mpidb The Microbial Protein Interaction Database zheng-2007-2 Dissection of a type VI secretion system in Edwardsiella tarda. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zheng J., Leung K.Y. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation zheng-2007-1 Dissection of a type VI secretion system in Edwardsiella tarda. edwta Edwardsiella tarda anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zheng J., Leung K.Y. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation zheng-2007-3 Dissection of a type VI secretion system in Edwardsiella tarda. edwta Edwardsiella tarda pull down pull down western blot western blot Immuno blot Immuno blot Zheng J., Leung K.Y. Mol. Microbiol. (0950-382X) 2007 Only protein-protein interactions imex curation a8yqs1_edwta evpO protein protein edwta Edwardsiella tarda MAAMKRLFKWIAIVVLLSVLAMLCWLLTLWQRWPAAAAWVLFLAILAGALLTRYLWRKVRAWRTRSLEAEPVEKARAAERHPMTLQRQWKAATTLLKHSQLRRFRHPLQVLPWFMLLGRPGSGKSALLAQSGLAAPLDLPHELNDGKALVDWWYFDKAVILDISGQFVQPEASQETRSTWNRMLDLLGRDRARAGLNGLVLTVSAQTLLSADGGGVVDEGRLIRTRIEQLIRLFDRRFPVYVLVTQIDSLYGMEAWSHLLTPEALDQAMGHVVDEPENGRDVRDVIDIALGRVNSRLKTMQLTMLLQHPDADPALLMFPTELARLAEPLKRYVYHALGDSPYLESPILRGVFFASAVQKGNTRSGPEDQQEIALQEAAHDTVKRGVFVRDLFNRVLPADRHLNRPVAVTSPWRRLNRHLGLFAWWLCVAAALGYLGVSVVYWQHTLDDLRQRYPATMAMNRALPHDMQMMIAQRDAIYWLENRNAQWASRVLAFQHQLLSVEADAKSQYVNDFSQYILPSLDKAIQQSTTADSIASHPDGLVNAIQVEVRRANLLRARLRGSSYGALMAMPPIPTPPLRLLYPALDSDTVSRFNDLFLAQLAWSTDIQQQNARLLHLQARLRQLAAQSATLDWIIPWANAQPDLTNVTLDDFWQGSLRPDKLPEVDAAYTLEGQQRIRAFLVEMRSSMPNDIAFDKKRAAFWHEYAQKRLQAWQDFAGQFSQGERILAGQGEWQSTIDRLPHDDNPYLRLARRLLTEFAEMPDDLRPGWLRVLPQWLEIRQRAHQQALVRVPGLVSDVGGMILRGTAQKGALDQTRSQLVGRLDAIRLYQEYQAAMTQAMNSVVISQAQATKVAADFHGFDSQQSGQSSLQTAYARLMSLRSLLGYNRPDEQLTWGLMAGPLQLVIRYADQQASCFLQSAWQDRVIAPSRWTLNQAERADKLYGDNGLLWTFLSGPIKPFIQRNDSGWQEVDTLGQSVPFTADFLPFINRALIRQVDLKAGKIALHQKQQQATLAQQQQKQRWQTVAAESGEKLAELNTAAAALKAKSYPVALTGLPTDVNSNAKAKVVGTLLTMQCTNATLRLNNLNFAVMQSVLWSQQQCGQVTLQIRLPTFTLTRSYPGPQGFVHFLRDFPGGARRFTPADFPVDKSRMAAVGLTQITVRYRIAGQETLLQDAAQLAQAERDIQSAQTRQQQAQQALLTLNRPSASESLPPLDTLPGLTMMSVPERIGQCWRGDIRLARENDGIGRLMSELVAKQLSDKP a8yqs0_edwta evpN protein protein edwta Edwardsiella tarda MSLSEAFIQPMLYVRQYLQAPEGEPSLFRERLQSYLHHSQLRAQEAGESPAAIDSALYAVVAWMDETIMCSTWDGVATWRRDPLQASYFNTVCAGVDFFDKLTALSPEADDVREVYLLCLALGFEGRYAGPTGQTALTQIRVHQLRDLHGEVWEASRRLFPEAYPRSSVTPTQQTRWLTQSRFTTVVVPLVILVALYGILNWVLYEYADLALEWLP a8yqr8_edwta evpL protein protein edwta Edwardsiella tarda MSGSERWWKMTAPGGCSKVYWAMMKNKAIWSAIGCLLLSLSGCTSDAAKQQARSSVSWRYGSQAILVNAQTAPDLNSYDGESHSLVLAVVQTRSPDAFYQLIQTPELAGLVLQGGRPPDGILQVSRYALEPGHAFRLVLDRLQEARQVGFIAAFYGLPLTQTSRLFDIPVSVTRSGILFPDWQAAPSPLALDLKLGNSGIGEAESHPLERIPAAPDPASAPTAATPIRLSNSGEP q6ee21_edwta evpA protein protein edwta Edwardsiella tarda MASESKQHTLDRVRSPRVQITYDVEIGDAQEMKELPFVMGVLGDYSGQPATPLPKLKERKFVSIDRDNFNDVIKGVHPHLSFRTENTLSGDDSQLSVDLHFQSMADFTPERVAAQVEPLRKLMDIRSRLSDLKNKMYSNERLGEVLQGIIEDTEKLQSLGKETGFGEEKQP a8yqr4_edwta evpP protein protein edwta Edwardsiella tarda MSILNSELDWSHVGSISTGPGTVVSDAFNISYGLPTKELLPAGTALYKFNGFSSLARPPITDDTPLSPWWSPVQPFRHDGGLQQRMLVAKLNGVSMREWGRLTSVIKENWSSLDHLLEIVLKVPVYAWFGGFKGMSRIDNGMPSKRNITLEQKGRGSNLPGGATQFYIPNLTVGHISSHNFSALK q6ee19_edwta evpC protein protein edwta Edwardsiella tarda MAFDTYIKLDKVDGESTDDKHKKWIEVLGFAWGAGNECTMESGTQGLNTGKAMMSVLRVTKWMDCASVKLASAAVQGQNFPTLELEICTQAGDKFAFCIYKFTHVAVSSYQCSGATGGSDRPQETIDFAYKEVTWEYVPQDQNGKAGGKIGPEGWSLITNKKK evpo-evpn 1332303 not-defined-558245991 1332306 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-615872721 1332307 unspecified role unspecified role prey prey region A8YQS0-1-182 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1332316 1332314 physical association physical association false false false Fig. 5C evpo-evpl 1332303 not-defined-334684754 1332306 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-366445402 1332308 unspecified role unspecified role prey prey physical association physical association false false false evpo-evpa 1332303 not-defined-104357597 1332309 unspecified role unspecified role prey prey not-defined-1198576891 1332306 unspecified role unspecified role bait bait region A8YQS1-444-1263 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5 evpp-evpc 1332304 not-defined-752614509 1332310 unspecified role unspecified role bait bait not-defined-1942715921 1332311 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A evpp-evpc-1 1332305 not-defined-752614509 1332310 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1942715921 1332311 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A