQRRKNDNN cotv_bacsu Spore coat protein V cotV BSU11780 protein protein bacsu Bacillus subtilis MSFEEKVESLHPAIFEQLSSEFEQQIEVIDCENITIDTSHITAALSIQAFVTTMIIVATQLVIADEDLADAVASEILILDSSQIKKRTIIKIINSRNIKITLSADEIITFVQILLQVLNSILSELDVL coty-cotz-2 1335641 not-defined-1237659858 1335644 unspecified role unspecified role prey prey not-defined-317126296 1335645 unspecified role unspecified role bait bait physical association physical association false false false Table 2 coty-cotz 1335641 not-defined-1675786016 1335644 unspecified role unspecified role prey prey not-defined-792443057 1335645 unspecified role unspecified role bait bait physical association physical association false false false Table 2 cote-cote 1335641 not-defined-1741932703 1335646 unspecified role unspecified role prey prey not-defined-1825096997 1335646 unspecified role unspecified role bait bait physical association physical association false false false Table 2 cotw-cotv 1335641 not-defined-1216596458 1335647 unspecified role unspecified role bait bait not-defined-1011898008 1335648 unspecified role unspecified role prey prey physical association physical association false false false Table 2 coty-coty-2 1335641 not-defined-541353379 1335645 unspecified role unspecified role prey prey not-defined-780171888 1335645 unspecified role unspecified role bait bait physical association physical association false false false Table 2 cotv-cotw-1 1335641 not-defined-1011898008 1335648 unspecified role unspecified role bait bait not-defined-1216596458 1335647 unspecified role unspecified role prey prey physical association physical association false false false Table 2 cotz-coty 1335642 not-defined-2114605460 1335645 unspecified role unspecified role prey prey not-defined-589431374 1335644 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2 cotv-cotw 1335642 not-defined-2040841545 1335648 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1492719712 1335647 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2 cote-cote-2 1335643 not-defined-1741932703 1335646 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1825096997 1335646 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3