mpidb The Microbial Protein Interaction Database kawai-2009-3 Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. ecolx Escherichia coli adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Kawai Y., Daniel R.A., Errington J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation kawai-2009-1 Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. bacsu Bacillus subtilis pull down pull down ms participant Identification by mass spectrometry ms participant Kawai Y., Daniel R.A., Errington J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation kawai-2009-2 Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. bacsu Bacillus subtilis pull down pull down western blot western blot Immuno blot Immuno blot Kawai Y., Daniel R.A., Errington J. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation pbpi_bacsu Penicillin-binding protein 4B Penicillin-binding protein I yrrR BSU27310 pbpI protein protein bacsu Bacillus subtilis MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDGRGSFLDRNGRALTGQSEPAVVLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVILQQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASHTIGSTNQDPALLRKKYPDKESLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIKTTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPIYPGSVFKTVIAAAAIENNMVKPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSLAEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQLYNEKSGVIWGDEKDKSVKKAIAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGETIDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAGYFPADKPKYALVVLHMDTPGDKALTNSVFYDIVKKVHEIEINQK mreb_bacsu Cell shape-determining protein MreB mreB Actin-like MreB protein Rod shape-determining protein MreB BSU28030 protein protein bacsu Bacillus subtilis MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQTDTKSIVAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPYVMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIGGGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSAEAPEESDNMEIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELAADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKGKTR F554AAA82AD8853F pbph_bacsu Penicillin-binding protein H ykuA BSU13980 pbpH protein protein bacsu Bacillus subtilis MTEIGREPKKKSKGNRAIRMNLFFLAVFVLFTALIFKLGVVQIVEGEQHEEDAEKSNAKTAYYPAPRGKMYDRNQKVAVDNQSVPEIVYVSTSSTKTEDKIKTAKRLASFIHIDTEFLKERDLRDYWIAAHPKKAAALLKESESNLKGDQAYKLQIERVPDQELKAIQQDDEEMETAAIYTRFSSGNAYEPQIVKAMNPNKSNSNGKNGALLDEKKNSSQRPKNDLTYDEISIVSEHLEELPGIDIVNDWTRKYPYDKTLYSVFGGVTTPDQGLLSDRKDFYLTRGYANNDRVGKSYLEYQYEEYLNSHKEKVEYVEDNKGNVVSQKTIDKGSRGYDLQLSFDMELQAKVEKIIEEEVRNSRARGNYMLDRAFAVMMDPNNGDILSMAGKKIDLKTNKIEDYAIGAFTTQYEMGSAVKGATVLAGYQDGIPHYKYYIDAPMLLGTNLIKKSYTNMGTINELTALQKSSNVYMFNVAMHIAGVTYKPHGSLPADQNDLLKMRNYYSQFGLGVKTGIDLPQESAGMQTTPKTVGGLILDLAIGQYDTYTPLQMAQYISVIANGGYRVQPRIVTSIHKPGKKDQLGKAIEHRKPKVLNKINNSESDLKQVQTGMKLVTSSGTAKNTFTEDVSGKTGTAETFYYGTNRNWWGKKTYNLTFVGYYPSKKPKVAFSVVVPSVDDDDKINKIIAKRAIHAYAELEKKHSKK pbpd_bacsu Penicillin-binding protein 4 pbpD BSU31490 protein protein bacsu Bacillus subtilis MTMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYDRDGSLVSEIVSDHENRVLVPFNKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVKDKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQLEKKYTKNEILEAYLNTIYFNNGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLKDAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLPAALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRTNMQLKKLMSSVVKSGTGKKAYFNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTPTSVEYLHSISPQLSIWKGTLQAAY pbpa_bacsu Penicillin-binding protein 1A/1B ponA BSU22320 protein protein bacsu Bacillus subtilis MSDQFNSREARRKANSKSSPSPKKGKKRKKGGLFKKTLFTLLILFVLGVVGGAVTFAVMVSDAPSLDESKLKTPYSSTIYDKNGKEIAEVGAEKRTYVSIDEIPDVVKEAFIATEDARFYEHHGIDPVRIGGALVANFKDGFGAEGGSTITQQVVKNSLLSHQKTLKRKVQEVWLSIQLERNYSKDEILEMYLNRIYFSPRAYGIGKAAEEFFGVTDLSKLTVEQAATLAGMPQSPTAYNPVKNPDKAEKRRNIVLSLMKKQGFISDSQYNKAKKVAVKDEGVVSQKEYEKASTNKYSAFVEEVMKEIDEKSDVDPSADGLKIYTTLDTKAQDKLDELMDGDTVGFTEGMQGGVTLLDTKNGEVRAIGAGRNQPVGGFNYATQTKAQPGSTIKPILDYGPVIENKKWSTYEQIDDSAYTYSNGKPIRDWDRKYLGPISMRYALAQSRNIPALKAFQAVGKDTAVDFANGLGLGLTKDNVTEAYSIGGFGGNDGVSPLTMAGAYSAFGNNGTYNEPHFVKSIEFNDGTKLDLTPKSKSAMSDYTAFMITDMLKTAVKTGTGQLAQVPGVEVAGKTGTTNFDDNEVKRYNIASGGARDSWFVGYTPQYTAAVWTGMGENEAGKKSLSAEEQKVAKRIFAQLIADVDDGSGSFEKPDSVVEATVEKGSNPAKLAGPNTPSDKKLTEYFVKGTAPSTVSKTYEKEEKEETAKLSGLNVKYDKDNQSLTLSWNYDGDATFAVKQSVDGGSYSEIQNSSAKEAVISGVQPGSVYKFEVTAVSDDGKSTASTSYEVPKAEDDEDKKDQQQTDDEKQDDEKTQDDTQTDDSQKDDGQTDQDQTDDSTNDQDKKQDNTNTNPSDNNNQDQSNDNDNDNSNNQDTSDGDSNSGKNDSTGSDTNKNKTDTSNKTQTNSSSIEKTN pbp2b_bacsu Penicillin-binding protein 2B Penicillin-binding protein B pbpB Penicillin-sensitive peptidoglycan D,D-transpeptidase BSU15160 protein protein bacsu Bacillus subtilis MPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNVYLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISASTAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD pbpg_bacsu Penicillin-binding protein 2D ywhE BSU37510 pbpG protein protein bacsu Bacillus subtilis MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYASDGSKLGETNYGEKRYWVPLKDMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAFAKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQNYSKDEILEGYLNTIYYGHGAYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQQMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVYTTVDKRMQRIAEETITETVNAGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAKRQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGETYSPSNYNGYYANKPITLLQALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAMLANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSVTGRTIANRLTRTYAGKSGTTSADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIWADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCAAKHYTYFVKGTEPANVCYGAEPAKQTKDRLPSKEKPASEKKWWDKWLGRHH pbpc_bacsu Penicillin-binding protein 3 PSPB20 Penicillin-binding protein C ycsM yzsA BSU04140 pbpC protein protein bacsu Bacillus subtilis MLKKCILLVFLCVGLIGLIGCSKTDSPEDRMEAFVKQWNDQQFDDMYQSLTKDVKKEISKKDFVNRYKAIYEQAGVKNLKVTAGEVDKDDQDNKTMKHIPYKVSMNTNAGKVSFKNTAVLKLEKTDDEESWNIDWDPSFIFKQLADDKTVQIMSIEPKRGQIYDKNGKGLAVNTDVPEIGIVPGELGDKKEKVIKELAKKLDLTEDDIKKKLDQGWVKDDSFVPLKKVKPDQEKLVSEATSLQGVTRTNVSSRYYPYGEKTAHLTGYVRAITAEELKKKKEGTYSDTSNIGIAGLENVYEDKLRGTTGWKIYVPQTGEVIAEKKAKDGEDLHLTIDIKTQMKLYDELKDDSGAAVALQPKTGETLALVSAPSYDPNGFIFGWSDKEWKKLNKDKNNPFSAKFNKTYAPGSTIKPIAAAIGIKNGTLKADEKKTIKGKEWQKDSSWGGYSVTRVSERLQQVDLENALITSDNIYFAQNALDMGADTFTKGLKTFGFSEDVPYEFPIQKSSIANDKLDSDILLADTGYGQGQMQMSPLHLATAYTPFVDNGDLVKPTLIKKDSQTADVWHKQVVTKEGAADITKGLKGVVEDERGSAYQPVVKGITVAGKTGTAELKTSKDDKDGTENGWFVGYDYENKDLLVAMMIQNVQDRGGSHYVVEKAKKQFQSN pbpf_bacsu Penicillin-binding protein 1F pbpF ponA Penicillin-binding protein F BSU10110 protein protein bacsu Bacillus subtilis MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENREPVSINEIPKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKEAVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNAEGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSRYEGEVTMSDAITYSKNAPAVWTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPFFISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTPKITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIELKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS eftu_bacsu Elongation factor Tu P-40 BSU01130 tuf tufA protein protein bacsu Bacillus subtilis MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSKNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVVKGSALKALEGDAEWEAKIFELMDAVDEYIPTPERDTEKPFMMPVEDVFSITGRGTVATGRVERGQVKVGDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAKPGTITPHSKFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGIIHLPEGVEMVMPGDNTEMNVELISTIAIEEGTRFSIREGGRTVGSGVVSTITE BC096F47AAE6F72A dnac_bacsu Replicative DNA helicase dnaC BSU40440 protein protein bacsu Bacillus subtilis MTDLLNDRLPPQNIEAEQAVLGAIFLQPSALTLASEVLIPDDFYRMSHQKIYNAMLVLGDRGEPVDLVTVTSELANTDLLEEVGGISYLTDIANSVPTAANIEYYAKIVEEKSILRRLIRTATTIAQDGYTREDEVEDLLSEAEKTIMEVAQRKNTSAFQNIKDVLVQTYDNIEQLHNRKGDITGIPTGFTELDRMTAGFQRNDLIIVAARPSVGKTAFALNIAQNVATKTDESVAIFSLEMGAEQLVMRMLCAEGNINAQNLRTGNLTEEDWGKLTMAMGSLSNSGIYIDDTPGIRVSEIRAKCRRLKQESGLGMILIDYLQLIQGSGRSKDNRQQEVSEISRELKSIARELQVPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYDKETENKNIIEIIIAKQRNGPVGTVSLAFVKEYNKFVNLERRFDDAGVPPGA dnai_bacsu Primosomal protein DnaI dnaI ytxA BSU28980 protein protein bacsu Bacillus subtilis MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEYIEQSKNCSYCSEDENCNNLLEGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSMYIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGKGKGLYLYGKFGVGKTFMLAAIANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWVRDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAPIRLDGENRRHP mrec_bacsu Cell shape-determining protein MreC mreC Cell shape protein MreC Rod shape-determining protein MreC BSU28020 protein protein bacsu Bacillus subtilis MPNKRLMLLLLCIIILVAMIGFSLKGGRNTTWPEKVIGDTTGVFQNIFHTPAEFFAGIFENINDLKNTYKENERLREKLDGQTQYEAKLQELEEENKSLRDELGHVKSIKDYKPILATVIARSPDNWAKQVTINKGTQQNVAKDMAVTNEKGALIGKIKSSGLNNFTSAVQLLSDTDRNNRVATKISGKKGSKGYGLIEGYDKEKKRLKMTIIERKDKQDVKKGDLIETSGTGGVFPEGLTIGEVTDIESDSYGLTKVAYVKPAADLTDLNNVIVVNRDVPTVDTEEEGS pbpi-mreb 1335364 not-defined-1525386125 1335367 unspecified role unspecified role bait bait not-defined-950119020 1335368 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7 pbph-mreb 1335364 not-defined-571496482 1335368 unspecified role unspecified role prey prey not-defined-585335408 1335369 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7 pbpd-mreb 1335364 not-defined-1888467291 1335370 unspecified role unspecified role bait bait not-defined-1953697046 1335368 unspecified role unspecified role prey prey physical association physical association false false false mreb-mreb 1335364 not-defined-950119020 1335368 unspecified role unspecified role prey prey n/a 1335368 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6 pona-mreb 1335364 not-defined-2065682779 1335368 unspecified role unspecified role prey prey not-defined-1350435098 1335371 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6 pbpb-mreb 1335364 not-defined-1909406511 1335372 unspecified role unspecified role bait bait not-defined-2140548132 1335368 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7 pbpg-mreb 1335364 not-defined-1763104845 1335368 unspecified role unspecified role prey prey not-defined-148704217 1335373 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7 pbpc-mreb 1335364 not-defined-1277402207 1335374 unspecified role unspecified role bait bait not-defined-971134951 1335368 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7 pbpf-mreb 1335364 n/a 1335375 unspecified role unspecified role bait bait not-defined-2140548132 1335368 unspecified role unspecified role prey prey physical association physical association false false false mreb-tuf 1335365 n/a 1335376 unspecified role unspecified role prey prey not-defined-391008458 1335368 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1A dnac-dnai-2 1335365 n/a 1335376 unspecified role unspecified role prey prey n/a 1335377 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1335378 unspecified role unspecified role prey prey association association false false false Fig. 5A mrec-mreb 1335366 not-defined-1576504756 1335379 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-385740848 1335368 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5B