mpidb The Microbial Protein Interaction Database bao-2009-2 Four VirB6 paralogs and VirB9 are expressed and interact in Ehrlichia chaffeensis-containing vacuoles. human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line Human acute monocytic leukemia established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bao W., Kumagai Y., Niu H., Yamaguchi M., Miura K., Rikihisa Y. Ehrlichia-containing vacuoles were isolated from E. chaffeensis-infected THP-1 cells J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation bao-2009-1 Four VirB6 paralogs and VirB9 are expressed and interact in Ehrlichia chaffeensis-containing vacuoles. ehrch Ehrlichia chaffeensis far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Bao W., Kumagai Y., Niu H., Yamaguchi M., Miura K., Rikihisa Y. J. Bacteriol. (0021-9193) 2009 Only protein-protein interactions imex curation q2gi58_ehrcr virB9-1 ECH_0043 protein protein strain arkansas Ehrlichia chaffeensis MMNFYKIYTILLLVLVMMFNSSAYAINRVDNPVTVDSRIKTFVYSNNEIYNVVFNYGYHSYIEFSKGETIKMLAMGNTVSWKVKPVGNKLFIMPLEKNGRTNMLIETNKGRSYAFDLICRSANESSDDNNDQAGYSDLIDLTYIVRFYYPKTEEDFELNQVKLPDISVPSAVQKIKKHVVIKPNNTSNHYILETKSENQNILPVELFDDGKLTYFKFSNDNQIIPQIFIYNNSEKKVPCKMLLLQNYVIIKGVHKNLYLEYKSESVKITNKKL q2ggx1_ehrcr ECH_0497 protein protein strain arkansas Ehrlichia chaffeensis MFKIVLIISIFLLSSCGYHGCIKPQSVLFDEYSTTVEAGLEDGKKEHVAWVKSDLVLAGRQFLTIKVDTVNVNFCNNTKDVVNIFSSEGSTLDSSAKKFPVNIPNIMKGEKLNFRLSPALKFTVDKDICKNNNPKVYVQNTSKCMEDYLDTEYYIPVDNYDFQMNNLMYLKDRGSANKDKEKSWINFPLKILYHLDRKTYRDVSQMNISKLSSDRYQNNNDLFYLCVTDKRLNEYVTKIVEDMLNKTSSGNTAYSGDKKEQLISEKKESLLRKRDAVRKKYQKLYDAYTVNMLCGNLCNIPGYDKQKLDKNCFVVETNNMLNSCTIEKSGISGCNLNEAGQYSTVLPFLYLTHDSGITNFITVDDAVKKWQADNNSSSDIGLLTNHQYSISKDLDKNLYLELRVKDDLELIGKYTIEVTKDCSNHVKDSLYYTISKGPPTVKPGEQGTTPLNFINNEDTIVKLTGNDEVGELYFGVKDNGDGYENNTGYFNITVIAKKKIPKVISYMVERLKDSLERGLYGTSSTNSGGVNIIYNSIIENMHFIKIVNSLLVLYILMNALFYCAGFSKASIFELLTITLKISIIIYVIGPNSWDFFNNHLFKLFTEAPIQLISIMTGQDPDTSTSFEFLDIILYRFSLSESWLQILALLFAGPVGWVSVVLIFWGLVVLFLTIATAVITYLISIILIGLLLSIAPFFIICILFRRTKAIFDSWIKSLVQTAMQPVIIFASFALLTQVIDNIIYAMFNFESCDVCVLRPEFDIGITTIGFCLLEFLLPLGFSPVSVFTDNVRDSVNSDTVMFIGLPMPIVNILIFVIMANATKHFVGSSGEMCTSIFGSFANLSMVAEGAKESALGIIGMDQNSRMQRSRYQQMNEMNNQDSDGERRRDPLDLHSPSRSDDSTNRTSRGGVSIPENPPSSFGR q2ggx2_ehrcr ECH_0496 virB6 protein protein strain arkansas Ehrlichia chaffeensis MLKVSKSIIYVLMLFLINVNFICAKSTLAAEVESEKVAVYRTTIASNPLYAKVSEHAKYIGIAGFTAGFALIIGSIFFLNPATWYVALGMFLAGMAAIAFAAAKAAPFFACNWSFVRHPIMRFDTHDAKRNGNTEGSYKECEEPTKNYPDCTNRDFGSEEEYFRCLVTGDKDSTKPVSINNAKQNEPVCASQKFKKVDKYYWPKNRISSSNYIEVCYRNPIGSLNPITMIKRASEASSGNVTELLKKGEYPREGDYSTQEIKVAKYAMQGEVKCEVLQAGQEKVIHTITFQAVEKADKLCVYATKSIGIPLWPQVEIGCHMRPSGPPVPMCEKSTPVMSDDNKRIVSYDNSKCYSCYIDPACRGEVGPHIKSVFPMTSIIVSCIKGSLSNILTGQCGQNASKKVGFLKVAQDKLKKAVMAVLVLALILFAIKAALGGIQSPAELYMLIIKFALVVYFTQGSAMSHYYDQIVKLSIGLSDMVLQAGGSQTICDYSQSDYEDKYKYIAPWDRLDCRVLFYLGQQVNGGAATIVLGVLLSAGLFFATMLFNMKLMLCLIAIFAVIMLLLIMIWLVYIFLLSLIALTILILISPLMIPMVLFQATKGFFDGWIRQLMVYTLYPVILFAFLALLFTVFDNLYFEDLKFKRKEVNVLGQKRITFTLNDPRECDDPKYDNNLACMFGNIEFITKPAFFGFNAYAPDFKHGAEMLWMKLLMMVLIGFLFYHFLSSIAYIAAELAGDPRAGHVGSSFTPKSMLASIVGLAAKVQGSVGEQAKKAITDKLDDRKKSASDGGGKDELSGGSSEGAGDAAEGAGGGAGGSGAASGGGG q2ggw9_ehrcr ECH_0499 protein protein strain arkansas Ehrlichia chaffeensis MCNFIKKGFIKLLLLLVITACESNQHPVPRCVPADVFTETRTASVSAYFDSRSEDFIADNKSLGNIVKNDQVVRWKYTGYVTDGRPIVLKAEGMWTAWVNDVSAEKSVSFGDEDSEHYNQILSVERICGPYNKIEKTFVPDGHSECKVSCELITGVKDDLERGAYGPPCWFKNGYGAYLLFKRPDDPEPNETLDHMRYPVSPVMHIGYKPLELAGSDVFSTKNKPIMNSLCQKVQLEPGWKIYVKILDKYYYDNVGGYAITFLEGVKTEKQFSVFEWVRKQVRGELDKAGEQVFRHLVGNPIFKNFIFSVLTLFLVFGALAYIFGMVQSPFGDIIIRILKISLVILLISPSSWEFFYNHLLALFIHGIDQIIALINSHTGSYNPKEPFSFLDDMIVNKIFSPVIWKIKIRALIIADFSSIFAVCVIIIAVFIYIALCMYGCVIYLTAFVGITFLIGLFPLFLLGILFSQFKSLFDGWLTQCVSFAMQAILMFTLISLFGALIMNYYYRIFGFTTCYNEWLKVKICVLGKIGCIVDQSIFGWTPGQTYDPKVIGLTTDFNLNDRKSSGSARYKFTGGGAYIDVPPDRKHKDFRYVDYPFLDPDAKTDGNPFGVTVSQDSPFRQLSYYINALLSSNKKYVAARLVKDIEDELDVLEKNGTISLNSKNKVLQIIQQRRTKDNSEKKSELEKYKDEDFKSQIIDSIIADVIGSAAQERTPQEELNKQYDYWLILRVRAGYLIFWTEVGSLLLAALLIWQMRAFVQSVAVSLAGGNMMSQTIASMYEGGFMKIFSGIPVVGVVFQKIDQGIDGLKFAVGNYITAVARMPLNALKSIPYLGAAVKFTAGVTGALTSSYNEYDRSFSNNFKRLNYARAFIGAHLGFSPLDALKYLGGHVAGKMLGNRDGGLIHNAIEDRKAALDSLKAHILGPEKHRPSPYIPNKKKETEDDDARNPFSKETVGNKSGTVSPDEYKGGDKNHIIREGLRSEAGSALSDDYKDGDKSHIIREGLRSEAGSALLDGYKGGNGSDIIKGGLPTEGSTLLDRYGNVCVSEGNIEHALEVREQLKTMLDNAENDTALQTIQYDCDRLDNALHEYLGDKFDDVTQGYASSRDPMGHPQDLITEDLSKISASSDFRDLNAIDLGTQVQVGSDIGSDVLQQGSADGNRSEVEVSMDISKRDALLEQMELKGMEAASGTEAQLYDSAELSTPLDVTSSINNVSSEVSQQDLASNGEIEVSLDKDLELKNLDVVSGDIPDVEVTSSSDDVTQIYDSAQDRDSCLDIISSADDASSEVSQQDLASNGEIEVSLDKDLELKNLDVVSGDIPDVEVTSSSDDVTQVYDSTEDRDSCLDIVSSADDTSSEISQQDLEGNDGYEIDKDMGSSNESMLDEIPGGFADVIQDCSDASLSTPLGMSARDDTDATDTVEDPKENVLPQDVDLEDLDTTHSTNDIADFSGESSLFDATSESDSAAFVGEQSIDFSTEEGSDLPSEVVAQHDVDSQDSSVSVSDMAVADMEQGTEDSKYTDVDGLSDSVLDLGYLEGNVDNPDIEPEHDMSQQYSTETEELSDMLSDDVIEPEGVSETDTQVDSEESQDDSGTAGQVEDERVHDEVPYDTQDSTSGDKGDIAEHDDKDGVVEHEDTVDQSIDDTHEEEGKFTEGIVSESVTEPEGVSETDTQVDSEESQGDSSASDQVEDERVHDEVPYDTQDSTSGDKGDIVEHEDKDSVVEHEGTVDQSIDDTHEEEGKFTEGIVSESVTEPEGVSETDTQVDSEESQGDSSASGQVEDERVHDEVPYDTQDSTSGDKDDIVEHEDTVDQSIDDTHEEEGKFTEGIVSESVAEPEGVSETDTQVDSEESQGDSSASGQVEDERVHDEVPYDSQDDTQDSTSEDEGDVVEHGDKGDIVAHEDKDSVVEHEGTVDQSIDDTHEEEGKFTEGIVSESVTEPEEVSEADTQVDSEESQGDSSASDQVEDERVHDEVPYDIQDSTSGDKGDIAEHGDKDDIVEHEDTVDQSIDDTHEEEGKFTEGIVSESVTEPEEVSEADTQVDSEESQGDSSASDQVEDDKVHDEVPYDSQDDVEHGDKGDIVAHEDKDSVVEHEGTVDQSIDDTHEEEGKFTEGIVSESVAEPEGVSDVDTQVDSEESQGDSSASDQVEDDKVHDEVPYDSQDDTQDSTSEDEGDVVEHGDKGDIVEHEGTVDQSIDDTHEEEGKFTEGIVSESVAEPEEVSEADTQVDSEESQGDSSASDQVEDERVHDEVPYDAQDSTSGDKGDIAEHGDKDDIVEHDDKGGVVEHEDTVDQSIDDTHEEEGKFTEGIVSESVTEPEGVSETDTQVDSEESQGDSSASDQVEDERVHDEVPYDSQDDTQDSASEDEGDVVEHGDKGDIVEHEDKDSVVEHEGTVDQSIDDTYEEEGKFTEGIVSESVTEPEEVSEADTQVNNEESQGDSSASDQVEDERVHDEVPYDAQDSTSGDKGDIAEHGDKDDIVEHDDKDGVVEHDDKDGVVEHEDTVDQSIDDTHEEEGKFTEGIVSESVTEPEEVSEADTQVDETLQDLEHEDLKEGADDISDSETDENSSGNSDSVSDVEGLASLASIGEESTITQNVKTQSVQSSAILSMKDRDGVLDNKGIPSDQLDVKDTEKKKKESSKSKVENSDKKAKSTKATVTPKSLKKTFKVILSQCTQELSNKLSEAFDKLFIDPQSGKQKRRLSKQDIALMIEQLKAMIESLKDRKAQVTDPDEMKAIEESIKQAESTIQNLLNQE q2ggx0_ehrcr ECH_0498 protein protein strain arkansas Ehrlichia chaffeensis MKKFIILLLFFVTGCGVNCIEGSKGISGNSVVVEVSPTVKKVSKVLQPNWIDTELRVSKDQPPMRVAVTGALNMCPKDFLNPKNLIVPAVSCISDIKDISYKDESTYQNIKDVCSNASLRTKAANSEYLSGVAHTVSAFRLNKGDAFKVSLIPRKVKVTDCSSAALKELGVIYYSDDEIFEDSNCQQAIPAENICQNGVPKFYVKVNQECKESGGMAGAIPRLQGTEVSALVDNTDTPYGNKIFYSEDAKKNWINAPFVYDARIIKNQKVNVETLKKHCTIIKELNEKKIINHVDTISTSQFEYYQKQKAQGDITTDIIKELIDVINNFTTYDINCVCGTICNSKSKLVTGKEIATISNYGSDGNCIISGSYEYNKDATFEEISKAKLEDISKNANNSDPIIQGLTAFFGTRVTHKQEKDARNYYCFPQKVQGGSCYADISSQNIDSNSLKSNISYAYDSDESVTLRFAVIGEKQKYASYSGGYNIHVERICNFMYGKKLYMYIGDNPPTIFPGDPGTIELFVPDPENPESGTGVYMINKDGSEKKSGKIYLGIDVRGYENQFDYSSLPGIESANKYFVDFFIKRWNPNFSKVFVMIRDFLLRILYGVPKDTQVDNIAQALDVARTSKSQGAIQSIYANQTGAGSLWRALQALCSLYIVFTVLGYAIGVVKFTKYDLGVRIAKIAIVVGLISQGSWEFFSDHFFSIFIQGTSDLVAAFNGELDGDNSFRFLDTTLGILLTGEVWIRLLTLILTGPIGWLIFYIIFWAFFVFFMCVIEAIIAYLLTIVAVAFLATLAPIFITFIFFQLTKTLFDAWVKMLVNFSLQPIILFAALAFLNQVVLTVLYKVTSFTVCNQCYLGFDLPTSAVEEGAPYDICLVSVLLPVGYSPDLSITESIRESYVQGNGGLLGLPFDIASALILLLAAHAMRGFKGMSETIAHSISGSVAGLSASVYGATQALASIVGRDQETQNIIKEAMRNRKSTGKSDVDIVSRSNPNPESRGESVERSGGSDSDLPPKGHDGGLRTGISDKGDNVPQQPQSGDDIKEDSKTGRASEQLHNDVSPLSSSSVLDTTPFTLSYNEMQGADNLDSNPSVTRNDTSADGTSGMDSQDASSDAGSELHGDGDTGNVATTQDTGDNEGDGAQNGSRDNGENAGNASNVTEQDTSSGENDEVQGGSRDNSESADDASSITEQDSSSGESDEAQGGSRDNSESADDTSSVAEDTSSDESDEAQGGSRDNDENGDSTLSVAEQDTSSDESDEAQGGSRDNDENADDTSSVAEQDTSSDESDEAQGGSRDNDENADDTSSVAEQDTSSDESDEAQGGSRDNDENDDSASSVTEQDADSVSDGTEVSTSDGDNNLGNDQSTDNTLDGNENTSGNNDTDVQEEAGKASSVVESSDSDDSDGRKRGRKDINFLDGPQRKRLYMDEYSSVAEKIAGHESAIAVEEESYERGIVKKRGKKDQQE virb9_1-q2ggx1-2 1334305 not-defined-732507326 1334307 unspecified role unspecified role bait bait not-defined-8305916 1334308 unspecified role unspecified role prey prey n/a 1334309 unspecified role unspecified role prey prey association association false false false figure 6B q2ggw9-q2ggx0-1 1334305 n/a 1334308 unspecified role unspecified role prey prey n/a 1334309 unspecified role unspecified role prey prey not-defined-978883160 1334310 unspecified role unspecified role bait bait not-defined-1433817824 1334311 unspecified role unspecified role prey prey association association false false false figure 6B q2ggx0-q2ggw9-1 1334305 not-defined-929277020 1334308 unspecified role unspecified role prey prey undefined mutation Q2GGX1-1-545 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-909150773 1334311 unspecified role unspecified role bait bait n/a 1334310 unspecified role unspecified role prey prey association association false false false figure 6B q2ggx1-q2ggx1-1 1334305 not-defined-1400924318 1334307 unspecified role unspecified role prey prey not-defined-570312215 1334308 unspecified role unspecified role bait bait region Q2GGX1-1-545 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1334308 unspecified role unspecified role prey prey association association false false false figure 6B virb9_1-q2ggx1-1 1334306 not-defined-1722001487 1334308 unspecified role unspecified role prey prey region Q2GGX1-1-545 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-141502713 1334307 unspecified role unspecified role bait bait physical association physical association false false false figure 6B