mpidb The Microbial Protein Interaction Database marteyn-2009-1 The thioredoxin reductase-glutaredoxins-ferredoxin crossroad pathway for selenate tolerance in Synechocystis PCC6803. ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Marteyn B., Domain F., Legrain P., Chauvat F., Cassier-Chauvat C. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation marteyn-2009-2 The thioredoxin reductase-glutaredoxins-ferredoxin crossroad pathway for selenate tolerance in Synechocystis PCC6803. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Marteyn B., Domain F., Legrain P., Chauvat F., Cassier-Chauvat C. Mol. Microbiol. (0950-382X) 2009 Only protein-protein interactions imex curation q55980_syny3 Ferredoxin sll0662 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MVIADLNFPPDHRSGLEPELGGDWRNFDDRSGLEPELGGELRERGVYVDEVTCIGCKNCAHVAPNTFTIEQEHGRSRAFSQNGDDEAVIQEAIDTCPVDCIHWVPYDELKHLEEKRKHQQIRPLGYPQINPHL glrx2_syny3 Probable glutaredoxin ssr2061 ssr2061 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MAVSAKIEIYTWSTCPFCMRALALLKRKGVEFQEYCIDGDNEAREAMAARANGKRSLPQIFIDDQHIGGCDDIYALDGAGKLDPLLHS p74746_syny3 slr0600 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MKLSSKNLDARLDTVYDAIVLGGGMGGLSAAIYLARYGLKCLVVEKGRGRSFWMQDLRNYVGLDPDTPGRDIITHSTQQALHWGADLLRGYVEDVTDEGDTLAVKVKVGKKDSLYPIFRTKYVIAATGIIDNLPQLEDMQNVYDYAGYTLHVCMICDGFDMWDQKAVLIAGTEGQINAAFVLNWFTPYITVLTHGLCTVGDEMKAKLADHGYPLHEAAITKFLGEDHKMSGVELVDGTVVEATTGLINMGSVYHNHYLKGIEGLEWDGENLVTNDMAQTSHPRIFALGDLKKGLNQVSVAVADGTLAATQIWRNIRRASEPRKWIH glrx1_syny3 Probable glutaredoxin slr1562 slr1562 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MANLFNWLPLLSGRQADGIKAKVEIYTWQTCPFCIRAKLLLWWKGVKFIEYKIDGDDQARQAMAARAEGRRTVPQIFVNDQGIGGCDQLYGLDSRGQLDPLLATPPNPA ftrc_syny3 Ferredoxin-thioredoxin reductase, catalytic chain ftrC Ferredoxin-thioredoxin reductase subunit B sll0554 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MTSSDTQNNKTLAAMKNFAEQYAKRTDTYFCSDLSVTAVVIEGLARHKEELGSPLCPCRHYEDKEAEVKNTFWNCPCVPMRERKECHCMLFLTPDNDFAGDAQDIPMETLEEVKASMA q55980-glrx2 1334850 not-defined-410847307 1334852 unspecified role unspecified role bait bait Q55980:p.Cys96Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false Q55980:p.Trp103_Val104delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q55980:p.[Cys53Ser;Cys56Ser;Cys59Ser] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q55980:p.Cys53Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-1799634379 1334853 unspecified role unspecified role prey prey P73492:p.Cys18Ser mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P73492:p.Cys36Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P73492:p.Cys15Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P73492:p.Leu22Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 4 p74746-glrx1 1334850 not-defined-962419058 1334854 unspecified role unspecified role bait bait P74746:p.Cys153Ser P74746-153-153 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P74746:p.Cys197Ser P74746-197-197 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false not-defined-328409461 1334855 unspecified role unspecified role prey prey P74593:p.Cys86Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P74593:p.Cys34Ser P74593-34-34 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P74593:p.Cys31Ser P74593-31-31 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 glrx2-glrx1 1334850 not-defined-1272270824 1334855 electron acceptor electron acceptor prey prey P74593:p.Cys34Ser P74593-34-34 mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P74593:p.Cys31Ser P74593-31-31 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P74593:p.Cys86Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false not-defined-1635061678 1334853 electron acceptor electron acceptor bait bait P73492:p.Cys36Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P73492:p.Cys18Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P73492:p.Leu22Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false glrx1-p74746-2 1334850 not-defined-1812269114 1334855 unspecified role unspecified role bait bait not-defined-2085961292 1334854 unspecified role unspecified role prey prey physical association physical association false false false Table 4 q55980-ftrc 1334850 n/a 1334856 unspecified role unspecified role prey prey Q55389:p.Cys56Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false not-defined-410847307 1334852 unspecified role unspecified role bait bait physical association physical association false false false Table 4 glrx2-q55980 1334851 not-defined-1799634379 1334853 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-410847307 1334852 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q55980:p.Trp103_Val104delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false Fig. 5