UniProt UniProt http://www.uniprot.org taylor-2008-1 Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R. a.r.lehmann@sussex.ac.uk Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation taylor-2008-2 Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. human-mrc5 Human foetal lung fibroblast-like cell line mrc5 Human foetal lung fibroblast-like cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R. a.r.lehmann@sussex.ac.uk Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation taylor-2008-3 Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R. a.r.lehmann@sussex.ac.uk Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation taylor-2008-4 Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Taylor E.M., Copsey A.C., Hudson J.J., Vidot S., Lehmann A.R. a.r.lehmann@sussex.ac.uk Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation nse1_human Non-structural maintenance of chromosomes element 1 homolog NSMCE1 HSPC333 HSPC337 protein protein human Homo sapiens Human MQGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH smc6_human Structural maintenance of chromosomes protein 6 SMC6 SMC6L1 protein protein human Homo sapiens Human MAKRKEENFSSPKNAKRPRQEELEDFDKDGDEDECKGTTLTAAEVGIIESIHLKNFMCHSMLGPFKFGSNVNFVVGNNGSGKSAVLTALIVGLGGRAVATNRGSSLKGFVKDGQNSADISITLRNRGDDAFKASVYGNSILIQQHISIDGSRSYKLKSATGSVVSTRKEELIAILDHFNIQVDNPVSVLTQEMSKQFLQSKNEGDKYKFFMKATQLEQMKEDYSYIMETKERTKEQIHQGEERLTELKRQCVEKEERFQSIAGLSTMKTNLESLKHEMAWAVVNEIEKQLNAIRDNIKIGEDRAARLDRKMEEQQVRLNEAEQKYKDIQDKLEKISEETNARAPECMALKADVVAKKRAYNEAEVLYNRSLNEYKALKKDDEQLCKRIEELKKSTDQSLEPERLERQKKISWLKERVKAFQNQENSVNQEIEQFQQAIEKDKEEHGKIKREELDVKHALSYNQRQLKELKDSKTDRLKRFGPNVPALLEAIDDAYRQGHFTYKPVGPLGACIHLRDPELALAIESCLKGLLQAYCCHNHADERVLQALMKRFYLPGTSRPPIIVSEFRNEIYDVRHRAAYHPDFPTVLTALEIDNAVVANSLIDMRGIETVLLIKNNSVARAVMQSQKPPKNCREAFTADGDQVFAGRYYSSENTRPKFLSRDVDSEISDLENEVENKTAQILNLQQHLSALEKDIKHNEELLKRCQLHYKELKMKIRKNISEIRELENIEEHQSVDIATLEDEAQENKSKMKMVEEHMEQQKENMEHLKSLKIEAENKYDAIKFKINQLSELADPLKDELNLADSEVDNQKRGKRHYEEKQKEHLDTLNKKKRELDMKEKELEEKMSQARQICPERIEVEKSASILDKEINRLRQKIQAEHASHGDREEIMRQYQEARETYLDLDSKVRTLKKFIKLLGEIMEHRFKTYQQFRRCLTLRCKLYFDNLLSQRAYCGKMNFDHKNETLSISVQPGEGNKAAFNDMRALSGGERSFSTVCFILSLWSIAESPFRCLDEFDVYMDMVNRRIAMDLILKMADSQRFRQFILLTPQSMSSLPSSKLIRILRMSDPERGQTTLPFRPVTQEEDDDQR nse2_human E3 SUMO-protein ligase NSE2 NSMCE2 C8orf36 MMS21 Non-structural maintenance of chromosomes element 2 homolog MMS21 homolog E3 SUMO-protein transferase NSE2 protein protein human Homo sapiens Human MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE nse4a_human Non-structural maintenance of chromosomes element 4 homolog A NSMCE4A C10orf86 PP4762 protein protein human Homo sapiens Human MSGDSSGRGPEGRGRGRDPHRDRTRSRSRSRSPLSPRSRRGSARERREAPERPSLEDTEPSDSGDEMMDPASLEAEADQGLCRQIRHQYRALINSVQQNREDILNAGDKLTEVLEEANTLFNEVSRAREAVLDAHFLVLASDLGKEKAKQLRSDLSSFDMLRYVETLLTHMGVNPLEAEELIRDEDSPDFEFIVYDSWKITGRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRMEESHQEATEKEVERILGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARIRLDQDRLPVIEPVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEISEPVITPSQRQQKPSA nse3_human Non-structural maintenance of chromosomes element 3 homolog HCA4 Hepatocellular carcinoma-associated protein 4 MAGE-G1 antigen MAGEG1 Melanoma-associated antigen G1 NDNL2 Necdin-like protein 2 NSMCE3 protein protein human Homo sapiens Human MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS smc5_human Structural maintenance of chromosomes protein 5 SMC5 KIAA0594 SMC5L1 protein protein human Homo sapiens Human MATPSKKTSTPSPQPSKRALPRDPSSEVPSKRKNSAPQLPLLQSSGPFVEGSIVRISMENFLTYDICEVSPGPHLNMIVGANGTGKSSIVCAICLGLAGKPAFMGRADKVGFFVKRGCSRGMVEIELFRASGNLVITREIDVAKNQSFWFINKKSTTQKIVEEKVAALNIQVGNLCQFLPQDKVGEFAKLSKIELLEATEKSIGPPEMHKYHCELKNLREKEKQLETSCKEKTEYLQKMVQRNERYKQDVERFYERKRHLDLIEMLEAKRPWVEYENVRQEYEEVKLVRDRVKEEVRKLKEGQIPVTCRIEEMENERHNLEARIKEKATDIKEASQKCKQKQDVIERKDKHIEELQQALIVKQNEELDRQRRIGNTRKMIEDLQNELKTTENCENLQPQIDAITNDLRRIQDEKALCEGEIIDKRRERETLEKEKKSVDDHIVRFDNLMNQKEDKLRQRFRDTYDAVLWLRNNRDKFKQRVCEPIMLTINMKDNKNAKYIENHIPSNDLRAFVFESQEDMEVFLKEVRDNKKLRVNAVIAPKSSYADKAPSRSLNELKQYGFFSYLRELFDAPDPVMSYLCCQYHIHEVPVGTEKTRERIERVIQETRLKQIYTAEEKYVVKTSFYSNKVISSNTSLKVAQFLTVTVDLEQRRHLEEQLKEIHRKLQAVDSGLIALRETSKHLEHKDNELRQKKKELLERKTKKRQLEQKISSKLGSLKLMEQDTCNLEEEERKASTKIKEINVQKAKLVTELTNLIKICTSLHIQKVDLILQNTTVISEKNKLESDYMAASSQLRLTEQHFIELDENRQRLLQKCKELMKRARQVCNLGAEQTLPQEYQTQVPTIPNGHNSSLPMVFQDLPNTLDEIDALLTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS eid3_human EP300-interacting inhibitor of differentiation 3 EID-1-like inhibitor of differentiation 3 E1A-like inhibitor of differentiation 3 EID3 Non-structural maintenance of chromosomes element 4 homolog B protein protein human Homo sapiens Human MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEALEEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLSDCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDLSSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRLPILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY smc6-nsmce1 1268819 n/a 1268823 unspecified role unspecified role prey prey n/a 1268824 unspecified role unspecified role bait bait physical association physical association false false false 1C nsmce1-smc6 1268819 n/a 1268823 unspecified role unspecified role bait bait n/a 1268824 unspecified role unspecified role prey prey physical association physical association false false false 1C smc6-nsmce2 1268819 n/a 1268824 unspecified role unspecified role bait bait n/a 1268825 unspecified role unspecified role prey prey physical association physical association false false false 1E nsmce1-nsmce2 1268819 n/a 1268824 unspecified role unspecified role prey prey n/a 1268823 unspecified role unspecified role bait bait n/a 1268825 unspecified role unspecified role prey prey association association false false false 1E smc6-ndnl2-2 1268819 n/a 1268823 unspecified role unspecified role bait bait n/a 1268824 unspecified role unspecified role prey prey n/a 1268825 unspecified role unspecified role prey prey n/a 1268826 unspecified role unspecified role prey prey n/a 1268827 unspecified role unspecified role prey prey n/a 1268828 unspecified role unspecified role prey prey association association false false false 3A nsmce1-smc6-1 1268819 n/a 1268828 unspecified role unspecified role prey prey n/a 1268826 unspecified role unspecified role prey prey n/a 1268827 unspecified role unspecified role prey prey n/a 1268824 unspecified role unspecified role bait bait n/a 1268823 unspecified role unspecified role prey prey n/a 1268825 unspecified role unspecified role prey prey association association false false false 3A nsmce4a-nsmce1 1268820 n/a 1268823 unspecified role unspecified role prey prey n/a 1268824 unspecified role unspecified role prey prey n/a 1268826 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2B eid3-nsmce1 1268820 n/a 1268824 unspecified role unspecified role prey prey n/a 1268829 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1268823 unspecified role unspecified role prey prey association association false false false 2C ndnl2-nsmce1 1268820 n/a 1268823 unspecified role unspecified role prey prey n/a 1268824 unspecified role unspecified role prey prey n/a 1268827 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2D nse1-ndnl2 1268821 n/a 1268823 unspecified role unspecified role bait bait n/a 1268824 unspecified role unspecified role prey prey n/a 1268826 unspecified role unspecified role prey prey n/a 1268828 unspecified role unspecified role prey prey n/a 1268827 unspecified role unspecified role prey prey n/a 1268825 unspecified role unspecified role prey prey association association false false false Table I smc6-ndnl2-1 1268821 n/a 1268827 unspecified role unspecified role prey prey n/a 1268828 unspecified role unspecified role prey prey n/a 1268826 unspecified role unspecified role prey prey n/a 1268824 unspecified role unspecified role bait bait association association false false false Table I ndnl2-nsmce1-1 1268822 n/a 1268827 unspecified role unspecified role neutral component neutral component n/a 1268826 unspecified role unspecified role neutral component neutral component n/a 1268824 unspecified role unspecified role neutral component neutral component n/a 1268828 unspecified role unspecified role neutral component neutral component n/a 1268823 unspecified role unspecified role neutral component neutral component n/a 1268825 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3B