DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fernandes_alnemri-2009-3 AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S. Origin of ASC coinstructs according pmid 11967258. E_Alnemri@lac.jci.tju.edu Nature (0028-0836) 2009 imex curation Only protein-protein interactions fernandes_alnemri-2009-2 AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S. Purified protein has been incubated with or without 64-mer synthetic dsDNA and cross-linked with glutaraldehyde Origin of ASC coinstructs according pmid 11967258. E_Alnemri@lac.jci.tju.edu Nature (0028-0836) 2009 imex curation Only protein-protein interactions fernandes_alnemri-2009-4 AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. mouse-macrophage Mus musculus macrophage macrophage Macrophage crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S. NALP3-/- macrophages were transfected with poly(dA:dT), ASC pyroptosomes were then isolated and cross-linked with DSS. Origin of ASC coinstructs according pmid 11967258. E_Alnemri@lac.jci.tju.edu Nature (0028-0836) 2009 imex curation Only protein-protein interactions fernandes_alnemri-2009-1 AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line Human acute monocytic leukemia established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S. Origin of ASC coinstructs according pmid 11967258. E_Alnemri@lac.jci.tju.edu Nature (0028-0836) 2009 imex curation Only protein-protein interactions asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS aim2_human Interferon-inducible protein AIM2 AIM2 Absent in melanoma 2 protein protein human Homo sapiens Human MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKT aim2_mouse Interferon-inducible protein AIM2 Aim2 Gm1313 Ifi210 Interferon-inducible protein 210 Interferon-inducible protein p210 protein protein mouse Mus musculus Mouse MESEYREMLLLTGLDHITEEELKRFKYFALTEFQIARSTLDVADRTELADHLIQSAGAASAVTKAINIFQKLNYMHIANALEEKKKEAERKLMTNTKKRGTQKVENRSQAENCSAASATRSDNDFKEQAATEVCPQAKPQKKQMVAEQEAIREDLQKDPLVVTVLKAINPFECETQEGRQEIFHATVATETDFFFVKVLNAQFKDKFIPKRTIKISNYLWHSNFMEVTSSSVVVDVESNHEVPNNVVKRARETPRISKLKIQPCGTIVNGLFKVQKITEEKDRVLYGIHDKTGTMEVLVLGNPSKTKCEEGDKIRLTFFEVSKNGVKIQLKSGPCSFFKVIKAAKPKTDMKSVE pycard-aim2-3 1312131 n/a 1312135 unspecified role unspecified role bait bait c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false dapin_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1312136 unspecified role unspecified role prey prey c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false dapin_region required to bind necessary binding region required to bind range range range range false ecoli Escherichia coli (strain K12) physical association physical association false false false 2a aim2-pycard-4 1312131 n/a 1312136 unspecified role unspecified role bait bait dapin_region required to bind necessary binding region required to bind range range range range false c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1312135 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false 2b aim2-aim2 1312132 n/a 1312136 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 4D and S8 aim2-aim2-1 1312132 n/a 1312136 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1312136 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 4D and S8 Homooligomeric form only detected in presence of dsDNA aim2-aim2-3 1312133 n/a 1312137 unspecified role unspecified role neutral component neutral component n/a 1312137 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5e The homodimeric form was prevalent, some oligomeric assemblies are seen as faint bands, but only in the presence of poly (dA:dT). synthetic DNA poly(dA-dT)poly(dA-dT) aim2-1 1312133 n/a 1312137 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false 5e and S12 The homodimeric form was prevalent, some oligomeric assemblies are seen as faint bands, but only in the presence of poly (dA:dT). synthetic DNA poly(dA-dT)poly(dA-dT) pycard-aim2-1 1312134 n/a 1312135 unspecified role unspecified role bait bait n/a 1312136 unspecified role unspecified role prey prey physical association physical association false false false 2c