mpidb The Microbial Protein Interaction Database teng-2008-1 Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens. ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Teng W.L., Bannam T.L., Parsons J.A., Rood J.I. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation teng-2008-2 Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens. ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Teng W.L., Bannam T.L., Parsons J.A., Rood J.I. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation teng-2008-3 Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Teng W.L., Bannam T.L., Parsons J.A., Rood J.I. J. Bacteriol. (0021-9193) 2008 Only protein-protein interactions imex curation q1plh3_clopf ERS852446_03029 pCW3_0037 tcpH pTet_030 G6Z03_15150 protein protein clostridium perfring Clostridium perfringens MENVKINIYSKFLYLKNKLTYKNILKAIVIIATIVIAYNCLTKIAYADGFLSYGNSDDPYKNIPEGWGMFISEKYRNNYALDIIDTHGWELIDKAITGLTNIVFSGIVMISWFGVNIFRFCFTNDIAAGFANQLNSVLGSLNNGIFNNFFMIVFMISLFSIVYNLYKKNFSAIGSQILAVAIIYATTAFLSNGAVGFLSKTTEFSKTIGTSAIVAINGKDNNEGSLEDYSKTVVGTLWGNFVQQPWVMLEFDGKVPLNDENMTTKNSINEEALNYSKDILSEPYGSDEREKALESINEKFNKELFSKNGINTKLVSVCVLFVITLIKMIILIAVGMIQIGFQLMSILLILLLPFIMLIAIVPFFGGTNIIKGIARKYLGTQLGIIVSSFVLALLVLVDTVTLTMFQAWGANLIIALLMQCTCWIMVIAFRKQLMNGLINLQNRIAPGTAGAFRFGAKPLDKGADIAKEYAVNPAMDRAYDIGDNLITKAQYAKEYTKGNLSLYKDRKVAQAIDFLGSKYDNLKNNKPEDIENANNPKENIDGVKDTKNKVNSNENINKKPVEKFDLNGVKATKPSNITSENIPNKNINGENKPNIDEDAVTKNDIKDPIENKDIAPKTIDENIKNNGNDNGTIQDKSPGSKENKTISEDEEITTKPEEIKTPEKTINKENLNELKNENTSADKKNNDSNSSNISVTEIPEDLVEEKSVEAINKAKNDVKANKEKINNNINSKNVESNPEVKENIEVASSPEVTEIKKKSTKKVENIKNNGQPKEKSADKKEIKEVSTKERVERVKSFMGVSNKDIKSKEKHLENKLEYIKKGNSKNFKKKGS q1plh8_clopf pCW3_0032 tcpC G6Z03_15175 protein protein clostridium perfring Clostridium perfringens MFRKNKENTKPKKEKKEKKSFFKKKDKLNMDLEDSVANLDKQVNGVKEKKLVSVMPFRIARAFIWVLIGILCFRGGVSVFKGNQSEALKKENEKILNNMEKQSSLETQAFSFAEEFAWDYFSRYPSDTQDFVRRITKYTTEQLANEMNNGTYSDVIYTSAFYFEKYSENQVNVSVKARVRVYTPKAGQEQTPQDQLQYDTNLVDYYLEVPIVFDKDMNMAVDALPVMTAPPEKAYFKNKEFSGTSENDADKTKKITDSVSQFFKAYYEQNQTQIDYFLVDGADIKGAGQKFSFNKIDRINIYKLSDKEFLAIVDLNVDSFGNAIKQGFNLTVVQEGDKFLVKTLEPRTSNIDLNNKSNN tcph-tcpc 1333535 not-defined-1111315230 1333538 unspecified role unspecified role bait bait region Q1PLH3-514-581 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false not-defined-831318538 1333539 unspecified role unspecified role prey prey physical association physical association false false false Figs.4, 7 tcph-tcph 1333535 not-defined-2000405099 1333538 unspecified role unspecified role bait bait region Q1PLH3-514-581 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false not-defined-1987688582 1333538 unspecified role unspecified role prey prey physical association physical association false false false Figs. 3, 4 tcpc-tcph 1333535 not-defined-622123320 1333538 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false not-defined-528750940 1333539 unspecified role unspecified role bait bait physical association physical association false false false tcph-tcph-1 1333536 not-defined-482805643 1333538 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-284777096 1333538 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5 tcph-tcpc-1 1333537 not-defined-831318538 1333539 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1111315230 1333538 unspecified role unspecified role bait bait region Q1PLH3-514-581 mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false Fig 8