mpidb The Microbial Protein Interaction Database douet-2009-1 Erwinia chrysanthemi iron metabolism: the unexpected implication of the inner membrane platform within the type II secretion system. dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Douet V., Expert D., Barras F., Py B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation douet-2009-2 Erwinia chrysanthemi iron metabolism: the unexpected implication of the inner membrane platform within the type II secretion system. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Douet V., Expert D., Barras F., Py B. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation e0sdc8_dicd3 togA Dda3937_04712 protein protein dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) MAEVIFNKLEKVYSNGFKAVHGIDLTIKDGEFMVIVGPSGCAKSTTLRMLAGLETISGGEVRIGEKIVNNLAPKDRGIAMVFQNYALYPHMTVRENLAFGLKLSKMPKDKIEAQVNEAAKILELEELMDRLPRQLSGGQAQRVAVGRAIVKKPDVFLFDEPLSNLDAKLRASMRIRISDLHKQLKKSGHPATSVYVTHDQTEAMTMGDRICVMKLGHIMQVDTPDNLYHFPKNMFVAGFIGAPEMNIKPGKLLEENGQITLQVGQYTMSLNQAKQDKVRGYVGKDICFGVRPEYVTVSEAPFDGGHFQGELVRAENMGHEFFMYIKVDGFELTSRMPSDEARVIINNRMNSKVYFKFDMDKCHIFDAKTEQNISL e0sm39_dicd3 General secretion pathway protein F outF Dda3937_02417 protein protein dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) MALFQYQALNAQGKKSQGMQEADSARHARQLLREKGLVPVKIEEQRGEVTTRSGFSLSLRRSHRISASDLALLTRQLATLVAAALPLEEALDAVAKQSEKPKLSALMAAVRAKVVEGHSLAEAMGNFPGSFERLYCAMVAAGEASGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLTLVAISVVSILLSAVVPKVVEQFIHMKQALPLSTRLLMGVSDAVRAYGPWVVLLLVLAVMGFRVLLRQEKHRLVFHRRLLFLPLIGRIARGLNTARYARTLSILNSSAVPLLQAMRISGDVLTNDYARFRLGQATDAVREGVSLHKALEQTSLFPPMMRHMIASGERSGELDGMLTRAADNQDREFSAQMTLALGLFEPLLVVSMAGIVLFIVLAILQPILQLNTLMSM e0sif0_dicd3 acsD Dda3937_02854 protein protein dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) MNNRNHDVLSRMISEKAALHGLLNCLIKEFAIPEGYLRYEWPDEMKGIPPGAYFDGADWKGIPMMIGLPDQLQLFVMVDRRDTFGSQHYLSDVYLRQAQGDWQCPDFEPLVARLLAACEHIAGRKNPELYEQILQSQRLVSAIVSHNGRQRADAPLQHYLQSEQGLWFGHPSHPAPKARLWPAHLGQEQWAPEFQARAALHQFEVPVDGLHIGANGLTPQQVLDGFADQQPASPGHAIICMHPVQAQLFMQDARVQQLLRDNVIRDLGQSGRVASPTASIRTWFIDDHDYFIKGSLNVRITNCVRKNAWYELESTVLIDRLFRQLLDQHADTLGGLVAAAEPGVVSWSPAAAGELDSHWFREQTGGILRENFCRRTGAERSIMAGTLFARGVDLQPMIQTFLRTHYGEALDDNALLYWFDDYQTRLLRPVLSLFFNHGVVMEPHLQNSVLVHQQGRPQQVLLRDFEGVKLTDDLGIRYIDDDIHPRVRQSLLYSREQGWNRIMYCLFINHLSETILALSQGRPQLAPLMWRRVQQQLRAIQGELKQPSPELDALIAGHPVACKTNLKVRLAAEADRQASYVRLPSPWGHAVQHGSEVQHDERRHGDVRHEEARHGEVQHG e0sg67_dicd3 Protein translocase subunit SecA secA Dda3937_02235 protein protein dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) MLIKLLTKVFGSRNDRALRRMRKIVDVINRLEPDMETLSDDELKAKTQVFRDRIKKGETLESLLPEAFAVVREASKRVFGMRHFDVQLIGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGRGVHVVTVNDYLAQRDAENNRPLFEFLGLSVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRELYYALVDEVDSILIDEARTPLIISGPAEDSSELYMRVNKIIPHLIRQEKEDSDTFQGEGHFSVDEKARQVNLTERGLVKIEELLVKGGIMEEGESLYSPANIMLMHHVTAALRAHVLFTRDVDYIVKDDEVIIVDEHTGRTMQGRRWSDGLHQAVEAKENVKINNENQTLASITFQNYFRIYEKLAGMTGTADTEAFEFSSIYKLDTIVVPTNRPMIRKDLPDLVYMTEQEKIGAIIEDIKERTAKGQPVLVGTISIEKSEVVSHALTKAGIKHNVLNAKFHAMEADIVAQAGRSGAVTIATNMAGRGTDIVLGGSWQAEVAQQENPDEAQIAAIKAEWQRRHDDVLAAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSNMMRKLGMKEGEAIEHPWVTKAIANAQRKVENRNFDIRKQLLEYDDVANDQRRAIYTQRNELLDASDISETINSIREDVFKVTIDAHIPPQSLEEMWDVPGLEQRLKNDFDLELPIAEWLDKEPDLHEETLRERIYAQAVELYGRKEEVVGSDVMRNFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFAMFAAMLEGLKYEVISTLSKVQVRMPEEVEALEQQRREEAERLARQQQLSHQDEAGSPYADRKIGRNDPCPCGSGKKYKQCHGRLQK e0shd8_dicd3 Periplasmic serine endoprotease DegP-like degQ Dda3937_00697 protein protein dicd3 Dickeya dadantii (strain 3937) Erwinia chrysanthemi (strain 3937) MKKTSLLYSALALGIGLSLSSLPPANAALPAVVEGQALPSLAPMLEKVLPAVVSVHVEGTQVQRQRIPEEFKFFFGPNTPSEKQNSRPFEGLGSGVIINAEKGYVLTNNHVVNNADKIQVQLNDGREYDAKLIGRDEQTDIALLQLNDAKNLTEVKMADSDQLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGALVNLRGELIGINTAILAPSGGNVGIGFAIPSNMAQNLTQQLVEFGEVKRGLLGIKGSEMTSEIAKAFKVDAQRGAFVSEVIPKSAAAKAGIKAGDVLVSLDGKPISSFAELRAKIGTTAPGKTVRIGLLRDGKQQEVAVVLDNSANVTTNADTLSPALQGATLNNGQLKDGSKGVTIDNVAKDSAAAKVGLQKGDIIVGVNRERVENITQLRKILEAKPSVLALNIVRGDESIYLLLR gspf_dicch Type II secretion system protein F outF General secretion pathway protein F Pectic enzymes secretion protein OutF protein protein erwch Erwinia chrysanthemi Pectobacterium chrysanthemi Erwinia chrysanthemi MALFQYQALNAQGKKSQGMQEADSARHARQLLREKGLVPVKIEEQRGEAAPRSGFSLSFGRSHRIASDLALLTRQLATLVAALPLEEALDAVAKQSEKPKLSALMAAVRAKVVEGHSLAEAMGNFPGSFERLYCAMVAAGEASGHLDAVLNRLADYTEQRHEMRSRIQQAMIYPCVLTLVAISVVSILLSAVVPKVVEQFIHMKQALPLSTRLLMSASDAVRTYGPWVVLLLVLAIMGFRVLLRQEKHRLVFHRRLLFLPVVGRVARGLNTARYARTLSILNSSAVPLLQAMRISGDVLTNDYARFRLGQATDAVREGVTLHKALEQTALFPPMMRHMIASERRRARRHVNPRGDNQDREFSAQMTLVLGLFEPLLVVSMAGIVLFIVLAILQPILQLNTLMSM q93at8_dicch acsD protein protein erwch Erwinia chrysanthemi Pectobacterium chrysanthemi Erwinia chrysanthemi MNNRNHDVLSRMISEKAALHGLLNCLIKEFAIPEGYLRYEWPDEMKGIPPGAYFDGADWKGIPMMIGLPDQLQLFVMVDRRDTFGSQHYLSDVYLRQAQGDWQCPDFEPLVARLLAACEHIAGRKNPELYEQILQSQRLVSAIVSHNGRQRADAPLQHYLQSEQGLWFGHPSHPAPKARLWPAHLGQEQWAPEFQARAALHQFEVPVDGLHIGANGLTPQQVLDGFADQQPASPGHAIICMHPVQAQLFMQDARVQQLLRDNVIRDLGQSGRVASPTASIRTWFIDDHDYFIKGSLNVRITNCVRKNAWYELESTVLIDRLFRQLLDQHADTLGGLVAAAEPGVVSWSPAAAGELDSHWFREQTGGILRENFCRRTGAERSIMAGTLFARGVDLQPMIQTFLRTHYGEALDDNALLYWFDDYQTRLLRPVLSLFFNHGVVMEPHLQNSVLVHQQGRPQQVLLRDFEGVKLTDDLGIRYIDDDIHPRVRQSLLYSREQGWNRIMYCLFINHLSETILALSQGRPQLAPLMWRRVQQQLRAIQGELKQPSPELDALIAGHPVACKTNLKVRLAAEADRQASYVRLPSPWGHAVQHGSEVQHDERRHGDVRHEEARHGEVQHG outf-acsd-4 1334367 n/a 1334369 unspecified role unspecified role prey prey n/a 1334370 unspecified role unspecified role bait bait n/a 1334371 unspecified role unspecified role prey prey n/a 1334372 unspecified role unspecified role prey prey n/a 1334373 unspecified role unspecified role prey prey association association false false false fig. 1 A tail-specific protease was also identified by name but could not be mapped to a known sequence. outf-acsd-1 1334368 not-defined-891712117 1334374 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-357637960 1334375 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2