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<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2013-02-01Z">
            <names>
                <shortLabel>mpidb</shortLabel>
                <fullName>The Microbial Protein Interaction Database</fullName>
            </names>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
            </xref>
        </source>
        <experimentList>
            <experimentDescription id="1333204">
                <names>
                    <shortLabel>obara-2008-1</shortLabel>
                    <fullName>Roles of charged residues in the C-terminal region of PomA, a stator component of the Na+-driven flagellar motor.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="18326582" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402824" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18190" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="663">
                        <names>
                            <shortLabel>vibal</shortLabel>
                            <fullName>Vibrio alginolyticus</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>pull down</shortLabel>
                        <fullName>pull down</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>western blot</shortLabel>
                        <fullName>western blot</fullName>
                        <alias typeAc="MI:0303" type="go synonym">Immuno blot</alias>
                        <alias typeAc="MI:1041" type="synonym">Immuno blot</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Obara M., Yakushi T., Kojima S., Homma M.</attribute>
                    <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2008</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959"/>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="1333205">
                <names>
                    <shortLabel>poma_vibal</shortLabel>
                    <fullName>Chemotaxis protein PomA</fullName>
                    <alias typeAc="MI:0301" type="gene name">pomA</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="O06873" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0006935" secondary="chemotaxis" version="SP_96"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0097588" secondary="archaeal or bacterial-type flagellum-dependent cell motility" version="SP_96"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002898"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" secondary="integral component of membrane" version="SP_96"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000540"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" secondary="plasma membrane" version="SP_96"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0006811" secondary="ion transport" version="SP_96"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_005381564.1"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6402837" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="663">
                    <names>
                        <shortLabel>vibal</shortLabel>
                        <fullName>Vibrio alginolyticus</fullName>
                    </names>
                </organism>
                <sequence>MDLATLLGLIGGFAFVIMAMVLGGSIGMFVDVTSILIVVGGSIFVVLMKFTMGQFFGATKIAGKAFMFKADEPEDLIAKIVEMADAARKGGFLALEEMEINNTFMQKGIDLLVDGHDADVVRAALKKDIALTDERHTQGTGVFRAFGDVAPAMGMIGTLVGLVAMLSNMDDPKAIGPAMAVALLTTLYGAILSNMVFFPIADKLSLRRDQETLNRRLIMDGVLAIQDGQNPRVIDSYLKNYLNEGKRALEIDE</sequence>
            </interactor>
            <interactor id="1333206">
                <names>
                    <shortLabel>o06874_vibal</shortLabel>
                    <alias typeAc="MI:0301" type="gene name">pomB</alias>
                    <alias typeAc="MI:0306" type="orf name">Vag1382_06400</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="O06874" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036737"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3WPW"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3WPX"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" secondary="integral component of membrane" version="TrEMBL_92"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" secondary="plasma membrane" version="TrEMBL_92"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006665"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR025713"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6402829" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="663">
                    <names>
                        <shortLabel>vibal</shortLabel>
                        <fullName>Vibrio alginolyticus</fullName>
                    </names>
                </organism>
                <sequence>MDDEDNKCDCPPPGLPLWMGTFADLMSLLMCFFVLLLSFSEMDVLKFKQIAGSMKFAFGVQNQLEVKDIPKGTSIIAQEFRPGRPEPTPIDVIMQQTMDITQQTLEFHEGESDRAGGTKRDEGKLTGGQSPATSTQNNESAEADMQQQQSKEMSQEMETLMESIKKALEREIEQGAIEVENLGQQIVIRMREKGAFPEGSAFLQPKFRPLVRQIAELVKDVPGIVRVSGHTDNRPLDSELYRSNWDLSSQRAVSVAQEMEKVRGFSHQRLRVRGMADTEPLLPNDSDDNRALNRRVEISIMQGEPLYSEEVPVIQ</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-18190-1" id="1333207">
                <names>
                    <shortLabel>poma-pomb</shortLabel>
                </names>
                <xref>
                    <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-744" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18190-1" refType="imex-primary" refTypeAc="MI:0662"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6402834" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>1333204</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="1333208">
                        <names>
                            <shortLabel>not-defined-1639128057</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402842" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1333205</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>bait</shortLabel>
                                    <fullName>bait</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="1333209">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6479456" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>his tag</shortLabel>
                                        <fullName>his tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">6-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">6-His-tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                            <feature id="1333210">
                                <names>
                                    <shortLabel>O06873:p.Lys203Glu</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6479458" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>mutation decreasing strength</shortLabel>
                                        <fullName>mutation decreasing interaction strength</fullName>
                                        <alias typeAc="MI:1041" type="synonym">mutation decreasing strength</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1133" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528801" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </startStatus>
                                        <begin position="203"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </endStatus>
                                        <end position="203"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                    </participant>
                    <participant id="1333211">
                        <names>
                            <shortLabel>not-defined-2009900482</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402841" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1333206</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>prey</shortLabel>
                                    <fullName>prey</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">Fig. 3</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
