mpidb The Microbial Protein Interaction Database balasingham-2007-1 Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis. ecolx Escherichia coli far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Balasingham SV, Collins RF, Assalkhou R, Homberset H, Frye SA, Derrick JP, Tønjum T J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation balasingham-2007-2 Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy tag visualisation tag visualisation Balasingham SV, Collins RF, Assalkhou R, Homberset H, Frye SA, Derrick JP, Tønjum T J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation pilq_neimb Type IV pilus biogenesis and competence protein PilQ pilQ NMB1812 protein protein strain mc58 Neisseria meningitidis serogroup B MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAVSVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY q7dd77_neimb pilP NMB1811 protein protein strain mc58 Neisseria meningitidis serogroup B MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAPTLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILKSGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAELLLNSSDKNTEQAAAPAAEQN pilp-pilq-1 1331871 not-defined-2146113209 1331873 unspecified role unspecified role prey prey region Q70M91-217-478 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-39689536 1331874 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q7DD77-81-102 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false cys-16 Q7DD77-1-16 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 7 and table 3 pilp-pilq-3 1331872 not-defined-774950169 1331873 unspecified role unspecified role neutral component neutral component not-defined-13684385 1331874 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 8