<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2013-02-01Z">
            <names>
                <shortLabel>mpidb</shortLabel>
                <fullName>The Microbial Protein Interaction Database</fullName>
            </names>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
            </xref>
        </source>
        <experimentList>
            <experimentDescription id="1333026">
                <names>
                    <shortLabel>pilonieta-2008-1</shortLabel>
                    <fullName>The chaperone IpgC copurifies with the virulence regulator MxiE.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="18192384" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402399" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18181" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>pull down</shortLabel>
                        <fullName>pull down</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>weight by comassie</shortLabel>
                        <fullName>molecular weight estimation by coomasie staining</fullName>
                        <alias typeAc="MI:1041" type="synonym">weight by comassie</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0818" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218596" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Pilonieta M.C., Munson G.P.</attribute>
                    <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2008</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959"/>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="1333027">
                <names>
                    <shortLabel>ipgc_shifl</shortLabel>
                    <fullName>Chaperone protein IpgC</fullName>
                    <alias typeAc="MI:0301" type="gene name">ipgC</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">ippI</alias>
                    <alias typeAc="MI:0305" type="locus name">CP0129</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0A2U4" secondary="ipgc_shifl" version="SP_19" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6SCB" version="SP_108"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3GZ2" version="SP_108"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011716" version="SP_108"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P18008" secondary="ipgc_shifl" version="SP_19" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="ensemblbacteria" id="AAL72340" version="SP_108" refType="transcript"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1535618" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="YP_009062486.1"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_000055835.1"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3GYZ" version="SP_108"/>
                    <secondaryRef db="ensemblbacteria" id="AAL72340" version="SP_108" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3GZ1" version="SP_108"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005415" version="SP_108"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_085291.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" secondary="cytoplasm" version="SP_108"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011990" version="SP_108"/>
                    <secondaryRef db="dip" dbAc="MI:0465" id="DIP-45500N"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3KS2" version="SP_108"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_858262.1"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR016379" version="SP_108"/>
                    <secondaryRef db="ensemblbacteria" id="SF_p0129" version="SP_108" refType="gene"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" secondary="identical protein binding" version="SP_108"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="623">
                    <names>
                        <shortLabel>shifl</shortLabel>
                        <fullName>Shigella flexneri</fullName>
                    </names>
                </organism>
                <sequence>MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE</sequence>
            </interactor>
            <interactor id="1333028">
                <names>
                    <shortLabel>mxie_shifl</shortLabel>
                    <fullName>Transcriptional regulator MxiE</fullName>
                    <alias typeAc="MI:0301" type="gene name">mxiE</alias>
                    <alias typeAc="MI:0305" type="locus name">CP0144</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0A2S7" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6402407" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q04642" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q55292" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="ensemblbacteria" id="AAL72330" version="SP_106" refType="transcript"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_085307.1"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_000398259.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0043565" secondary="sequence-specific DNA binding" version="SP_106"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="YP_009062501.1"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_858277.1"/>
                    <secondaryRef db="ensemblbacteria" id="SF_p0144" version="SP_106" refType="gene"/>
                    <secondaryRef db="ensemblbacteria" id="AAL72330" version="SP_106" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0003700" secondary="DNA-binding transcription factor activity" version="SP_106"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018062"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018060"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="623">
                    <names>
                        <shortLabel>shifl</shortLabel>
                        <fullName>Shigella flexneri</fullName>
                    </names>
                </organism>
                <sequence>MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-18181-1" id="1333029">
                <names>
                    <shortLabel>mxie-ipgc</shortLabel>
                </names>
                <xref>
                    <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-772" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18181-1" refType="imex-primary" refTypeAc="MI:0662"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6402404" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>1333026</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="1333030">
                        <names>
                            <shortLabel>not-defined-1560219331</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402417" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1333027</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>prey</shortLabel>
                                    <fullName>prey</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="1333031">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6479029" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>his tag</shortLabel>
                                        <fullName>his tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">6-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">6-His-tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="562">
                                <names>
                                    <shortLabel>ecolx</shortLabel>
                                    <fullName>Escherichia coli</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                    </participant>
                    <participant id="1333032">
                        <names>
                            <shortLabel>not-defined-1416290352</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-6402416" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1333028</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>bait</shortLabel>
                                    <fullName>bait</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="1333033">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6479032" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>mbp tag</shortLabel>
                                        <fullName>maltose binding protein tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">maltose binding protein tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">maltose binding protein tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">mbp tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0578" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-705364" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="562">
                                <names>
                                    <shortLabel>ecolx</shortLabel>
                                    <fullName>Escherichia coli</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">Fig. 1</attribute>
                    <attribute name="comment" nameAc="MI:0612">Authors report dentification of IpgC confirmed by anti-tag western blot.</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
