UniProt UniProt http://www.uniprot.org wu-2007d-3 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation wu-2007d-1 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation wu-2007d-2 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation wu-2007d-4 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation wu-2007d-5 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation wu-2007d-6 Membrane glycoprotein M6a interacts with the micro-opioid receptor and facilitates receptor endocytosis and recycling. rat-brain Rattus norvegicus brain brain Brain [cerebrum] confocal microscopy confocal microscopy immunostaining immunostaining Wu D.F., Koch T., Liang Y.J., Stumm R., Schulz S., Schroder H., Hollt V. thomas.koch@Medizin.Uni-Magdeburg.de J. Biol. Chem. (0021-9258) 2007 mimix curation oprm_rat Mu-type opioid receptor Oprm1 Ror-b Opioid receptor B protein protein rat Rattus norvegicus (Rat) Rat MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLP gpm6a_rat Neuronal membrane glycoprotein M6-a Gpm6a m6a protein protein rat Rattus norvegicus (Rat) Rat MEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARTAGDTLDVFTMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLPVYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTVSENFLRMCESTELNMTFHLFIVALAGAGAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT oprm1-gpm6a-2 1288566 n/a 1288572 unspecified role unspecified role bait bait n/a 1288573 unspecified role unspecified role prey prey physical association physical association false false false 1B gpm6a-oprm1 1288566 n/a 1288573 unspecified role unspecified role bait bait n/a 1288572 unspecified role unspecified role prey prey physical association physical association false false false 1B oprm1-gpm6a 1288567 n/a 1288572 unspecified role unspecified role bait bait n/a 1288573 unspecified role unspecified role prey prey physical association physical association false false false no figure oprm1-gpm6a-1 1288568 n/a 1288572 unspecified role unspecified role bait bait n/a 1288573 unspecified role unspecified role prey prey physical association physical association false false false 1A gpm6a-oprm1-1 1288569 n/a 1288572 unspecified role unspecified role fluorescence donor fluorescence donor n/a 1288573 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept proximity proximity close-contact colocalization neighbourhood interaction false false false 1C, 2B and C oprm1-gpm6a-3 1288570 n/a 1288572 unspecified role unspecified role bait bait n/a 1288573 unspecified role unspecified role prey prey direct interaction direct interaction false false false 3 gpm6a-oprm1-2 1288571 n/a 1288573 unspecified role unspecified role neutral component neutral component n/a 1288572 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6D and 7 Monensin and DAMGO treatment enhance colocalization in cytoplasmuc vesicles.